Values CatLog read from this paper
Identification of the catalytic residues in family 52 glycoside hydrolase, a beta-xylosidase from Geobacillus stearothermophilus T-6
PMID 12738774doi:10.1074/jbc.m304144200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 59 records (73 values) from this paper: β-xylosidase XynB2 from Geobacillus stearothermophilus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| β-xylosidase XynB2 | Geobacillus stearothermophilus | 2,4,6-trichlorophenyl beta-D-xylopyranoside | 61 | 0.81 | 75 | pH 7, 40 °C | Open record: β-xylosidase XynB2, 2,4,6-trichlorophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2 | Geobacillus stearothermophilus | 2,5-dinitrophenyl beta-D-xylopyranoside | 64 | 0.67 | 95 | pH 7, 40 °C | Open record: β-xylosidase XynB2, 2,5-dinitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2 | Geobacillus stearothermophilus | 2-nitrophenyl beta-D-xylopyranoside | 73 | 0.46 | 60 | pH 7, 40 °C | Open record: β-xylosidase XynB2, 2-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2 | Geobacillus stearothermophilus | 3,4-dinitrophenyl beta-D-xylopyranoside | 50 | 0.15 | 330 | pH 7, 40 °C | Open record: β-xylosidase XynB2, 3,4-dinitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2 | Geobacillus stearothermophilus | 3-nitrophenyl beta-D-xylopyranoside | 41 | 0.87 | 47 | pH 7, 40 °C | Open record: β-xylosidase XynB2, 3-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2 | Geobacillus stearothermophilus | 4-methylumbelliferyl beta-D-xylopyranoside | 80 | 0.35 | 230 | pH 7, 40 °C | Open record: β-xylosidase XynB2, 4-methylumbelliferyl beta-D-xylopyranoside |
| β-xylosidase XynB2 | Geobacillus stearothermophilus | 4-nitrophenyl beta-D-xylopyranoside | 18 | 0.13 | 140 | pH 7, 40 °C | Open record: β-xylosidase XynB2, 4-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 2,4,6-trichlorophenyl beta-D-xylopyranoside | 0.063 | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 2,4,6-trichlorophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 2,4,6-trichlorophenyl beta-D-xylopyranoside | — | 0.001 | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 2,4,6-trichlorophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 2,4,6-trichlorophenyl beta-D-xylopyranoside | — | — | 63 | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 2,4,6-trichlorophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 2,4,6-trichlorophenyl beta-D-xylopyranosidewith 0.63 M sodium azide (chemical rescue) | 21 | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 2,4,6-trichlorophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 2,4,6-trichlorophenyl beta-D-xylopyranosidewith 0.63 M sodium azide (chemical rescue) | — | 0.18 | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 2,4,6-trichlorophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 2,4,6-trichlorophenyl beta-D-xylopyranosidewith 0.63 M sodium azide (chemical rescue) | — | — | 117 | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 2,4,6-trichlorophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 2,5-dinitrophenyl beta-D-xylopyranoside | 0.057 | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 2,5-dinitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 2,5-dinitrophenyl beta-D-xylopyranoside | — | 0.0017 | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 2,5-dinitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 2,5-dinitrophenyl beta-D-xylopyranoside | — | — | 34 | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 2,5-dinitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 2,5-dinitrophenyl beta-D-xylopyranosidewith 0.63 M sodium azide (chemical rescue) | 52 | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 2,5-dinitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 2,5-dinitrophenyl beta-D-xylopyranosidewith 0.63 M sodium azide (chemical rescue) | — | 0.053 | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 2,5-dinitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 2,5-dinitrophenyl beta-D-xylopyranosidewith 0.63 M sodium azide (chemical rescue) | — | — | 974 | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 2,5-dinitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 2-nitrophenyl beta-D-xylopyranoside | 0.067 | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 2-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 2-nitrophenyl beta-D-xylopyranoside | — | 0.0034 | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 2-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 2-nitrophenyl beta-D-xylopyranoside | — | — | 20 | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 2-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 2-nitrophenyl beta-D-xylopyranosidewith 0.63 M sodium azide (chemical rescue) | 2.2 | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 2-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 2-nitrophenyl beta-D-xylopyranosidewith 0.63 M sodium azide (chemical rescue) | — | 0.035 | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 2-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 2-nitrophenyl beta-D-xylopyranosidewith 0.63 M sodium azide (chemical rescue) | — | — | 6 | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 2-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 3,4-dinitrophenyl beta-D-xylopyranoside | 0.088 | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 3,4-dinitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 3,4-dinitrophenyl beta-D-xylopyranoside | — | 3 × 10⁻⁴ | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 3,4-dinitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 3,4-dinitrophenyl beta-D-xylopyranoside | — | — | 295 | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 3,4-dinitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 3,4-dinitrophenyl beta-D-xylopyranosidewith 0.63 M sodium azide (chemical rescue) | 27 | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 3,4-dinitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 3,4-dinitrophenyl beta-D-xylopyranosidewith 0.63 M sodium azide (chemical rescue) | — | 0.045 | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 3,4-dinitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 3,4-dinitrophenyl beta-D-xylopyranosidewith 0.63 M sodium azide (chemical rescue) | — | — | 594 | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 3,4-dinitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 3-nitrophenyl beta-D-xylopyranoside | 0.021 | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 3-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 3-nitrophenyl beta-D-xylopyranoside | — | 0.061 | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 3-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 3-nitrophenyl beta-D-xylopyranoside | — | — | 0.34 | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 3-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 3-nitrophenyl beta-D-xylopyranosidewith 0.63 M sodium azide (chemical rescue) | 0.026 | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 3-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 3-nitrophenyl beta-D-xylopyranosidewith 0.63 M sodium azide (chemical rescue) | — | 0.099 | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 3-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 3-nitrophenyl beta-D-xylopyranosidewith 0.63 M sodium azide (chemical rescue) | — | — | 0.27 | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 3-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 4-methylumbelliferyl beta-D-xylopyranoside | 0.055 | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 4-methylumbelliferyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 4-methylumbelliferyl beta-D-xylopyranoside | — | 0.032 | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 4-methylumbelliferyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 4-methylumbelliferyl beta-D-xylopyranoside | — | — | 1.7 | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 4-methylumbelliferyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 4-methylumbelliferyl beta-D-xylopyranosidewith 0.63 M sodium azide (chemical rescue) | 0.2 | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 4-methylumbelliferyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 4-methylumbelliferyl beta-D-xylopyranosidewith 0.63 M sodium azide (chemical rescue) | — | 0.12 | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 4-methylumbelliferyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 4-methylumbelliferyl beta-D-xylopyranosidewith 0.63 M sodium azide (chemical rescue) | — | — | 1.6 | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 4-methylumbelliferyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 4-nitrophenyl beta-D-xylopyranoside | 0.039 | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 4-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 4-nitrophenyl beta-D-xylopyranoside | — | 0.0042 | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 4-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 4-nitrophenyl beta-D-xylopyranoside | — | — | 9.2 | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 4-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 4-nitrophenyl beta-D-xylopyranosidewith 0.63 M sodium azide (chemical rescue) | 0.42 | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 4-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 4-nitrophenyl beta-D-xylopyranosidewith 0.63 M sodium azide (chemical rescue) | — | 0.019 | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 4-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant D495G | Geobacillus stearothermophilus | 4-nitrophenyl beta-D-xylopyranosidewith 0.63 M sodium azide (chemical rescue) | — | — | 22 | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant D495G, 4-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant E335A | Geobacillus stearothermophilus | 2,5-dinitrophenyl beta-D-xylopyranoside | 1.7 × 10⁻⁴ | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant E335A, 2,5-dinitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant E335A | Geobacillus stearothermophilus | 2,5-dinitrophenyl beta-D-xylopyranosidewith 1.4 M sodium azide (chemical rescue) | 0.0056 | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant E335A, 2,5-dinitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant E335A | Geobacillus stearothermophilus | 4-nitrophenyl beta-D-xylopyranoside | 3.4 × 10⁻⁶ | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant E335A, 4-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant E335G | Geobacillus stearothermophilus | 2,5-dinitrophenyl beta-D-xylopyranoside | 7.5 × 10⁻⁴ | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant E335G, 2,5-dinitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant E335G | Geobacillus stearothermophilus | 2,5-dinitrophenyl beta-D-xylopyranoside1.4 M sodium azide | 0.33 | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant E335G, 2,5-dinitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant E335G | Geobacillus stearothermophilus | 2,5-dinitrophenyl beta-D-xylopyranoside2.3 M formate | 0.67 | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant E335G, 2,5-dinitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant E335G | Geobacillus stearothermophilus | 4-nitrophenyl beta-D-xylopyranoside | 2 × 10⁻⁵ | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant E335G, 4-nitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant E335Q | Geobacillus stearothermophilus | 2,5-dinitrophenyl beta-D-xylopyranoside | 4.2 × 10⁻⁴ | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant E335Q, 2,5-dinitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant E335Q | Geobacillus stearothermophilus | 2,5-dinitrophenyl beta-D-xylopyranosidewith 1.4 M sodium azide (chemical rescue) | 0.0019 | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant E335Q, 2,5-dinitrophenyl beta-D-xylopyranoside |
| β-xylosidase XynB2variant E335Q | Geobacillus stearothermophilus | 4-nitrophenyl beta-D-xylopyranoside | 8.1 × 10⁻⁵ | — | — | pH 7, 40 °C | Open record: β-xylosidase XynB2 variant E335Q, 4-nitrophenyl beta-D-xylopyranoside |