Values CatLog read from this paper
Kinetic and mechanistic studies of a cell cycle protein phosphatase Cdc14
PMID 15128740doi:10.1074/jbc.m402217200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 37 records (97 values) from this paper: Cdc14, hCdc14A (human Cdc14A) from Saccharomyces cerevisiae, Homo sapiens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Cdc14 | Saccharomyces cerevisiae | 2-chloro-4-nitrophenyl phosphate | 3.09 | 0.4 | 7.725 | pH 7, 30 °C | Open record: Cdc14, 2-chloro-4-nitrophenyl phosphate |
| Cdc14 | Saccharomyces cerevisiae | 3-O-methylfluorescein phosphate | 3.4 | 1 | 3.4 | pH 7, 30 °C | Open record: Cdc14, 3-O-methylfluorescein phosphate |
| Cdc14 | Saccharomyces cerevisiae | 4-chlorophenyl phosphate | 4.2 | 11.1 | 0.376 | pH 7, 30 °C | Open record: Cdc14, 4-chlorophenyl phosphate |
| Cdc14 | Saccharomyces cerevisiae | 4-cyanophenyl phosphate | 3.35 | 10.8 | 0.311 | pH 7, 30 °C | Open record: Cdc14, 4-cyanophenyl phosphate |
| Cdc14 | Saccharomyces cerevisiae | 4-methylumbelliferyl phosphate | 7 | 2.1 | 3.305 | pH 7, 30 °C | Open record: Cdc14, 4-methylumbelliferyl phosphate |
| Cdc14 | Saccharomyces cerevisiae | 4-trifluoromethylphenyl phosphate | — | — | 0.44 | pH 7, 30 °C | Open record: Cdc14, 4-trifluoromethylphenyl phosphate |
| Cdc14 | Saccharomyces cerevisiae | 5-fluoro-4-methylumbelliferyl phosphate | 6.8 | 1.7 | 3.72 | pH 7, 30 °C | Open record: Cdc14, 5-fluoro-4-methylumbelliferyl phosphate |
| Cdc14 | Saccharomyces cerevisiae | 6,8-difluoro-4-methylumbelliferyl phosphate | 8.1 | 0.0105 | 771 | pH 7, 30 °C | Open record: Cdc14, 6,8-difluoro-4-methylumbelliferyl phosphate |
| Cdc14 | Saccharomyces cerevisiae | 6,8-difluoro-4-methylumbelliferyl phosphate | 10.5 | — | — | 30 °C | Open record: Cdc14, 6,8-difluoro-4-methylumbelliferyl phosphate |
| Cdc14 | Saccharomyces cerevisiae | 6-fluoro-4-methylumbelliferyl phosphate | 7.9 | 1.6 | 4.6 | pH 7, 30 °C | Open record: Cdc14, 6-fluoro-4-methylumbelliferyl phosphate |
| Cdc14 | Saccharomyces cerevisiae | 8-fluoro-4-methylumbelliferyl phosphate | 9 | 0.7 | 15 | pH 7, 30 °C | Open record: Cdc14, 8-fluoro-4-methylumbelliferyl phosphate |
| Cdc14 | Saccharomyces cerevisiae | p-nitrophenyl phosphate | 4.5 | 10.6 | 0.424 | pH 7, 30 °C | Open record: Cdc14, p-nitrophenyl phosphate |
| Cdc14 | Saccharomyces cerevisiae | p-nitrophenyl phosphate | — | — | 23 | 30 °C | Open record: Cdc14, p-nitrophenyl phosphate |
| Cdc14 | Saccharomyces cerevisiae | p-nitrophenyl phosphate | 12.9 | — | — | 30 °C | Open record: Cdc14, p-nitrophenyl phosphate |
| Cdc14 | Saccharomyces cerevisiae | phenyl phosphate | 2.55 | 11.1 | 0.225 | pH 7, 30 °C | Open record: Cdc14, phenyl phosphate |
| Cdc14 | Saccharomyces cerevisiae | phosphorylated human Cdh1 (hCdh1) protein, His6-tagged, from okadaic acid-treated Sf9 cellsfull-length | — | — | 180 | pH 7, 25 °C | Open record: Cdc14, phosphorylated human Cdh1 (hCdh1) protein, His6-tagged, from okadaic acid-treated Sf9 cells |
| Cdc14 | Saccharomyces cerevisiae | phosphorylated human Cdh1 (hCdh1) protein, His6-tagged, from okadaic acid-treated Sf9 cellscatalytic domain residues 1-374 | — | — | 130 | pH 7, 25 °C | Open record: Cdc14, phosphorylated human Cdh1 (hCdh1) protein, His6-tagged, from okadaic acid-treated Sf9 cells |
| Cdc14 | Saccharomyces cerevisiae | β-naphthyl phosphate | 4.2 | 2 | 2 | pH 7, 30 °C | Open record: Cdc14, β-naphthyl phosphate |
| Cdc14variant D129N | Saccharomyces cerevisiae | 6,8-difluoro-4-methylumbelliferyl phosphate | 0.13 | 7.9 × 10⁻⁴ | 160 | pH 7, 30 °C | Open record: Cdc14 variant D129N, 6,8-difluoro-4-methylumbelliferyl phosphate |
| Cdc14variant D129N | Saccharomyces cerevisiae | p-nitrophenyl phosphate | 0.13 | 0.87 | 0.149 | pH 7, 30 °C | Open record: Cdc14 variant D129N, p-nitrophenyl phosphate |
| Cdc14variant D143N | Saccharomyces cerevisiae | 6,8-difluoro-4-methylumbelliferyl phosphate | 5.4 | 0.0061 | 880 | pH 7, 30 °C | Open record: Cdc14 variant D143N, 6,8-difluoro-4-methylumbelliferyl phosphate |
| Cdc14variant D143N | Saccharomyces cerevisiae | p-nitrophenyl phosphate | 3.5 | 9.6 | 0.364 | pH 7, 30 °C | Open record: Cdc14 variant D143N, p-nitrophenyl phosphate |
| Cdc14variant D177N | Saccharomyces cerevisiae | 6,8-difluoro-4-methylumbelliferyl phosphate | 1.1 | 7.1 × 10⁻⁴ | 1.5 × 10³ | pH 7, 30 °C | Open record: Cdc14 variant D177N, 6,8-difluoro-4-methylumbelliferyl phosphate |
| Cdc14variant D177N | Saccharomyces cerevisiae | p-nitrophenyl phosphate | 0.86 | 1 | 0.86 | pH 7, 30 °C | Open record: Cdc14 variant D177N, p-nitrophenyl phosphate |
| Cdc14variant D253A | Saccharomyces cerevisiae | 6,8-difluoro-4-methylumbelliferyl phosphate | 0.007 | 7.9 × 10⁻⁶ | 880 | pH 7, 30 °C | Open record: Cdc14 variant D253A, 6,8-difluoro-4-methylumbelliferyl phosphate |
| Cdc14variant D253A | Saccharomyces cerevisiae | p-nitrophenyl phosphate | 0.007 | 0.11 | 0.06 | pH 7, 30 °C | Open record: Cdc14 variant D253A, p-nitrophenyl phosphate |
| Cdc14variant D253N | Saccharomyces cerevisiae | 6,8-difluoro-4-methylumbelliferyl phosphate | 0.08 | 2.5 × 10⁻⁴ | 320 | pH 7, 30 °C | Open record: Cdc14 variant D253N, 6,8-difluoro-4-methylumbelliferyl phosphate |
| Cdc14variant D253N | Saccharomyces cerevisiae | p-nitrophenyl phosphate | 0.09 | 5.75 | 0.016 | pH 7, 30 °C | Open record: Cdc14 variant D253N, p-nitrophenyl phosphate |
| Cdc14variant D50N | Saccharomyces cerevisiae | p-nitrophenyl phosphate | 0.14 | 0.4 | 0.35 | pH 7, 30 °C | Open record: Cdc14 variant D50N, p-nitrophenyl phosphate |
| Cdc14variant E168Q | Saccharomyces cerevisiae | 6,8-difluoro-4-methylumbelliferyl phosphate | 8.7 | 0.0166 | 520 | pH 7, 30 °C | Open record: Cdc14 variant E168Q, 6,8-difluoro-4-methylumbelliferyl phosphate |
| Cdc14variant E168Q | Saccharomyces cerevisiae | p-nitrophenyl phosphate | 4.7 | 12.9 | 0.364 | pH 7, 30 °C | Open record: Cdc14 variant E168Q, p-nitrophenyl phosphate |
| Cdc14variant E171Q | Saccharomyces cerevisiae | p-nitrophenyl phosphate | 0.94 | 2.8 | 0.336 | pH 7, 30 °C | Open record: Cdc14 variant E171Q, p-nitrophenyl phosphate |
| Cdc14variant E174Q | Saccharomyces cerevisiae | 6,8-difluoro-4-methylumbelliferyl phosphate | 8.2 | 0.0096 | 850 | pH 7, 30 °C | Open record: Cdc14 variant E174Q, 6,8-difluoro-4-methylumbelliferyl phosphate |
| Cdc14variant E174Q | Saccharomyces cerevisiae | p-nitrophenyl phosphate | 4.3 | 11.2 | 0.383 | pH 7, 30 °C | Open record: Cdc14 variant E174Q, p-nitrophenyl phosphate |
| Cdc14variant E308Q | Saccharomyces cerevisiae | 6,8-difluoro-4-methylumbelliferyl phosphate | 5.7 | 0.0087 | 650 | pH 7, 30 °C | Open record: Cdc14 variant E308Q, 6,8-difluoro-4-methylumbelliferyl phosphate |
| Cdc14variant E308Q | Saccharomyces cerevisiae | p-nitrophenyl phosphate | 4 | 11.3 | 0.353 | pH 7, 30 °C | Open record: Cdc14 variant E308Q, p-nitrophenyl phosphate |
| hCdc14A (human Cdc14A) | Homo sapiens | phosphorylated human Cdh1 (hCdh1) protein, His6-tagged, from okadaic acid-treated Sf9 cells | — | — | 400 | pH 7, 25 °C | Open record: hCdc14A (human Cdc14A), phosphorylated human Cdh1 (hCdh1) protein, His6-tagged, from okadaic acid-treated Sf9 cells |