Values CatLog read from this paper
Structure of the D142N mutant of the family 18 chitinase ChiB from Serratia marcescens and its complex with allosamidin
PMID 14726210doi:10.1016/j.bbapap.2003.09.014Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (28 values) from this paper: chitinase B (ChiB) from Serratia marcescens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 14 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| chitinase B (ChiB) | Serratia marcescens | 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside | 13 | 0.031 | 420 calc. | pH 4.2, 37 °C | Open record: chitinase B (ChiB), 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside |
| chitinase B (ChiB) | Serratia marcescens | 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside | 18 | 0.026 | 690 calc. | pH 5.4, 37 °C | Open record: chitinase B (ChiB), 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside |
| chitinase B (ChiB) | Serratia marcescens | 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside | 18 | 0.031 | 580 calc. | pH 6.1, 37 °C | Open record: chitinase B (ChiB), 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside |
| chitinase B (ChiB) | Serratia marcescens | 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside | 17 | 0.038 | 450 calc. | pH 7.2, 37 °C | Open record: chitinase B (ChiB), 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside |
| chitinase B (ChiB) | Serratia marcescens | 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside | 3.5 | 0.025 | 140 calc. | pH 8, 37 °C | Open record: chitinase B (ChiB), 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside |
| chitinase B (ChiB) | Serratia marcescens | 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside | 1.2 | 0.046 | 26 calc. | pH 9, 37 °C | Open record: chitinase B (ChiB), 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside |
| chitinase B (ChiB) | Serratia marcescens | 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside | 0.4 | 0.072 | 5.6 calc. | pH 10, 37 °C | Open record: chitinase B (ChiB), 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside |
| chitinase B (ChiB)variant D142N | Serratia marcescens | 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside | 0.26 | 0.0054 | 48 calc. | pH 4.2, 37 °C | Open record: chitinase B (ChiB) variant D142N, 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside |
| chitinase B (ChiB)variant D142N | Serratia marcescens | 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside | 0.34 | 0.0045 | 76 calc. | pH 5.4, 37 °C | Open record: chitinase B (ChiB) variant D142N, 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside |
| chitinase B (ChiB)variant D142N | Serratia marcescens | 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside | 0.28 | 0.0041 | 68 calc. | pH 6.1, 37 °C | Open record: chitinase B (ChiB) variant D142N, 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside |
| chitinase B (ChiB)variant D142N | Serratia marcescens | 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside | 0.32 | 0.0033 | 97 calc. | pH 7.2, 37 °C | Open record: chitinase B (ChiB) variant D142N, 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside |
| chitinase B (ChiB)variant D142N | Serratia marcescens | 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside | 0.29 | 0.0057 | 51 calc. | pH 8, 37 °C | Open record: chitinase B (ChiB) variant D142N, 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside |
| chitinase B (ChiB)variant D142N | Serratia marcescens | 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside | 0.31 | 0.017 | 18 calc. | pH 9, 37 °C | Open record: chitinase B (ChiB) variant D142N, 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside |
| chitinase B (ChiB)variant D142N | Serratia marcescens | 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside | 0.18 | 0.034 | 5.3 calc. | pH 10, 37 °C | Open record: chitinase B (ChiB) variant D142N, 4-methylumbelliferyl beta-D-N,N'-diacetylchitobioside |