Values CatLog read from this paper
Acetyltransfer in natural product biosynthesis--functional cloning and molecular analysis of vinorine synthase
PMID 15110860doi:10.1016/j.bmc.2004.02.029Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 10 records (18 values) from this paper: vinorine synthase from Rauvolfia serpentina.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| vinorine synthase | Rauvolfia serpentina | 11-methoxy-16-epi-vellosiminerecombinant (E. coli, pQE2) | 0.065 | 0.0075 | 8.7 calc. | pH 7, 30 °C | Open record: vinorine synthase, 11-methoxy-16-epi-vellosimine |
| vinorine synthase | Rauvolfia serpentina | 11-methoxy-16-epi-vellosiminemean of at least three independent experiments | 0.103 | 0.02 | 5.2 calc. | pH 7, 30 °C | Open record: vinorine synthase, 11-methoxy-16-epi-vellosimine |
| vinorine synthase | Rauvolfia serpentina | acetyl-CoA | — | 0.057 | — | pH 7, 30 °C | Open record: vinorine synthase, acetyl-CoA |
| vinorine synthase | Rauvolfia serpentina | CoA | — | 0.063 | — | pH 7, 30 °C | Open record: vinorine synthase, CoA |
| vinorine synthase | Rauvolfia serpentina | vinorine | 0.735 | 0.01 | 74 calc. | pH 7, 30 °C | Open record: vinorine synthase, vinorine |
| vinorine synthasevariant C149A | Rauvolfia serpentina | 11-methoxy-16-epi-vellosimine | 0.0083 | 0.0011 | 7.6 calc. | pH 7, 30 °C | Open record: vinorine synthase variant C149A, 11-methoxy-16-epi-vellosimine |
| vinorine synthasevariant C89A | Rauvolfia serpentina | 11-methoxy-16-epi-vellosimine | 0.068 | 0.027 | 2.5 calc. | pH 7, 30 °C | Open record: vinorine synthase variant C89A, 11-methoxy-16-epi-vellosimine |
| vinorine synthasevariant N293A | Rauvolfia serpentina | 11-methoxy-16-epi-vellosimine | 0.043 | 0.0095 | 4.6 calc. | pH 7, 30 °C | Open record: vinorine synthase variant N293A, 11-methoxy-16-epi-vellosimine |
| vinorine synthasevariant S29A | Rauvolfia serpentina | 11-methoxy-16-epi-vellosimine | 0.018 | 0.0047 | 3.9 calc. | pH 7, 30 °C | Open record: vinorine synthase variant S29A, 11-methoxy-16-epi-vellosimine |
| vinorine synthasevariant S413A | Rauvolfia serpentina | 11-methoxy-16-epi-vellosimine | 0.085 | 0.021 | 4.0 calc. | pH 7, 30 °C | Open record: vinorine synthase variant S413A, 11-methoxy-16-epi-vellosimine |