CatLogenzyme kinetics catalog

Values CatLog read from this paper

Mechanism for the hydrolysis of organophosphates by the bacterial phosphotriesterase

PMID 15134445doi:10.1021/bi0497805Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 25 records (75 values) from this paper: phosphotriesterase (PTE) from Pseudomonas diminuta.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 3.1.8.1

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
phosphotriesterase (PTE)Pseudomonas diminutadiethyl 4-chlorophenyl phosphateZn/Zn PTE (apo-enzyme reconstituted with 2 equiv ZnCl2)0.361.60.22pH 9, 30 °COpen
phosphotriesterase (PTE)Pseudomonas diminutadiethyl 4-chlorophenyl phosphateCd/Cd PTE (apo-enzyme reconstituted with 2 equiv CdCl2)0.2370.033pH 9, 30 °COpen
phosphotriesterase (PTE)Pseudomonas diminutadiethyl 4-chlorophenyl phosphate0.133.30.039pH 9, 30 °COpen
phosphotriesterase (PTE)Pseudomonas diminutadiethyl 4-nitrophenyl phosphateZn/Zn PTE (apo-enzyme reconstituted with 2 equiv ZnCl2)2.3 × 10³0.346.8 × 10³pH 9, 30 °COpen
phosphotriesterase (PTE)Pseudomonas diminutadiethyl 4-nitrophenyl phosphateCd/Cd PTE (apo-enzyme reconstituted with 2 equiv CdCl2)2.5 × 10³1.91.32 × 10³pH 9, 30 °COpen
phosphotriesterase (PTE)Pseudomonas diminutadiethyl 4-nitrophenyl phosphate2.06 × 10³0.742.8 × 10³pH 9, 30 °COpen
phosphotriesterase (PTE)Pseudomonas diminutaO,O-diethyl O-(4-chlorophenyl) phosphorothioateZn/Zn PTE (apo-enzyme reconstituted with 2 equiv ZnCl2)2.20.54.4pH 9, 30 °COpen
phosphotriesterase (PTE)Pseudomonas diminutaO,O-diethyl O-(4-chlorophenyl) phosphorothioateCd/Cd PTE (apo-enzyme reconstituted with 2 equiv CdCl2)1.10.34pH 9, 30 °COpen
phosphotriesterase (PTE)Pseudomonas diminutaO,O-diethyl O-(4-chlorophenyl) phosphorothioate2.40.64.1pH 9, 30 °COpen
phosphotriesterase (PTE)Pseudomonas diminutaO,O-diethyl O-(4-nitrophenyl) phosphorothioateZn/Zn PTE (apo-enzyme reconstituted with 2 equiv ZnCl2)7201.5480pH 9, 30 °COpen
phosphotriesterase (PTE)Pseudomonas diminutaO,O-diethyl O-(4-nitrophenyl) phosphorothioateCd/Cd PTE (apo-enzyme reconstituted with 2 equiv CdCl2)6001.7350pH 9, 30 °COpen
phosphotriesterase (PTE)Pseudomonas diminutaO,O-diethyl O-(4-nitrophenyl) phosphorothioate3000.6500pH 9, 30 °COpen
phosphotriesterase (PTE)variant D233APseudomonas diminutadiethyl 4-chlorophenyl phosphate4.43.41.31pH 9, 30 °COpen
phosphotriesterase (PTE)variant D233APseudomonas diminutadiethyl 4-nitrophenyl phosphate2800.57490pH 9, 30 °COpen
phosphotriesterase (PTE)variant D233NPseudomonas diminutadiethyl 4-chlorophenyl phosphate0.882.20.4pH 9, 30 °COpen
phosphotriesterase (PTE)variant D233NPseudomonas diminutadiethyl 4-nitrophenyl phosphate6800.87780pH 9, 30 °COpen
phosphotriesterase (PTE)variant D301APseudomonas diminutadiethyl 4-chlorophenyl phosphate0.0150.260.06pH 9, 30 °COpen
phosphotriesterase (PTE)variant D301APseudomonas diminutadiethyl 4-nitrophenyl phosphate11.20.3433pH 9, 30 °COpen
phosphotriesterase (PTE)variant D301NPseudomonas diminutadiethyl 4-nitrophenyl phosphate0.550.22.8pH 9, 30 °COpen
phosphotriesterase (PTE)variant H254APseudomonas diminutadiethyl 4-chlorophenyl phosphate1291.4pH 9, 30 °COpen
phosphotriesterase (PTE)variant H254APseudomonas diminutadiethyl 4-nitrophenyl phosphate2200.141.6 × 10³pH 9, 30 °COpen
phosphotriesterase (PTE)variant H254NPseudomonas diminutadiethyl 4-chlorophenyl phosphate0.560.870.64pH 9, 30 °COpen
phosphotriesterase (PTE)variant H254NPseudomonas diminutadiethyl 4-nitrophenyl phosphate17.80.021860pH 9, 30 °COpen
phosphotriesterase (PTE)variant Y309FPseudomonas diminutadiethyl 4-chlorophenyl phosphate0.2430.079pH 9, 30 °COpen
phosphotriesterase (PTE)variant Y309FPseudomonas diminutadiethyl 4-nitrophenyl phosphate1.7 × 10³0.82.3 × 10³pH 9, 30 °COpen