Values CatLog read from this paper

Activity of hyperthermophilic glycosynthases is significantly enhanced at acidic pH

PMID 12859194doi:10.1021/bi0345384Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 19 records (57 values) from this paper: β-glycosidase (Taβ-gly), β-glycosidase (Ssβ-gly), β-glycosidase CelB from Thermosphaera aggregans, Sulfolobus solfataricus, Pyrococcus furiosus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.2.1.21

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
β-glycosidase (Ssβ-gly)	Sulfolobus solfataricus	2-nitrophenyl-β-D-glucopyranoside50 mM sodium phosphate buffer	538	1.01	533	pH 6.5, 65 °C	Open record: β-glycosidase (Ssβ-gly), 2-nitrophenyl-β-D-glucopyranoside
β-glycosidase (Ssβ-gly)	Sulfolobus solfataricus	2-nitrophenyl-β-D-glucopyranoside	425	0.5	850	pH 6.5, 65 °C	Open record: β-glycosidase (Ssβ-gly), 2-nitrophenyl-β-D-glucopyranoside
β-glycosidase (Ssβ-gly)variant E387G	Sulfolobus solfataricus	2-nitrophenyl-β-D-glucopyranoside	901.4	16.4	55	pH 3, 65 °C	Open record: β-glycosidase (Ssβ-gly) variant E387G, 2-nitrophenyl-β-D-glucopyranoside
β-glycosidase (Ssβ-gly)variant E387G	Sulfolobus solfataricus	2-nitrophenyl-β-D-glucopyranoside	321.9	4.9	65.9	pH 4, 65 °C	Open record: β-glycosidase (Ssβ-gly) variant E387G, 2-nitrophenyl-β-D-glucopyranoside
β-glycosidase (Ssβ-gly)variant E387G	Sulfolobus solfataricus	2-nitrophenyl-β-D-glucopyranoside	52.9	2.2	23.8	pH 5, 65 °C	Open record: β-glycosidase (Ssβ-gly) variant E387G, 2-nitrophenyl-β-D-glucopyranoside
β-glycosidase (Ssβ-gly)variant E387G	Sulfolobus solfataricus	2-nitrophenyl-β-D-glucopyranoside	20.7	1.1	18.5	pH 6, 65 °C	Open record: β-glycosidase (Ssβ-gly) variant E387G, 2-nitrophenyl-β-D-glucopyranoside
β-glycosidase (Taβ-gly)	Thermosphaera aggregans	2-nitrophenyl-β-D-glucopyranoside50 mM sodium phosphate buffer	309	0.2	1576	pH 6.5, 65 °C	Open record: β-glycosidase (Taβ-gly), 2-nitrophenyl-β-D-glucopyranoside
β-glycosidase (Taβ-gly)	Thermosphaera aggregans	2-nitrophenyl-β-D-glucopyranoside	439.8	0.38	1165	pH 6.5, 65 °C	Open record: β-glycosidase (Taβ-gly), 2-nitrophenyl-β-D-glucopyranoside
β-glycosidase (Taβ-gly)variant E386G	Thermosphaera aggregans	2-nitrophenyl-β-D-glucopyranoside	72.9	1.01	72.9	pH 6.5, 65 °C	Open record: β-glycosidase (Taβ-gly) variant E386G, 2-nitrophenyl-β-D-glucopyranoside
β-glycosidase (Taβ-gly)variant E386G	Thermosphaera aggregans	2-nitrophenyl-β-D-glucopyranoside	970	6.6	147.4	pH 3, 65 °C	Open record: β-glycosidase (Taβ-gly) variant E386G, 2-nitrophenyl-β-D-glucopyranoside
β-glycosidase (Taβ-gly)variant E386G	Thermosphaera aggregans	2-nitrophenyl-β-D-glucopyranoside	305.3	3.3	91	pH 4, 65 °C	Open record: β-glycosidase (Taβ-gly) variant E386G, 2-nitrophenyl-β-D-glucopyranoside
β-glycosidase (Taβ-gly)variant E386G	Thermosphaera aggregans	2-nitrophenyl-β-D-glucopyranoside	53.9	3.1	17.2	pH 5, 65 °C	Open record: β-glycosidase (Taβ-gly) variant E386G, 2-nitrophenyl-β-D-glucopyranoside
β-glycosidase (Taβ-gly)variant E386G	Thermosphaera aggregans	2-nitrophenyl-β-D-glucopyranoside	11.6	2.8	4.2	pH 6, 65 °C	Open record: β-glycosidase (Taβ-gly) variant E386G, 2-nitrophenyl-β-D-glucopyranoside
β-glycosidase CelB	Pyrococcus furiosus	2-nitrophenyl-β-D-glucopyranoside50 mM sodium phosphate buffer	1797	0.28	6.48 × 10³	pH 6.5, 65 °C	Open record: β-glycosidase CelB, 2-nitrophenyl-β-D-glucopyranoside
β-glycosidase CelB	Pyrococcus furiosus	2-nitrophenyl-β-D-glucopyranoside	1189	0.22	5351	pH 6.5, 65 °C	Open record: β-glycosidase CelB, 2-nitrophenyl-β-D-glucopyranoside
β-glycosidase CelBvariant E372A	Pyrococcus furiosus	2-nitrophenyl-β-D-glucopyranoside	3.9	1.49	2.6	pH 6.5, 65 °C	Open record: β-glycosidase CelB variant E372A, 2-nitrophenyl-β-D-glucopyranoside
β-glycosidase CelBvariant E372A	Pyrococcus furiosus	2-nitrophenyl-β-D-glucopyranoside	47.6	4.3	10.9	pH 3, 65 °C	Open record: β-glycosidase CelB variant E372A, 2-nitrophenyl-β-D-glucopyranoside
β-glycosidase CelBvariant E372A	Pyrococcus furiosus	2-nitrophenyl-β-D-glucopyranoside	6.7	2.1	3.1	pH 4, 65 °C	Open record: β-glycosidase CelB variant E372A, 2-nitrophenyl-β-D-glucopyranoside
β-glycosidase CelBvariant E372A	Pyrococcus furiosus	2-nitrophenyl-β-D-glucopyranoside	0.45	1.3	0.3	pH 5, 65 °C	Open record: β-glycosidase CelB variant E372A, 2-nitrophenyl-β-D-glucopyranoside