Values CatLog read from this paper
Catalytic mechanism of dichloromethane dehalogenase from Methylophilus sp. strain DM11
PMID 12974641doi:10.1021/bi035016rOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (32 values) from this paper: dichloromethane dehalogenase (DM11) from Methylophilus sp..
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| dichloromethane dehalogenase (DM11) | Methylophilus sp. | bromochloromethane | 1 | 0.00162 | 625 | pH 8, 25 °C | Open record: dichloromethane dehalogenase (DM11), bromochloromethane |
| dichloromethane dehalogenase (DM11) | Methylophilus sp. | bromoethane | 0.14 | 0.33 | 0.42 | pH 8, 25 °C | Open record: dichloromethane dehalogenase (DM11), bromoethane |
| dichloromethane dehalogenase (DM11) | Methylophilus sp. | bromoethane | 0.13 | — | — | pH 8 | Open record: dichloromethane dehalogenase (DM11), bromoethane |
| dichloromethane dehalogenase (DM11) | Methylophilus sp. | chloroethane | 0.1 | 0.33 | 0.3 | pH 8, 25 °C | Open record: dichloromethane dehalogenase (DM11), chloroethane |
| dichloromethane dehalogenase (DM11) | Methylophilus sp. | chlorofluoromethane | 0.009 | 0.053 | 0.17 | pH 8, 25 °C | Open record: dichloromethane dehalogenase (DM11), chlorofluoromethane |
| dichloromethane dehalogenase (DM11) | Methylophilus sp. | chloroiodomethaneNADH formation at 340 nm; 10 nM DM11 | 1.03 | 0.002 | 540 | pH 8, 25 °C | Open record: dichloromethane dehalogenase (DM11), chloroiodomethane |
| dichloromethane dehalogenase (DM11) | Methylophilus sp. | chloroiodomethaneiodide release assay at 226 nm; 25 nM DM11 | 1.4 | — | — | pH 8, 25 °C | Open record: dichloromethane dehalogenase (DM11), chloroiodomethane |
| dichloromethane dehalogenase (DM11) | Methylophilus sp. | dibromomethane | 3.18 | 0.0031 | 1 × 10³ | pH 8, 25 °C | Open record: dichloromethane dehalogenase (DM11), dibromomethane |
| dichloromethane dehalogenase (DM11) | Methylophilus sp. | dichloromethane | 1.51 | 0.031 | 49 | pH 8, 25 °C | Open record: dichloromethane dehalogenase (DM11), dichloromethane |
| dichloromethane dehalogenase (DM11) | Methylophilus sp. | diiodomethaneNADH formation at 340 nm; 10 nM DM11 | 1.31 | 0.0017 | 770 | pH 8, 25 °C | Open record: dichloromethane dehalogenase (DM11), diiodomethane |
| dichloromethane dehalogenase (DM11) | Methylophilus sp. | diiodomethaneiodide release assay at 226 nm; 25 nM DM11 | 1.5 | — | — | pH 8, 25 °C | Open record: dichloromethane dehalogenase (DM11), diiodomethane |
| dichloromethane dehalogenase (DM11) | Methylophilus sp. | iodoethane | 0.15 | 0.039 | 38 | pH 8, 25 °C | Open record: dichloromethane dehalogenase (DM11), iodoethane |
| dichloromethane dehalogenase (DM11) | Methylophilus sp. | reduced glutathione | 1.5 | 0.099 | 15 calc. | pH 8, 25 °C | Open record: dichloromethane dehalogenase (DM11), reduced glutathione |