Values CatLog read from this paper
Roles of individual enzyme-substrate interactions by alpha-1,3-galactosyltransferase in catalysis and specificity
PMID 14621997doi:10.1021/bi035430rOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (24 values) from this paper: UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT) from Bos taurus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT) | Bos taurus | lactose | 6.4 | 19.9 | 0.32 calc. | 37 °C | Open record: UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT), lactose |
| UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT) | Bos taurus | UDP-galactoseboth substrates varied (UDP-Gal generally 0.1-2.0 mM) | — | 0.43 | — | 37 °C | Open record: UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT), UDP-galactose |
| UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT) | Bos taurus | UDP-galactoseUDP-Gal varied (generally 0.1-1.0 mM) | 0.016 | 0.1 | 0.16 | 37 °C | Open record: UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT), UDP-galactose |
| UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT)variant E317Q | Bos taurus | lactose | 0.0027 | 233 | 1.2 × 10⁻⁵ calc. | 37 °C | Open record: UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT) variant E317Q, lactose |
| UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT)variant E317Q | Bos taurus | UDP-galactoseUDP-Gal varied at fixed 25 mM lactose; cosubstrate lactose | — | 0.13 | — | 37 °C | Open record: UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT) variant E317Q, UDP-galactose |
| UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT)variant E317Q | Bos taurus | UDP-galactoseUDP-Gal varied (generally 0.1-1.0 mM) | 1.3 × 10⁻⁴ | 0.16 | 6.9 × 10⁻⁴ | 37 °C | Open record: UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT) variant E317Q, UDP-galactose |
| UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT)variant H280Q | Bos taurus | lactose | 0.77 | 33.6 | 0.023 calc. | 37 °C | Open record: UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT) variant H280Q, lactose |
| UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT)variant H280Q | Bos taurus | UDP-galactose | — | 1.02 | — | 37 °C | Open record: UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT) variant H280Q, UDP-galactose |
| UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT)variant Q247E | Bos taurus | lactose | 0.14 | 20.2 | 0.0069 calc. | 37 °C | Open record: UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT) variant Q247E, lactose |
| UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT)variant Q247E | Bos taurus | UDP-galactoseUDP-Gal varied at fixed 25 mM lactose; cosubstrate lactose | — | 0.66 | — | 37 °C | Open record: UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT) variant Q247E, UDP-galactose |
| UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT)variant Q247E | Bos taurus | UDP-galactoseUDP-Gal varied (generally 0.1-1.0 mM) | 0.011 | 0.15 | 0.073 | 37 °C | Open record: UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT) variant Q247E, UDP-galactose |
| UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT)variant R365K | Bos taurus | UDP-galactose | 0.0014 | 0.14 | 0.01 | 37 °C | Open record: UDP-galactose β-galactosyl α-1,3-galactosyltransferase (α3GT) variant R365K, UDP-galactose |