Values CatLog read from this paper
A substrate-induced switch in the reaction mechanism of a thermophilic esterase: kinetic evidences and structural basis
PMID 14617621doi:10.1074/jbc.m307738200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 22 records (66 values) from this paper: esterase 2 (EST2) from Alicyclobacillus acidocaldarius.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| esterase 2 (EST2) | Alicyclobacillus acidocaldarius | p-nitrophenyl dodecanoate | 340 | 0.0043 | 7.8 × 10⁴ | pH 7.1, 70 °C | Open record: esterase 2 (EST2), p-nitrophenyl dodecanoate |
| esterase 2 (EST2) | Alicyclobacillus acidocaldarius | p-nitrophenyl dodecanoate | 30 | 0.003 | 1 × 10⁴ | pH 7.1, 25 °C | Open record: esterase 2 (EST2), p-nitrophenyl dodecanoate |
| esterase 2 (EST2) | Alicyclobacillus acidocaldarius | p-nitrophenyl dodecanoate | 37 | 0.0028 | 1.2 × 10⁴ | pH 7.1, 30 °C | Open record: esterase 2 (EST2), p-nitrophenyl dodecanoate |
| esterase 2 (EST2) | Alicyclobacillus acidocaldarius | p-nitrophenyl dodecanoate | 45 | 0.0035 | 1.4 × 10⁴ | pH 7.1, 35 °C | Open record: esterase 2 (EST2), p-nitrophenyl dodecanoate |
| esterase 2 (EST2) | Alicyclobacillus acidocaldarius | p-nitrophenyl dodecanoate | 85 | 0.0039 | 2.1 × 10⁴ | pH 7.1, 45 °C | Open record: esterase 2 (EST2), p-nitrophenyl dodecanoate |
| esterase 2 (EST2) | Alicyclobacillus acidocaldarius | p-nitrophenyl dodecanoate | 118 | 0.004 | 2.9 × 10⁴ | pH 7.1, 55 °C | Open record: esterase 2 (EST2), p-nitrophenyl dodecanoate |
| esterase 2 (EST2) | Alicyclobacillus acidocaldarius | p-nitrophenyl dodecanoate | 205 | 0.0041 | 5 × 10⁴ | pH 7.1, 65 °C | Open record: esterase 2 (EST2), p-nitrophenyl dodecanoate |
| esterase 2 (EST2) | Alicyclobacillus acidocaldarius | p-nitrophenyl dodecanoate | 333 | 0.0046 | 7.2 × 10⁴ | pH 7.1, 72.5 °C | Open record: esterase 2 (EST2), p-nitrophenyl dodecanoate |
| esterase 2 (EST2) | Alicyclobacillus acidocaldarius | p-nitrophenyl dodecanoate | 327 | 0.0047 | 7 × 10⁴ | pH 7.1, 75 °C | Open record: esterase 2 (EST2), p-nitrophenyl dodecanoate |
| esterase 2 (EST2) | Alicyclobacillus acidocaldarius | p-nitrophenyl dodecanoate | 333 | 0.0049 | 6.8 × 10⁴ | pH 7.1, 77.5 °C | Open record: esterase 2 (EST2), p-nitrophenyl dodecanoate |
| esterase 2 (EST2)variant M211S | Alicyclobacillus acidocaldarius | p-nitrophenyl dodecanoate | 350 | 0.0024 | 1.46 × 10⁵ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211S, p-nitrophenyl dodecanoate |
| esterase 2 (EST2)variant M211S/R215L | Alicyclobacillus acidocaldarius | p-nitrophenyl dodecanoate | 940 | 0.0034 | 2.76 × 10⁵ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant M211S/R215L, p-nitrophenyl dodecanoate |
| esterase 2 (EST2)variant M211S/R215L | Alicyclobacillus acidocaldarius | p-nitrophenyl dodecanoate | 76 | 0.0023 | 3.3 × 10⁴ | pH 7.1, 25 °C | Open record: esterase 2 (EST2) variant M211S/R215L, p-nitrophenyl dodecanoate |
| esterase 2 (EST2)variant M211S/R215L | Alicyclobacillus acidocaldarius | p-nitrophenyl dodecanoate | 103 | 0.0025 | 4.1 × 10⁴ | pH 7.1, 30 °C | Open record: esterase 2 (EST2) variant M211S/R215L, p-nitrophenyl dodecanoate |
| esterase 2 (EST2)variant M211S/R215L | Alicyclobacillus acidocaldarius | p-nitrophenyl dodecanoate | 138 | 0.003 | 4.6 × 10⁴ | pH 7.1, 35 °C | Open record: esterase 2 (EST2) variant M211S/R215L, p-nitrophenyl dodecanoate |
| esterase 2 (EST2)variant M211S/R215L | Alicyclobacillus acidocaldarius | p-nitrophenyl dodecanoate | 212 | 0.0031 | 6.8 × 10⁴ | pH 7.1, 45 °C | Open record: esterase 2 (EST2) variant M211S/R215L, p-nitrophenyl dodecanoate |
| esterase 2 (EST2)variant M211S/R215L | Alicyclobacillus acidocaldarius | p-nitrophenyl dodecanoate | 452 | 0.0032 | 1.41 × 10⁵ | pH 7.1, 55 °C | Open record: esterase 2 (EST2) variant M211S/R215L, p-nitrophenyl dodecanoate |
| esterase 2 (EST2)variant M211S/R215L | Alicyclobacillus acidocaldarius | p-nitrophenyl dodecanoate | 842 | 0.0032 | 2.7 × 10⁵ | pH 7.1, 65 °C | Open record: esterase 2 (EST2) variant M211S/R215L, p-nitrophenyl dodecanoate |
| esterase 2 (EST2)variant M211S/R215L | Alicyclobacillus acidocaldarius | p-nitrophenyl dodecanoate | 950 | 0.0036 | 2.64 × 10⁵ | pH 7.1, 72.5 °C | Open record: esterase 2 (EST2) variant M211S/R215L, p-nitrophenyl dodecanoate |
| esterase 2 (EST2)variant M211S/R215L | Alicyclobacillus acidocaldarius | p-nitrophenyl dodecanoate | 825 | 0.0038 | 2.17 × 10⁵ | pH 7.1, 75 °C | Open record: esterase 2 (EST2) variant M211S/R215L, p-nitrophenyl dodecanoate |
| esterase 2 (EST2)variant M211S/R215L | Alicyclobacillus acidocaldarius | p-nitrophenyl dodecanoate | 810 | 0.004 | 2.03 × 10⁵ | pH 7.1, 77.5 °C | Open record: esterase 2 (EST2) variant M211S/R215L, p-nitrophenyl dodecanoate |
| esterase 2 (EST2)variant R215L | Alicyclobacillus acidocaldarius | p-nitrophenyl dodecanoate | 705 | 0.0046 | 1.53 × 10⁵ | pH 7.1, 70 °C | Open record: esterase 2 (EST2) variant R215L, p-nitrophenyl dodecanoate |