Values CatLog read from this paper
Relaxing the nicotinamide cofactor specificity of phosphite dehydrogenase by rational design
PMID 14529270doi:10.1021/bi035018bOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (32 values) from this paper: phosphite dehydrogenase (PTDH) from Pseudomonas stutzeri.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| phosphite dehydrogenase (PTDH) | Pseudomonas stutzeri | nicotinamide adenine dinucleotide | 2.93 | 0.053 | 55 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH), nicotinamide adenine dinucleotide |
| phosphite dehydrogenase (PTDH) | Pseudomonas stutzeri | nicotinamide adenine dinucleotide phosphate | 1.41 | 2.51 | 0.562 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH), nicotinamide adenine dinucleotide phosphate |
| phosphite dehydrogenase (PTDH) | Pseudomonas stutzeri | phosphitecosubstrate NAD+ | — | 0.047 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH), phosphite |
| phosphite dehydrogenase (PTDH) | Pseudomonas stutzeri | phosphitecosubstrate NADP+ | — | 1.88 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH), phosphite |
| phosphite dehydrogenase (PTDH)variant A176R | Pseudomonas stutzeri | nicotinamide adenine dinucleotide | 4.28 | 0.06 | 72 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant A176R, nicotinamide adenine dinucleotide |
| phosphite dehydrogenase (PTDH)variant A176R | Pseudomonas stutzeri | nicotinamide adenine dinucleotide phosphate | 2.18 | 0.077 | 28 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant A176R, nicotinamide adenine dinucleotide phosphate |
| phosphite dehydrogenase (PTDH)variant A176R | Pseudomonas stutzeri | phosphitecosubstrate NAD+ | — | 0.156 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant A176R, phosphite |
| phosphite dehydrogenase (PTDH)variant A176R | Pseudomonas stutzeri | phosphitecosubstrate NADP+ | — | 0.14 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant A176R, phosphite |
| phosphite dehydrogenase (PTDH)variant E175A | Pseudomonas stutzeri | nicotinamide adenine dinucleotide | 3.5 | 0.016 | 218 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant E175A, nicotinamide adenine dinucleotide |
| phosphite dehydrogenase (PTDH)variant E175A | Pseudomonas stutzeri | nicotinamide adenine dinucleotide phosphate | 2.18 | 0.144 | 15.2 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant E175A, nicotinamide adenine dinucleotide phosphate |
| phosphite dehydrogenase (PTDH)variant E175A | Pseudomonas stutzeri | phosphitecosubstrate NAD+ | — | 0.023 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant E175A, phosphite |
| phosphite dehydrogenase (PTDH)variant E175A | Pseudomonas stutzeri | phosphitecosubstrate NADP+ | — | 0.138 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant E175A, phosphite |
| phosphite dehydrogenase (PTDH)variant E175A/A176R | Pseudomonas stutzeri | nicotinamide adenine dinucleotide | 3.94 | 0.02 | 197 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant E175A/A176R, nicotinamide adenine dinucleotide |
| phosphite dehydrogenase (PTDH)variant E175A/A176R | Pseudomonas stutzeri | nicotinamide adenine dinucleotide phosphate | 1.9 | 0.0035 | 543 | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant E175A/A176R, nicotinamide adenine dinucleotide phosphate |
| phosphite dehydrogenase (PTDH)variant E175A/A176R | Pseudomonas stutzeri | phosphitecosubstrate NAD+ | — | 0.061 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant E175A/A176R, phosphite |
| phosphite dehydrogenase (PTDH)variant E175A/A176R | Pseudomonas stutzeri | phosphitecosubstrate NADP+ | — | 0.021 | — | pH 7.25, 25 °C | Open record: phosphite dehydrogenase (PTDH) variant E175A/A176R, phosphite |