Values CatLog read from this paper
Probing the roles of key residues in the unique regulatory NADH binding site of type II citrate synthase of Escherichia coli
PMID 12824188doi:10.1074/jbc.m302786200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 18 records (27 values) from this paper: citrate synthase (Type II) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 9 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| citrate synthase (Type II)variant H110A | Escherichia coli | acetyl coenzyme A | 67 | 0.049 | 1.4 × 10³ calc. | Open record: citrate synthase (Type II) variant H110A, acetyl coenzyme A | |
| citrate synthase (Type II)variant H110A | Escherichia coli | oxaloacetate | — | 0.003 | — | Open record: citrate synthase (Type II) variant H110A, oxaloacetate | |
| citrate synthase (Type II)variant K167A | Escherichia coli | acetyl coenzyme A | 84 | 0.22 | 380 calc. | Open record: citrate synthase (Type II) variant K167A, acetyl coenzyme A | |
| citrate synthase (Type II)variant K167A | Escherichia coli | oxaloacetate | — | 0.037 | — | Open record: citrate synthase (Type II) variant K167A, oxaloacetate | |
| citrate synthase (Type II)variant N189A | Escherichia coli | acetyl coenzyme A | 124 | 0.069 | 1.8 × 10³ calc. | Open record: citrate synthase (Type II) variant N189A, acetyl coenzyme A | |
| citrate synthase (Type II)variant N189A | Escherichia coli | oxaloacetate | — | 0.017 | — | Open record: citrate synthase (Type II) variant N189A, oxaloacetate | |
| citrate synthase (Type II)variant Q182A | Escherichia coli | acetyl coenzyme A | 95 | 0.056 | 1.7 × 10³ calc. | Open record: citrate synthase (Type II) variant Q182A, acetyl coenzyme A | |
| citrate synthase (Type II)variant Q182A | Escherichia coli | oxaloacetate | — | 0.01 | — | Open record: citrate synthase (Type II) variant Q182A, oxaloacetate | |
| citrate synthase (Type II)variant R109L | Escherichia coli | acetyl coenzyme A | 121 | 0.069 | 1.8 × 10³ calc. | Open record: citrate synthase (Type II) variant R109L, acetyl coenzyme A | |
| citrate synthase (Type II)variant R109L | Escherichia coli | oxaloacetate | — | 0.007 | — | Open record: citrate synthase (Type II) variant R109L, oxaloacetate | |
| citrate synthase (Type II)variant R163L | Escherichia coli | acetyl coenzyme A | 108 | 0.08 | 1.4 × 10³ calc. | Open record: citrate synthase (Type II) variant R163L, acetyl coenzyme A | |
| citrate synthase (Type II)variant R163L | Escherichia coli | oxaloacetate | — | 0.005 | — | Open record: citrate synthase (Type II) variant R163L, oxaloacetate | |
| citrate synthase (Type II)variant T111A | Escherichia coli | acetyl coenzyme A | 54 | 0.079 | 680 calc. | Open record: citrate synthase (Type II) variant T111A, acetyl coenzyme A | |
| citrate synthase (Type II)variant T111A | Escherichia coli | oxaloacetate | — | 0.018 | — | Open record: citrate synthase (Type II) variant T111A, oxaloacetate | |
| citrate synthase (Type II)variant T204A | Escherichia coli | acetyl coenzyme A | 118 | 0.21 | 560 calc. | Open record: citrate synthase (Type II) variant T204A, acetyl coenzyme A | |
| citrate synthase (Type II)variant T204A | Escherichia coli | oxaloacetate | — | 0.004 | — | Open record: citrate synthase (Type II) variant T204A, oxaloacetate | |
| citrate synthase (Type II)variant Y145A | Escherichia coli | acetyl coenzyme A | 36 | 0.23 | 160 calc. | Open record: citrate synthase (Type II) variant Y145A, acetyl coenzyme A | |
| citrate synthase (Type II)variant Y145A | Escherichia coli | oxaloacetate | — | 0.051 | — | Open record: citrate synthase (Type II) variant Y145A, oxaloacetate |