Values CatLog read from this paper
Interactions of a family 18 chitinase with the designed inhibitor HM508 and its degradation product, chitobiono-delta-lactone
PMID 14597613doi:10.1074/jbc.m310057200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 4 records (8 values) from this paper: chitinase B (ChiB) from Serratia marcescens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| chitinase B (ChiB) | Serratia marcescens | 4-methylumbelliferyl N,N'-diacetyl-beta-D-chitobioside | 10 | 0.024 | 420 calc. | pH 6.3, 37 °C | Open record: chitinase B (ChiB), 4-methylumbelliferyl N,N'-diacetyl-beta-D-chitobioside |
| chitinase B (ChiB)variant D142N | Serratia marcescens | 4-methylumbelliferyl N,N'-diacetyl-beta-D-chitobioside | 0.17 | 0.0035 | 49 calc. | pH 6.3, 37 °C | Open record: chitinase B (ChiB) variant D142N, 4-methylumbelliferyl N,N'-diacetyl-beta-D-chitobioside |
| chitinase B (ChiB)variant M212A | Serratia marcescens | 4-methylumbelliferyl N,N'-diacetyl-beta-D-chitobioside | 1.2 | 0.0063 | 190 calc. | pH 6.3, 37 °C | Open record: chitinase B (ChiB) variant M212A, 4-methylumbelliferyl N,N'-diacetyl-beta-D-chitobioside |
| chitinase B (ChiB)variant W220A | Serratia marcescens | 4-methylumbelliferyl N,N'-diacetyl-beta-D-chitobioside | 0.5 | 0.084 | 6.0 calc. | pH 6.3, 37 °C | Open record: chitinase B (ChiB) variant W220A, 4-methylumbelliferyl N,N'-diacetyl-beta-D-chitobioside |