Values CatLog read from this paper
Evaluation by mutagenesis of the importance of 3 arginines in alpha, beta, and gamma subunits of human NAD-dependent isocitrate dehydrogenase
PMID 14555658doi:10.1074/jbc.m306178200Open in Browse
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CatLog read 11 records (11 values) from this paper: NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits) from Homo sapiens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits) | Homo sapiens | isocitrate1 mM MnSO4; no ADP | — | 2.2 | — | pH 7.2, 25 °C | Open record: NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits), isocitrate |
| NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits) | Homo sapiens | isocitrate1.65 mM MnSO4; with 1 mM ADP (allosteric activator) | — | 0.32 | — | pH 7.2, 25 °C | Open record: NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits), isocitrate |
| NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits) | Homo sapiens | NAD+ | — | 0.073 | — | pH 7.2, 25 °C | Open record: NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits), NAD+ |
| NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits)variant β-R99Q | Homo sapiens | isocitrate1 mM MnSO4; no ADP | — | 0.9 | — | pH 7.2, 25 °C | Open record: NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits) variant β-R99Q, isocitrate |
| NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits)variant β-R99Q | Homo sapiens | isocitrate1.65 mM MnSO4; with 1 mM ADP (allosteric activator) | — | 0.77 | — | pH 7.2, 25 °C | Open record: NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits) variant β-R99Q, isocitrate |
| NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits)variant β-R99Q | Homo sapiens | NAD+ | — | 0.047 | — | pH 7.2, 25 °C | Open record: NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits) variant β-R99Q, NAD+ |
| NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits)variant γ-R97Q | Homo sapiens | isocitrate1 mM MnSO4; 1 mM NAD; no ADP | — | 2 | — | pH 7.2, 25 °C | Open record: NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits) variant γ-R97Q, isocitrate |
| NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits)variant γ-R97Q | Homo sapiens | isocitrate1.65 mM MnSO4; 1 mM NAD | — | 1.8 | — | pH 7.2, 25 °C | Open record: NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits) variant γ-R97Q, isocitrate |
| NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits)variant γ-R97Q | Homo sapiens | isocitrate1 mM MnSO4; 5 mM NAD; no ADP | — | 1.8 | — | pH 7.2, 25 °C | Open record: NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits) variant γ-R97Q, isocitrate |
| NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits)variant γ-R97Q | Homo sapiens | isocitrate1.65 mM MnSO4; 5 mM NAD | — | 1.49 | — | pH 7.2, 25 °C | Open record: NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits) variant γ-R97Q, isocitrate |
| NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits)variant γ-R97Q | Homo sapiens | NAD+ | — | 0.82 | — | pH 7.2, 25 °C | Open record: NAD-dependent isocitrate dehydrogenase (α2βγ; α, β2 isoform, γ subunits) variant γ-R97Q, NAD+ |