Values CatLog read from this paper
The strength of dehalogenase-substrate hydrogen bonding correlates with the rate of Meisenheimer intermediate formation
PMID 12899635doi:10.1021/bi0347656Open in Browse
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CatLog read 7 records (17 values) from this paper: 4-chlorobenzoyl-CoA dehalogenase (4-CBA-CoA dehalogenase) from Pseudomonas sp..
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 4-chlorobenzoyl-CoA dehalogenase (4-CBA-CoA dehalogenase) | Pseudomonas sp. | 4-chlorobenzoyl-coenzyme A | 0.6 | 0.0037 | 160 | pH 7.5, 25 °C | Open record: 4-chlorobenzoyl-CoA dehalogenase (4-CBA-CoA dehalogenase), 4-chlorobenzoyl-coenzyme A |
| 4-chlorobenzoyl-CoA dehalogenase (4-CBA-CoA dehalogenase)variant A112V | Pseudomonas sp. | 4-chlorobenzoyl-coenzyme A | 0.13 | 0.0066 | 19 | pH 7.5, 25 °C | Open record: 4-chlorobenzoyl-CoA dehalogenase (4-CBA-CoA dehalogenase) variant A112V, 4-chlorobenzoyl-coenzyme A |
| 4-chlorobenzoyl-CoA dehalogenase (4-CBA-CoA dehalogenase)variant F64P | Pseudomonas sp. | 4-chlorobenzoyl-coenzyme A | 7.6 × 10⁻⁶ | — | — | pH 7.5, 25 °C | Open record: 4-chlorobenzoyl-CoA dehalogenase (4-CBA-CoA dehalogenase) variant F64P, 4-chlorobenzoyl-coenzyme A |
| 4-chlorobenzoyl-CoA dehalogenase (4-CBA-CoA dehalogenase)variant G113A | Pseudomonas sp. | 4-chlorobenzoyl-coenzyme A | 0.008 | 0.026 | 0.32 | pH 7.5, 25 °C | Open record: 4-chlorobenzoyl-CoA dehalogenase (4-CBA-CoA dehalogenase) variant G113A, 4-chlorobenzoyl-coenzyme A |
| 4-chlorobenzoyl-CoA dehalogenase (4-CBA-CoA dehalogenase)variant G113N | Pseudomonas sp. | 4-chlorobenzoyl-coenzyme A | 4.6 × 10⁻⁵ | — | — | pH 7.5, 25 °C | Open record: 4-chlorobenzoyl-CoA dehalogenase (4-CBA-CoA dehalogenase) variant G113N, 4-chlorobenzoyl-coenzyme A |
| 4-chlorobenzoyl-CoA dehalogenase (4-CBA-CoA dehalogenase)variant G113S | Pseudomonas sp. | 4-chlorobenzoyl-coenzyme A | 4.5 × 10⁻⁴ | 0.062 | 0.0065 | pH 7.5, 25 °C | Open record: 4-chlorobenzoyl-CoA dehalogenase (4-CBA-CoA dehalogenase) variant G113S, 4-chlorobenzoyl-coenzyme A |
| 4-chlorobenzoyl-CoA dehalogenase (4-CBA-CoA dehalogenase)variant Y65D | Pseudomonas sp. | 4-chlorobenzoyl-coenzyme A | 0.44 | 0.011 | 39 | pH 7.5, 25 °C | Open record: 4-chlorobenzoyl-CoA dehalogenase (4-CBA-CoA dehalogenase) variant Y65D, 4-chlorobenzoyl-coenzyme A |