Values CatLog read from this paper

Mutant rat trypsin selectively cleaves tyrosyl peptide bonds

PMID 15003560doi:10.1016/j.ab.2003.12.001Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 23 records (69 values) from this paper: trypsin, alpha-chymotrypsin from Rattus norvegicus, Bos taurus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
alpha-chymotrypsin	Bos taurus	N-succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin	13.08	0.11	119	pH 8, 37 °C	Open record: alpha-chymotrypsin, N-succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin
alpha-chymotrypsin	Bos taurus	N-succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin	0.0412	0.6	0.0687	pH 8, 37 °C	Open record: alpha-chymotrypsin, N-succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin
alpha-chymotrypsin	Bos taurus	N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin	17.3	0.02	787	pH 8, 37 °C	Open record: alpha-chymotrypsin, N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
alpha-chymotrypsin	Bos taurus	N-succinyl-Ala-Ala-Pro-Trp-7-amido-4-methylcoumarin	47.7	0.04	1.29 × 10³	pH 8, 37 °C	Open record: alpha-chymotrypsin, N-succinyl-Ala-Ala-Pro-Trp-7-amido-4-methylcoumarin
alpha-chymotrypsin	Bos taurus	N-succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin	19.7	0.02	855	pH 8, 37 °C	Open record: alpha-chymotrypsin, N-succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin
trypsinTr→Ch[S1+L1+L2] (loop mutant)	Rattus norvegicus	N-succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin	0.0068	0.34	0.0203	pH 8, 37 °C	Open record: trypsin Tr→Ch[S1+L1+L2] (loop mutant), N-succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin
trypsinTr→Ch[S1+L1+L2] (loop mutant)	Rattus norvegicus	N-succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin	0.0267	0.77	0.0347	pH 8, 37 °C	Open record: trypsin Tr→Ch[S1+L1+L2] (loop mutant), N-succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin
trypsinTr→Ch[S1+L1+L2] (loop mutant)	Rattus norvegicus	N-succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin	0.0023	1	0.0023	pH 8, 37 °C	Open record: trypsin Tr→Ch[S1+L1+L2] (loop mutant), N-succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin
trypsinTr→Ch[S1+L1+L2] (loop mutant)	Rattus norvegicus	N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin	0.55	1.52	0.363	pH 8, 37 °C	Open record: trypsin Tr→Ch[S1+L1+L2] (loop mutant), N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
trypsinTr→Ch[S1+L1+L2] (loop mutant)	Rattus norvegicus	N-succinyl-Ala-Ala-Pro-Trp-7-amido-4-methylcoumarin	0.0583	0.42	0.1402	pH 8, 37 °C	Open record: trypsin Tr→Ch[S1+L1+L2] (loop mutant), N-succinyl-Ala-Ala-Pro-Trp-7-amido-4-methylcoumarin
trypsinTr→Ch[S1+L1+L2] (loop mutant)	Rattus norvegicus	N-succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin	0.617	0.67	0.926	pH 8, 37 °C	Open record: trypsin Tr→Ch[S1+L1+L2] (loop mutant), N-succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin
trypsinvariant D189S/TyrGly217-219SerGlyGly/Q192M	Rattus norvegicus	N-succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin	0.0033	0.18	0.019	pH 8, 37 °C	Open record: trypsin variant D189S/TyrGly217-219SerGlyGly/Q192M, N-succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin
trypsinvariant D189S/TyrGly217-219SerGlyGly/Q192M	Rattus norvegicus	N-succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin	0.002	0.23	0.0088	pH 8, 37 °C	Open record: trypsin variant D189S/TyrGly217-219SerGlyGly/Q192M, N-succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin
trypsinvariant D189S/TyrGly217-219SerGlyGly/Q192M	Rattus norvegicus	N-succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin	0.0028	0.18	0.016	pH 8, 37 °C	Open record: trypsin variant D189S/TyrGly217-219SerGlyGly/Q192M, N-succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin
trypsinvariant D189S/TyrGly217-219SerGlyGly/Q192M	Rattus norvegicus	N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin	0.005	0.23	0.0213	pH 8, 37 °C	Open record: trypsin variant D189S/TyrGly217-219SerGlyGly/Q192M, N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
trypsinvariant D189S/TyrGly217-219SerGlyGly/Q192M	Rattus norvegicus	N-succinyl-Ala-Ala-Pro-Trp-7-amido-4-methylcoumarin	0.0027	0.14	0.0192	pH 8, 37 °C	Open record: trypsin variant D189S/TyrGly217-219SerGlyGly/Q192M, N-succinyl-Ala-Ala-Pro-Trp-7-amido-4-methylcoumarin
trypsinvariant D189S/TyrGly217-219SerGlyGly/Q192M	Rattus norvegicus	N-succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin	0.03	0.19	0.1622	pH 8, 37 °C	Open record: trypsin variant D189S/TyrGly217-219SerGlyGly/Q192M, N-succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin
trypsinvariant D189S/ΔD223	Rattus norvegicus	N-succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin	0.0017	0.38	0.0043	pH 8, 37 °C	Open record: trypsin variant D189S/ΔD223, N-succinyl-Ala-Ala-Pro-Arg-7-amido-4-methylcoumarin
trypsinvariant D189S/ΔD223	Rattus norvegicus	N-succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin	0.0022	0.91	0.0023	pH 8, 37 °C	Open record: trypsin variant D189S/ΔD223, N-succinyl-Ala-Ala-Pro-Leu-7-amido-4-methylcoumarin
trypsinvariant D189S/ΔD223	Rattus norvegicus	N-succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin	0.004	1.22	0.0033	pH 8, 37 °C	Open record: trypsin variant D189S/ΔD223, N-succinyl-Ala-Ala-Pro-Lys-7-amido-4-methylcoumarin
trypsinvariant D189S/ΔD223	Rattus norvegicus	N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin	0.004	1.25	0.0032	pH 8, 37 °C	Open record: trypsin variant D189S/ΔD223, N-succinyl-Ala-Ala-Pro-Phe-7-amido-4-methylcoumarin
trypsinvariant D189S/ΔD223	Rattus norvegicus	N-succinyl-Ala-Ala-Pro-Trp-7-amido-4-methylcoumarin	1.7 × 10⁻⁴	0.42	5 × 10⁻⁴	pH 8, 37 °C	Open record: trypsin variant D189S/ΔD223, N-succinyl-Ala-Ala-Pro-Trp-7-amido-4-methylcoumarin
trypsinvariant D189S/ΔD223	Rattus norvegicus	N-succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin	0.08	0.5	0.16	pH 8, 37 °C	Open record: trypsin variant D189S/ΔD223, N-succinyl-Ala-Ala-Pro-Tyr-7-amido-4-methylcoumarin