Values CatLog read from this paper
Key amino acid residues in the regulation of soluble methane monooxygenase catalysis by component B
PMID 12741818doi:10.1021/bi027429iOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 19 records (19 values) from this paper: soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR) from Methylosinus trichosporium.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR) | Methylosinus trichosporium | furan | 1.13 | — | — | pH 7.6, 25 °C | Open record: soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR), furan |
| soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR) | Methylosinus trichosporium | methane | 1.05 | — | — | pH 7.6, 25 °C | Open record: soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR), methane |
| soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR) | Methylosinus trichosporium | nitrobenzene | 0.3 | — | — | pH 7.6, 25 °C | Open record: soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR), nitrobenzene |
| soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR)variant MMOB N107A/S110A (DBL1) | Methylosinus trichosporium | furan | 0.56 | — | — | pH 7.6, 25 °C | Open record: soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR) variant MMOB N107A/S110A (DBL1), furan |
| soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR)variant MMOB N107A/S110A (DBL1) | Methylosinus trichosporium | methane | 0.52 | — | — | pH 7.6, 25 °C | Open record: soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR) variant MMOB N107A/S110A (DBL1), methane |
| soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR)variant MMOB N107A/S110A (DBL1) | Methylosinus trichosporium | nitrobenzene | 0.1 | — | — | pH 7.6, 25 °C | Open record: soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR) variant MMOB N107A/S110A (DBL1), nitrobenzene |
| soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR)variant MMOB N107G/S109A/S110A/T111A (Quad) | Methylosinus trichosporium | nitrobenzene | 0.5 | — | — | pH 7.6, 25 °C | Open record: soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR) variant MMOB N107G/S109A/S110A/T111A (Quad), nitrobenzene |
| soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR)variant MMOB S109A | Methylosinus trichosporium | furan | 1.11 | — | — | pH 7.6, 25 °C | Open record: soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR) variant MMOB S109A, furan |
| soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR)variant MMOB S109A | Methylosinus trichosporium | methane | 0.89 | — | — | pH 7.6, 25 °C | Open record: soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR) variant MMOB S109A, methane |
| soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR)variant MMOB S109A | Methylosinus trichosporium | nitrobenzene | 0.35 | — | — | pH 7.6, 25 °C | Open record: soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR) variant MMOB S109A, nitrobenzene |
| soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR)variant MMOB S109A/T111A (DBL2) | Methylosinus trichosporium | furan | 1.4 | — | — | pH 7.6, 25 °C | Open record: soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR) variant MMOB S109A/T111A (DBL2), furan |
| soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR)variant MMOB S109A/T111A (DBL2) | Methylosinus trichosporium | methane | 1.11 | — | — | pH 7.6, 25 °C | Open record: soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR) variant MMOB S109A/T111A (DBL2), methane |
| soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR)variant MMOB S109A/T111A (DBL2) | Methylosinus trichosporium | nitrobenzene | 0.5 | — | — | pH 7.6, 25 °C | Open record: soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR) variant MMOB S109A/T111A (DBL2), nitrobenzene |
| soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR)variant MMOB T111A | Methylosinus trichosporium | furan | 0.79 | — | — | pH 7.6, 25 °C | Open record: soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR) variant MMOB T111A, furan |
| soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR)variant MMOB T111A | Methylosinus trichosporium | methane | 0.79 | — | — | pH 7.6, 25 °C | Open record: soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR) variant MMOB T111A, methane |
| soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR)variant MMOB T111A | Methylosinus trichosporium | nitrobenzene | 0.38 | — | — | pH 7.6, 25 °C | Open record: soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR) variant MMOB T111A, nitrobenzene |
| soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR)variant MMOB T111Y | Methylosinus trichosporium | furan | 0.15 | — | — | pH 7.6, 25 °C | Open record: soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR) variant MMOB T111Y, furan |
| soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR)variant MMOB T111Y | Methylosinus trichosporium | methane | 0.12 | — | — | pH 7.6, 25 °C | Open record: soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR) variant MMOB T111Y, methane |
| soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR)variant MMOB T111Y | Methylosinus trichosporium | nitrobenzene | 0.04 | — | — | pH 7.6, 25 °C | Open record: soluble methane monooxygenase (sMMO; reconstituted hydroxylase MMOH with component B MMOB and reductase MMOR) variant MMOB T111Y, nitrobenzene |