Values CatLog read from this paper
Mutagenesis of putative catalytic and regulatory residues of Streptomyces chromofuscus phospholipase D differentially modifies phosphatase and phosphodiesterase activities
PMID 14557260doi:10.1074/jbc.m310252200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 13 records (36 values) from this paper: phospholipase D (scPLD) from Streptomyces chromofuscus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| phospholipase D (scPLD) | Streptomyces chromofuscus | bis(p-nitrophenyl) phosphate | 0.67 | 0.17 | 3.94 | pH 8 | Open record: phospholipase D (scPLD), bis(p-nitrophenyl) phosphate |
| phospholipase D (scPLD) | Streptomyces chromofuscus | p-nitrophenyl phosphate | 0.7 | 2.5 | 0.28 | pH 8 | Open record: phospholipase D (scPLD), p-nitrophenyl phosphate |
| phospholipase D (scPLD) | Streptomyces chromofuscus | p-nitrophenylphosphocholine | 1.9 | 7.1 | 0.271 | pH 8 | Open record: phospholipase D (scPLD), p-nitrophenylphosphocholine |
| phospholipase D (scPLD)variant D389A | Streptomyces chromofuscus | bis(p-nitrophenyl) phosphate | 0.01 | 0.41 | 0.0244 | pH 8 | Open record: phospholipase D (scPLD) variant D389A, bis(p-nitrophenyl) phosphate |
| phospholipase D (scPLD)variant D389A | Streptomyces chromofuscus | p-nitrophenyl phosphate | 0.058 | 0.33 | 0.176 | pH 8 | Open record: phospholipase D (scPLD) variant D389A, p-nitrophenyl phosphate |
| phospholipase D (scPLD)variant E213A | Streptomyces chromofuscus | bis(p-nitrophenyl) phosphate | 0.003 | 0.23 | 0.013 | pH 8 | Open record: phospholipase D (scPLD) variant E213A, bis(p-nitrophenyl) phosphate |
| phospholipase D (scPLD)variant E213A | Streptomyces chromofuscus | diheptanoyl phosphatidylcholine | 0.09 | — | — | 25 °C | Open record: phospholipase D (scPLD) variant E213A, diheptanoyl phosphatidylcholine |
| phospholipase D (scPLD)variant E213A | Streptomyces chromofuscus | p-nitrophenyl phosphate | 0.015 | 2.6 | 0.0057 | pH 8 | Open record: phospholipase D (scPLD) variant E213A, p-nitrophenyl phosphate |
| phospholipase D (scPLD)variant E213A | Streptomyces chromofuscus | p-nitrophenylphosphocholine | 0.04 | 3.1 | 0.0118 | pH 8 | Open record: phospholipase D (scPLD) variant E213A, p-nitrophenylphosphocholine |
| phospholipase D (scPLD)variant H212A | Streptomyces chromofuscus | bis(p-nitrophenyl) phosphate | 0.05 | 7.8 | 0.0064 | pH 8 | Open record: phospholipase D (scPLD) variant H212A, bis(p-nitrophenyl) phosphate |
| phospholipase D (scPLD)variant H212A | Streptomyces chromofuscus | p-nitrophenyl phosphate | 0.018 | 23.2 | 7.7 × 10⁻⁴ | pH 8 | Open record: phospholipase D (scPLD) variant H212A, p-nitrophenyl phosphate |
| phospholipase D (scPLD)variant H212A | Streptomyces chromofuscus | p-nitrophenylphosphocholine | 0.39 | 182 | 0.002 | pH 8 | Open record: phospholipase D (scPLD) variant H212A, p-nitrophenylphosphocholine |
| phospholipase D (scPLD)variant H226A | Streptomyces chromofuscus | p-nitrophenylphosphocholine | 1.6 | 17 | 0.094 calc. | pH 8 | Open record: phospholipase D (scPLD) variant H226A, p-nitrophenylphosphocholine |