Values CatLog read from this paper
Engineering and characterization of a NADPH-utilizing cytochrome b5 reductase
PMID 14503867doi:10.1021/bi034819bOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 18 records (54 values) from this paper: cytochrome b5 reductase (CB5R), soluble diaphorase domain from Rattus norvegicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cytochrome b5 reductase (CB5R), soluble diaphorase domain | Rattus norvegicus | NADPH | 33 | 0.924 | 36 | pH 7, 25 °C | Open record: cytochrome b5 reductase (CB5R), soluble diaphorase domain, NADPH |
| cytochrome b5 reductase (CB5R), soluble diaphorase domain | Rattus norvegicus | reduced nicotinamide adenine dinucleotide | 800 | 0.006 | 1.4 × 10⁵ | pH 7, 25 °C | Open record: cytochrome b5 reductase (CB5R), soluble diaphorase domain, reduced nicotinamide adenine dinucleotide |
| cytochrome b5 reductase (CB5R), soluble diaphorase domainvariant D239E | Rattus norvegicus | NADPH | 5.2 | 0.094 | 58 | pH 7, 25 °C | Open record: cytochrome b5 reductase (CB5R), soluble diaphorase domain variant D239E, NADPH |
| cytochrome b5 reductase (CB5R), soluble diaphorase domainvariant D239E | Rattus norvegicus | reduced nicotinamide adenine dinucleotide | 517 | 0.0062 | 8.6 × 10⁴ | pH 7, 25 °C | Open record: cytochrome b5 reductase (CB5R), soluble diaphorase domain variant D239E, reduced nicotinamide adenine dinucleotide |
| cytochrome b5 reductase (CB5R), soluble diaphorase domainvariant D239S | Rattus norvegicus | NADPH | 217 | 0.268 | 820 | pH 7, 25 °C | Open record: cytochrome b5 reductase (CB5R), soluble diaphorase domain variant D239S, NADPH |
| cytochrome b5 reductase (CB5R), soluble diaphorase domainvariant D239S | Rattus norvegicus | reduced nicotinamide adenine dinucleotide | 433 | 0.017 | 2.6 × 10⁴ | pH 7, 25 °C | Open record: cytochrome b5 reductase (CB5R), soluble diaphorase domain variant D239S, reduced nicotinamide adenine dinucleotide |
| cytochrome b5 reductase (CB5R), soluble diaphorase domainvariant D239S/F251R | Rattus norvegicus | NADPH | 200 | 0.022 | 9.3 × 10³ | pH 7, 25 °C | Open record: cytochrome b5 reductase (CB5R), soluble diaphorase domain variant D239S/F251R, NADPH |
| cytochrome b5 reductase (CB5R), soluble diaphorase domainvariant D239S/F251R | Rattus norvegicus | reduced nicotinamide adenine dinucleotide | 250 | 0.224 | 1.13 × 10³ | pH 7, 25 °C | Open record: cytochrome b5 reductase (CB5R), soluble diaphorase domain variant D239S/F251R, reduced nicotinamide adenine dinucleotide |
| cytochrome b5 reductase (CB5R), soluble diaphorase domainvariant D239S/F251Y | Rattus norvegicus | NADPH | 417 | 0.025 | 1.7 × 10⁴ | pH 7, 25 °C | Open record: cytochrome b5 reductase (CB5R), soluble diaphorase domain variant D239S/F251Y, NADPH |
| cytochrome b5 reductase (CB5R), soluble diaphorase domainvariant D239S/F251Y | Rattus norvegicus | reduced nicotinamide adenine dinucleotide | 467 | 0.0053 | 9.2 × 10⁴ | pH 7, 25 °C | Open record: cytochrome b5 reductase (CB5R), soluble diaphorase domain variant D239S/F251Y, reduced nicotinamide adenine dinucleotide |
| cytochrome b5 reductase (CB5R), soluble diaphorase domainvariant D239T | Rattus norvegicus | NADPH | 267 | 0.0091 | 3 × 10⁴ | pH 7, 25 °C | Open record: cytochrome b5 reductase (CB5R), soluble diaphorase domain variant D239T, NADPH |
| cytochrome b5 reductase (CB5R), soluble diaphorase domainvariant D239T | Rattus norvegicus | reduced nicotinamide adenine dinucleotide | 333 | 0.119 | 2.87 × 10³ | pH 7, 25 °C | Open record: cytochrome b5 reductase (CB5R), soluble diaphorase domain variant D239T, reduced nicotinamide adenine dinucleotide |
| cytochrome b5 reductase (CB5R), soluble diaphorase domainvariant D239T/F251R | Rattus norvegicus | NADPH | 550 | 0.044 | 1.3 × 10⁴ | pH 7, 25 °C | Open record: cytochrome b5 reductase (CB5R), soluble diaphorase domain variant D239T/F251R, NADPH |
| cytochrome b5 reductase (CB5R), soluble diaphorase domainvariant D239T/F251R | Rattus norvegicus | reduced nicotinamide adenine dinucleotide | 717 | 0.512 | 1.4 × 10³ | pH 7, 25 °C | Open record: cytochrome b5 reductase (CB5R), soluble diaphorase domain variant D239T/F251R, reduced nicotinamide adenine dinucleotide |
| cytochrome b5 reductase (CB5R), soluble diaphorase domainvariant F251R | Rattus norvegicus | NADPH | 50 | 0.138 | 370 | pH 7, 25 °C | Open record: cytochrome b5 reductase (CB5R), soluble diaphorase domain variant F251R, NADPH |
| cytochrome b5 reductase (CB5R), soluble diaphorase domainvariant F251R | Rattus norvegicus | reduced nicotinamide adenine dinucleotide | 500 | 0.012 | 4.3 × 10⁴ | pH 7, 25 °C | Open record: cytochrome b5 reductase (CB5R), soluble diaphorase domain variant F251R, reduced nicotinamide adenine dinucleotide |
| cytochrome b5 reductase (CB5R), soluble diaphorase domainvariant F251Y | Rattus norvegicus | NADPH | 50 | 0.617 | 81 | pH 7, 25 °C | Open record: cytochrome b5 reductase (CB5R), soluble diaphorase domain variant F251Y, NADPH |
| cytochrome b5 reductase (CB5R), soluble diaphorase domainvariant F251Y | Rattus norvegicus | reduced nicotinamide adenine dinucleotide | 367 | 0.0031 | 1.3 × 10⁵ | pH 7, 25 °C | Open record: cytochrome b5 reductase (CB5R), soluble diaphorase domain variant F251Y, reduced nicotinamide adenine dinucleotide |