CatLogenzyme kinetics catalog

Values CatLog read from this paper

Kinetic analysis of phenylalanine dehydrogenase mutants designed for aliphatic amino acid dehydrogenase activity with guidance from homology-based modelling

PMID 14622249doi:10.1046/j.1432-1033.2003.03852.xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 48 records (136 values) from this paper: phenylalanine dehydrogenase (PheDH) from Bacillus sphaericus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.4.1.20

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
phenylalanine dehydrogenase (PheDH)variant G124ABacillus sphaericus2-oxohexanoate642.130pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124ABacillus sphaericus2-oxopentanoate532.521pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124ABacillus sphaericus3-methyl-2-oxobutanoate51317pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124ABacillus sphaericus4-(methylthio)-2-oxobutanoate382.416pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124ABacillus sphaericus4-hydroxyphenylpyruvate——0.91pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124ABacillus sphaericus4-methyl-2-oxopentanoate250.6340pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124ABacillus sphaericusDL-3-methyl-2-oxopentanoate161.312pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124ABacillus sphaericusL-Isoleucine1.10.1110pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124ABacillus sphaericusL-Leucine10.05718pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124ABacillus sphaericusL-Methionine1.40.731.9pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124ABacillus sphaericusL-Norleucine1.60.09617pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124ABacillus sphaericusL-Norvaline1.50.197.9pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124ABacillus sphaericusL-Phenylalanine4.28.10.52pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124ABacillus sphaericusL-Tyrosine——0.11pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124ABacillus sphaericusL-Valine0.970.137.5pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124ABacillus sphaericusphenylpyruvate317.34.2pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124A/L307VBacillus sphaericus2-oxohexanoate660.9470pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124A/L307VBacillus sphaericus2-oxopentanoate510.8262pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124A/L307VBacillus sphaericus3-methyl-2-oxobutanoate53227pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124A/L307VBacillus sphaericus4-(methylthio)-2-oxobutanoate521.147pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124A/L307VBacillus sphaericus4-hydroxyphenylpyruvate——0.91pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124A/L307VBacillus sphaericus4-methyl-2-oxopentanoate240.3667pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124A/L307VBacillus sphaericusDL-3-methyl-2-oxopentanoate130.4231pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124A/L307VBacillus sphaericusL-Isoleucine2.30.1121pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124A/L307VBacillus sphaericusL-Leucine1.10.04624pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124A/L307VBacillus sphaericusL-Methionine10.166.3pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124A/L307VBacillus sphaericusL-Norleucine2.20.08825pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124A/L307VBacillus sphaericusL-Norvaline1.40.08117pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124A/L307VBacillus sphaericusL-Phenylalanine5.17.20.71pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124A/L307VBacillus sphaericusL-Tyrosine——0.072pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124A/L307VBacillus sphaericusL-Valine1.90.277pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant G124A/L307VBacillus sphaericusphenylpyruvate464.610pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant L307VBacillus sphaericus2-oxohexanoate170.6925pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant L307VBacillus sphaericus2-oxopentanoate33113pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant L307VBacillus sphaericus3-methyl-2-oxobutanoate25141.8pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant L307VBacillus sphaericus4-(methylthio)-2-oxobutanoate341.523pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant L307VBacillus sphaericus4-hydroxyphenylpyruvate550.23239pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant L307VBacillus sphaericus4-methyl-2-oxopentanoate7.81.45.6pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant L307VBacillus sphaericusDL-3-methyl-2-oxopentanoate108.51.2pH 8.6, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant L307VBacillus sphaericusL-Isoleucine0.270.191.4pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant L307VBacillus sphaericusL-Leucine0.120.0254.8pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant L307VBacillus sphaericusL-Methionine1.20.294.1pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant L307VBacillus sphaericusL-Norleucine1.30.07717pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant L307VBacillus sphaericusL-Norvaline0.910.481.9pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant L307VBacillus sphaericusL-Phenylalanine180.5334pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant L307VBacillus sphaericusL-Tyrosine210.4943pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant L307VBacillus sphaericusL-Valine1.42.20.64pH 10.4, 25 °COpen
phenylalanine dehydrogenase (PheDH)variant L307VBacillus sphaericusphenylpyruvate490.22223pH 8.6, 25 °COpen