Values CatLog read from this paper
Purification, characterization, cloning, and expression of a glutamic acid-specific protease from Bacillus licheniformis ATCC 14580
PMID 1429718doi:10.1016/s0021-9258(18)35906-4Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (28 values) from this paper: BLase (glutamic acid-specific protease) from Bacillus licheniformis.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 2 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| BLase (glutamic acid-specific protease) | Bacillus licheniformis | benzyloxycarbonyl-Ala-Ala-Glu p-nitroanilide | 40.9 | 3.5 | 12 calc. | pH 8, 37 °C | Open record: BLase (glutamic acid-specific protease), benzyloxycarbonyl-Ala-Ala-Glu p-nitroanilide |
| BLase (glutamic acid-specific protease) | Bacillus licheniformis | benzyloxycarbonyl-Ala-Glu p-nitroanilide | 5.9 | 4.2 | 1.4 | pH 8, 37 °C | Open record: BLase (glutamic acid-specific protease), benzyloxycarbonyl-Ala-Glu p-nitroanilide |
| BLase (glutamic acid-specific protease) | Bacillus licheniformis | benzyloxycarbonyl-Ala-Leu-Glu p-nitroanilide | 131 | 1.5 | 87.3 | pH 8, 37 °C | Open record: BLase (glutamic acid-specific protease), benzyloxycarbonyl-Ala-Leu-Glu p-nitroanilide |
| BLase (glutamic acid-specific protease) | Bacillus licheniformis | benzyloxycarbonyl-Glu p-nitroanilide | 0.28 | 1.8 | 0.15 | pH 8, 37 °C | Open record: BLase (glutamic acid-specific protease), benzyloxycarbonyl-Glu p-nitroanilide |
| BLase (glutamic acid-specific protease) | Bacillus licheniformis | benzyloxycarbonyl-Leu-Glu p-nitroanilide | 22.6 | 5.5 | 4.1 | pH 8, 37 °C | Open record: BLase (glutamic acid-specific protease), benzyloxycarbonyl-Leu-Glu p-nitroanilide |
| BLase (glutamic acid-specific protease) | Bacillus licheniformis | benzyloxycarbonyl-Leu-Leu-Glu p-nitroanilide | 50.8 | 2.8 | 18.1 | pH 8, 37 °C | Open record: BLase (glutamic acid-specific protease), benzyloxycarbonyl-Leu-Leu-Glu p-nitroanilide |
| BLase (glutamic acid-specific protease) | Bacillus licheniformis | benzyloxycarbonyl-Phe-Leu-Glu p-nitroanilide | 40.9 | 3.9 | 10.5 | pH 8, 37 °C | Open record: BLase (glutamic acid-specific protease), benzyloxycarbonyl-Phe-Leu-Glu p-nitroanilide |
| BLase (glutamic acid-specific protease) | Bacillus licheniformis | benzyloxycarbonyl-Phe-Leu-Glu p-nitroanilide | 7.9 | 6.2 | 1.3 calc. | Open record: BLase (glutamic acid-specific protease), benzyloxycarbonyl-Phe-Leu-Glu p-nitroanilide | |
| BLase (glutamic acid-specific protease) | Bacillus licheniformis | t-butoxycarbonyl-Ala-Ala-Asp p-nitroanilide | 0.16 | 11.6 | 0.014 | pH 8, 37 °C | Open record: BLase (glutamic acid-specific protease), t-butoxycarbonyl-Ala-Ala-Asp p-nitroanilide |
| BLase (glutamic acid-specific protease) | Bacillus licheniformis | t-butoxycarbonyl-Ala-Ala-Glu p-nitroanilide | 11.9 | 4.9 | 2.4 | pH 8, 37 °C | Open record: BLase (glutamic acid-specific protease), t-butoxycarbonyl-Ala-Ala-Glu p-nitroanilide |