Values CatLog read from this paper
Identifying determinants of NADPH specificity in Baeyer-Villiger monooxygenases
PMID 15153101doi:10.1111/j.1432-1033.2004.04126.xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 16 records (46 values) from this paper: 4-hydroxyacetophenone monooxygenase, cyclohexanone monooxygenase from Pseudomonas fluorescens, Acinetobacter sp..
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 4-hydroxyacetophenone monooxygenase | Pseudomonas fluorescens | NADH | 1.7 | 1.44 | 1.2 | pH 7.5, 25 °C | Open record: 4-hydroxyacetophenone monooxygenase, NADH |
| 4-hydroxyacetophenone monooxygenase | Pseudomonas fluorescens | NADPH | 10.1 | 0.012 | 840 | pH 7.5, 25 °C | Open record: 4-hydroxyacetophenone monooxygenase, NADPH |
| 4-hydroxyacetophenone monooxygenasevariant K439A | Pseudomonas fluorescens | NADH | 2.4 | 0.52 | 4.6 | pH 7.5, 25 °C | Open record: 4-hydroxyacetophenone monooxygenase variant K439A, NADH |
| 4-hydroxyacetophenone monooxygenasevariant K439A | Pseudomonas fluorescens | NADPH | 1.6 | 0.2 | 8 | pH 7.5, 25 °C | Open record: 4-hydroxyacetophenone monooxygenase variant K439A, NADPH |
| 4-hydroxyacetophenone monooxygenasevariant K439F | Pseudomonas fluorescens | NADH | 2.4 | 0.3 | 8 | pH 7.5, 25 °C | Open record: 4-hydroxyacetophenone monooxygenase variant K439F, NADH |
| 4-hydroxyacetophenone monooxygenasevariant K439F | Pseudomonas fluorescens | NADPH | 1 | 0.08 | 13 | pH 7.5, 25 °C | Open record: 4-hydroxyacetophenone monooxygenase variant K439F, NADPH |
| 4-hydroxyacetophenone monooxygenasevariant K439N | Pseudomonas fluorescens | NADH | 5.1 | 0.78 | 7 | pH 7.5, 25 °C | Open record: 4-hydroxyacetophenone monooxygenase variant K439N, NADH |
| 4-hydroxyacetophenone monooxygenasevariant K439N | Pseudomonas fluorescens | NADPH | 3.5 | 0.078 | 45 | pH 7.5, 25 °C | Open record: 4-hydroxyacetophenone monooxygenase variant K439N, NADPH |
| 4-hydroxyacetophenone monooxygenasevariant K439P | Pseudomonas fluorescens | NADH | 0.7 | 1.28 | 0.5 | pH 7.5, 25 °C | Open record: 4-hydroxyacetophenone monooxygenase variant K439P, NADH |
| 4-hydroxyacetophenone monooxygenasevariant K439P | Pseudomonas fluorescens | NADPH | 0.2 | 0.017 | 12 | pH 7.5, 25 °C | Open record: 4-hydroxyacetophenone monooxygenase variant K439P, NADPH |
| 4-hydroxyacetophenone monooxygenasevariant R339A | Pseudomonas fluorescens | NADH | 0.19 | 3 | 0.063 | pH 7.5, 25 °C | Open record: 4-hydroxyacetophenone monooxygenase variant R339A, NADH |
| 4-hydroxyacetophenone monooxygenasevariant R339A | Pseudomonas fluorescens | NADPH | — | — | 3 × 10⁻⁴ | pH 7.5, 25 °C | Open record: 4-hydroxyacetophenone monooxygenase variant R339A, NADPH |
| cyclohexanone monooxygenase | Acinetobacter sp. | NADH | 1.4 | 0.42 | 3.3 | pH 9 | Open record: cyclohexanone monooxygenase, NADH |
| cyclohexanone monooxygenase | Acinetobacter sp. | NADPH | 14 | 0.0073 | 1.9 × 10³ | pH 9 | Open record: cyclohexanone monooxygenase, NADPH |
| cyclohexanone monooxygenasevariant K326A | Acinetobacter sp. | NADH | 0.5 | 0.23 | 2.2 | pH 9 | Open record: cyclohexanone monooxygenase variant K326A, NADH |
| cyclohexanone monooxygenasevariant K326A | Acinetobacter sp. | NADPH | 3.7 | 0.16 | 23 | pH 9 | Open record: cyclohexanone monooxygenase variant K326A, NADPH |