CatLogenzyme kinetics catalog

Values CatLog read from this paper

Structural determinants in human DNA polymerase gamma account for mitochondrial toxicity from nucleoside analogs

PMID 12742017doi:10.1016/s0022-2836(03)00405-4Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 48 records (102 values) from this paper: DNA polymerase γ (pol γ), catalytic subunit from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 42 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.7.7.7

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
DNA polymerase γ (pol γ), catalytic subunitHomo sapiens(−)-2'-deoxy-3'-thiacytidine 5'-triphosphate0.00170.0150.11 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitHomo sapiens2',3'-didehydro-2',3'-dideoxythymidine 5'-triphosphate0.0175.5 × 10⁻⁵300 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitHomo sapiens2',3'-dideoxycytidine 5'-triphosphate0.0351.3 × 10⁻⁴270 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitHomo sapiens2'-deoxycytidine 5'-triphosphate0.0482.5 × 10⁻⁵1.9 × 10³ calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitHomo sapiens2'-deoxyguanosine 5'-triphosphate0.0476 × 10⁻⁶7.8 × 10³ calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitHomo sapiens3'-azido-3'-deoxythymidine 5'-triphosphate0.00830.0930.090 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitHomo sapienscarbovir 5'-triphosphate0.0040.00430.93 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitHomo sapiensthymidine 5'-triphosphate3.70.00261.4 × 10³37 °COpen
DNA polymerase γ (pol γ), catalytic subunitHomo sapiensthymidine 5'-triphosphate0.043.9 × 10⁻⁵1.0 × 10³ calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant E895AHomo sapiens2',3'-didehydro-2',3'-dideoxythymidine 5'-triphosphate8.3 × 10⁻⁵0.0640.0013 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant E895AHomo sapiens2',3'-dideoxycytidine 5'-triphosphate2.3 × 10⁻⁴0.230.0010 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant E895AHomo sapiens2'-deoxycytidine 5'-triphosphate1.5 × 10⁻⁴0.00120.12 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant E895AHomo sapiens2'-deoxyguanosine 5'-triphosphate3 × 10⁻⁴2.2 × 10⁻⁴1.4 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant E895AHomo sapiensthymidine 5'-triphosphate0.490.14337 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant E895AHomo sapiensthymidine 5'-triphosphate6.7 × 10⁻⁴3.4 × 10⁻⁴2.0 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y951AHomo sapiens2',3'-didehydro-2',3'-dideoxythymidine 5'-triphosphate8 × 10⁻⁴0.0160.050 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y951AHomo sapiens2',3'-dideoxycytidine 5'-triphosphate0.00180.320.0057 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y951AHomo sapiens2'-deoxycytidine 5'-triphosphate0.00470.00770.61 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y951AHomo sapiens2'-deoxyguanosine 5'-triphosphate0.00954.8 × 10⁻⁴20 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y951AHomo sapiens3'-azido-3'-deoxythymidine 5'-triphosphate6.7 × 10⁻⁵0.050.0013 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y951AHomo sapienscarbovir 5'-triphosphate5 × 10⁻⁵0.212.4 × 10⁻⁴ calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y951AHomo sapiensthymidine 5'-triphosphate0.10.2137 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y951AHomo sapiensthymidine 5'-triphosphate0.001420.00180.79 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y951FHomo sapiens2',3'-didehydro-2',3'-dideoxythymidine 5'-triphosphate0.001120.00930.12 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y951FHomo sapiens2',3'-dideoxycytidine 5'-triphosphate4.3 × 10⁻⁴0.0110.039 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y951FHomo sapiens2'-deoxycytidine 5'-triphosphate0.0771.1 × 10⁻⁴700 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y951FHomo sapiens2'-deoxyguanosine 5'-triphosphate0.186.4 × 10⁻⁵2.9 × 10³ calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y951FHomo sapiens3'-azido-3'-deoxythymidine 5'-triphosphate7.2 × 10⁻⁴0.0260.028 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y951FHomo sapiensthymidine 5'-triphosphate1.80.00290037 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y951FHomo sapiensthymidine 5'-triphosphate0.0141.8 × 10⁻⁵780 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y955AHomo sapiens(−)-2'-deoxy-3'-thiacytidine 5'-triphosphate2.5 × 10⁻⁴0.030.0083 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y955AHomo sapiens2',3'-didehydro-2',3'-dideoxythymidine 5'-triphosphate0.00220.0420.052 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y955AHomo sapiens2',3'-dideoxycytidine 5'-triphosphate0.01483.7 × 10⁻⁴40 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y955AHomo sapiens2'-deoxycytidine 5'-triphosphate0.0235 × 10⁻⁵470 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y955AHomo sapiens2'-deoxyguanosine 5'-triphosphate0.0132 × 10⁻⁵650 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y955AHomo sapiens3'-azido-3'-deoxythymidine 5'-triphosphate5 × 10⁻⁴0.0670.0075 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y955AHomo sapienscarbovir 5'-triphosphate8.3 × 10⁻⁵0.0450.0019 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y955AHomo sapiensthymidine 5'-triphosphate5.80.173037 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y955AHomo sapiensthymidine 5'-triphosphate0.0181.4 × 10⁻⁴130 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y955FHomo sapiens(−)-2'-deoxy-3'-thiacytidine 5'-triphosphate0.001020.010.10 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y955FHomo sapiens2',3'-didehydro-2',3'-dideoxythymidine 5'-triphosphate0.0110.00176.5 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y955FHomo sapiens2',3'-dideoxycytidine 5'-triphosphate0.026.4 × 10⁻⁵310 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y955FHomo sapiens2'-deoxycytidine 5'-triphosphate0.033 × 10⁻⁵1.0 × 10³ calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y955FHomo sapiens2'-deoxyguanosine 5'-triphosphate0.0431.5 × 10⁻⁵2.9 × 10³ calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y955FHomo sapiens3'-azido-3'-deoxythymidine 5'-triphosphate0.00370.0550.067 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y955FHomo sapienscarbovir 5'-triphosphate0.0030.00380.79 calc.pH 7.5, 37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y955FHomo sapiensthymidine 5'-triphosphate3.20.00271.2 × 10³37 °COpen
DNA polymerase γ (pol γ), catalytic subunitvariant Y955FHomo sapiensthymidine 5'-triphosphate0.01421.8 × 10⁻⁵790 calc.pH 7.5, 37 °COpen