CatLogenzyme kinetics catalog

Values CatLog read from this paper

Modulation of activity of NADH oxidase from Thermus thermophilus through change in flexibility in the enzyme active site induced by Hofmeister series anions

PMID 14686918doi:10.1046/j.1432-1033.2003.03900.xOpen in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 34 records (81 values) from this paper: NADH oxidase from Thermus thermophilus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.

EC 1.6.99.3

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme1.10.0085130pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH50 mM potassium phosphate buffer; no added salt; NADH varied6.60.00521.3 × 10³ calc.pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme5.360.0156343pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme10.0305533pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme0.78——pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme0.49——pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme5.10.01565326pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme0.810.0442218pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme0.54——pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme0.27——pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme7.180.02934245pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme6.97——pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme3.86——pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme1.29——pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme7.610.0209364pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme6.750.02171311pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme6.220.02251276pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme5.360.02814191pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme5.010.01325378pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme7.50.01367555pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme6.640.01372484pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme6.430.0134480pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme4.820.01286375pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme4.820.01005580pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme5.360.01894283pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme4.950.02812176pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme2.680.01125240pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme2.190.00723303pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme1.07——pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme0.86——pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme3.320.01367243pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme2.140.01045205pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme1.180.00894132pH 7, 20 °COpen
NADH oxidaseThermus thermophilusNADH5 mM potassium phosphate buffer; 0.12 mM FAD; 120 nM enzyme0.38——pH 7, 20 °COpen