Values CatLog read from this paper
X-ray crystallographic and site-directed mutagenesis analysis of the mechanism of Schiff-base formation in phosphonoacetaldehyde hydrolase catalysis
PMID 14670958doi:10.1074/jbc.m312345200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 8 records (24 values) from this paper: phosphonoacetaldehyde hydrolase (phosphonatase) from Bacillus cereus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| phosphonoacetaldehyde hydrolase (phosphonatase) | Bacillus cereus | phosphonoacetaldehyde | 15 | 0.033 | 500 | pH 7.5, 25 °C | Open record: phosphonoacetaldehyde hydrolase (phosphonatase), phosphonoacetaldehyde |
| phosphonoacetaldehyde hydrolase (phosphonatase)variant C22A | Bacillus cereus | phosphonoacetaldehyde | 1.95 | 0.53 | 3.7 | pH 7.5, 25 °C | Open record: phosphonoacetaldehyde hydrolase (phosphonatase) variant C22A, phosphonoacetaldehyde |
| phosphonoacetaldehyde hydrolase (phosphonatase)variant C22S | Bacillus cereus | phosphonoacetaldehyde | 2.26 | 0.033 | 69 | pH 7.5, 25 °C | Open record: phosphonoacetaldehyde hydrolase (phosphonatase) variant C22S, phosphonoacetaldehyde |
| phosphonoacetaldehyde hydrolase (phosphonatase)variant H56A | Bacillus cereus | phosphonoacetaldehyde | 0.075 | 0.145 | 0.52 | pH 7.5, 25 °C | Open record: phosphonoacetaldehyde hydrolase (phosphonatase) variant H56A, phosphonoacetaldehyde |
| phosphonoacetaldehyde hydrolase (phosphonatase)variant M49L | Bacillus cereus | phosphonoacetaldehyde | 0.15 | 5.1 | 0.029 | pH 7.5, 25 °C | Open record: phosphonoacetaldehyde hydrolase (phosphonatase) variant M49L, phosphonoacetaldehyde |
| phosphonoacetaldehyde hydrolase (phosphonatase)variant Y128A | Bacillus cereus | phosphonoacetaldehyde | 0.0773 | 0.035 | 2.2 | pH 7.5, 25 °C | Open record: phosphonoacetaldehyde hydrolase (phosphonatase) variant Y128A, phosphonoacetaldehyde |
| phosphonoacetaldehyde hydrolase (phosphonatase)variant Y128F | Bacillus cereus | phosphonoacetaldehyde | 2.21 | 0.045 | 49 | pH 7.5, 25 °C | Open record: phosphonoacetaldehyde hydrolase (phosphonatase) variant Y128F, phosphonoacetaldehyde |
| phosphonoacetaldehyde hydrolase (phosphonatase)variant Y128F/C22S | Bacillus cereus | phosphonoacetaldehyde | 2.11 | 0.033 | 64 | pH 7.5, 25 °C | Open record: phosphonoacetaldehyde hydrolase (phosphonatase) variant Y128F/C22S, phosphonoacetaldehyde |