Values CatLog read from this paper

Human DNA polymerase lambda diverged in evolution from DNA polymerase beta toward specific Mn(++) dependence: a kinetic and thermodynamic study

PMID 12809503doi:10.1021/bi034198mOpen in Browse

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CatLog read 8 records (24 values) from this paper: DNA polymerase λ from Homo sapiens.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.7.7.7

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
DNA polymerase λ	Homo sapiens	2′-deoxythymidine triphosphate1 mM MnCl2 (Mn2+ activator)	0.016	0.0032	5	pH 7.6, 37 °C	Open record: DNA polymerase λ, 2′-deoxythymidine triphosphate
DNA polymerase λ	Homo sapiens	2′-deoxythymidine triphosphate1 mM MgCl2 (Mg2+ activator)	0.006	0.0047	1.2	pH 7.6, 37 °C	Open record: DNA polymerase λ, 2′-deoxythymidine triphosphate
DNA polymerase λ	Homo sapiens	DNA primer 3′-OH ends (primer/template DNA substrate)1 mM MnCl2 (Mn2+ activator)	0.02	7.5 × 10⁻⁵	280	pH 7.6, 37 °C	Open record: DNA polymerase λ, DNA primer 3′-OH ends (primer/template DNA substrate)
DNA polymerase λ	Homo sapiens	DNA primer 3′-OH ends (primer/template DNA substrate)1 mM MgCl2 (Mg2+ activator)	0.004	4.3 × 10⁻⁵	90	pH 7.6, 37 °C	Open record: DNA polymerase λ, DNA primer 3′-OH ends (primer/template DNA substrate)
DNA polymerase λvariant ΔN (DNA pol λΔN, aa 244-575)	Homo sapiens	2′-deoxythymidine triphosphate1 mM MnCl2 (Mn2+ activator)	0.016	0.0069	2.2	pH 7.6, 37 °C	Open record: DNA polymerase λ variant ΔN (DNA pol λΔN, aa 244-575), 2′-deoxythymidine triphosphate
DNA polymerase λvariant ΔN (DNA pol λΔN, aa 244-575)	Homo sapiens	2′-deoxythymidine triphosphate1 mM MgCl2 (Mg2+ activator)	0.005	0.011	0.4	pH 7.6, 37 °C	Open record: DNA polymerase λ variant ΔN (DNA pol λΔN, aa 244-575), 2′-deoxythymidine triphosphate
DNA polymerase λvariant ΔN (DNA pol λΔN, aa 244-575)	Homo sapiens	DNA primer 3′-OH ends (primer/template DNA substrate)1 mM MnCl2 (Mn2+ activator)	0.018	1.5 × 10⁻⁵	1.2 × 10³	pH 7.6, 37 °C	Open record: DNA polymerase λ variant ΔN (DNA pol λΔN, aa 244-575), DNA primer 3′-OH ends (primer/template DNA substrate)
DNA polymerase λvariant ΔN (DNA pol λΔN, aa 244-575)	Homo sapiens	DNA primer 3′-OH ends (primer/template DNA substrate)1 mM MgCl2 (Mg2+ activator)	0.007	4.2 × 10⁻⁵	200	pH 7.6, 37 °C	Open record: DNA polymerase λ variant ΔN (DNA pol λΔN, aa 244-575), DNA primer 3′-OH ends (primer/template DNA substrate)