Values CatLog read from this paper
Evolution of enzymatic activities in the orotidine 5'-monophosphate decarboxylase suprafamily: mechanistic evidence for a proton relay system in the active site of 3-keto-L-gulonate 6-phosphate…
PMID 15157077doi:10.1021/bi049741tOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 23 records (69 values) from this paper: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 51 | 0.67 | 77 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD), 3-keto-L-gulonate 6-phosphate |
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD)variant D67A | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 2.4 | 0.5 | 4.8 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) variant D67A, 3-keto-L-gulonate 6-phosphate |
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD)variant D67A/H136A | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 0.19 | 0.27 | 0.7 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) variant D67A/H136A, 3-keto-L-gulonate 6-phosphate |
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD)variant D67N | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 1.1 | 0.41 | 2.7 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) variant D67N, 3-keto-L-gulonate 6-phosphate |
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD)variant D67N/H136A | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 1.3 | 0.3 | 4.3 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) variant D67N/H136A, 3-keto-L-gulonate 6-phosphate |
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD)variant E112A | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 0.3 | 0.34 | 0.88 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) variant E112A, 3-keto-L-gulonate 6-phosphate |
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD)variant E112A/H136A | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 0.45 | 0.65 | 0.69 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) variant E112A/H136A, 3-keto-L-gulonate 6-phosphate |
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD)variant E112A/H136A/R139V | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 0.21 | 0.77 | 0.27 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) variant E112A/H136A/R139V, 3-keto-L-gulonate 6-phosphate |
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD)variant E112Q | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 4 | 0.31 | 13 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) variant E112Q, 3-keto-L-gulonate 6-phosphate |
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD)variant E112Q/H136A | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 0.23 | 0.15 | 1.5 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) variant E112Q/H136A, 3-keto-L-gulonate 6-phosphate |
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD)variant E112Q/H136A/R139V | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 0.2 | 0.22 | 0.91 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) variant E112Q/H136A/R139V, 3-keto-L-gulonate 6-phosphate |
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD)variant H136A | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 2.4 | 0.7 | 3.4 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) variant H136A, 3-keto-L-gulonate 6-phosphate |
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD)variant H136A/R139V | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 9.2 | 1.4 | 6.6 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) variant H136A/R139V, 3-keto-L-gulonate 6-phosphate |
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD)variant H136N | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 4.3 | 0.37 | 13 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) variant H136N, 3-keto-L-gulonate 6-phosphate |
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD)variant H136Q | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 1.4 | 0.52 | 2.7 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) variant H136Q, 3-keto-L-gulonate 6-phosphate |
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD)variant K64A | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 8.3 | 0.51 | 16 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) variant K64A, 3-keto-L-gulonate 6-phosphate |
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD)variant K64A/E112A/H136A/R139V | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 0.26 | 1.4 | 0.19 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) variant K64A/E112A/H136A/R139V, 3-keto-L-gulonate 6-phosphate |
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD)variant K64A/E112Q/H136A | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 2.9 | 0.55 | 5.3 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) variant K64A/E112Q/H136A, 3-keto-L-gulonate 6-phosphate |
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD)variant K64A/H136A | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 0.75 | 0.58 | 1.3 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) variant K64A/H136A, 3-keto-L-gulonate 6-phosphate |
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD)variant K64A/R139V | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 1.2 | 0.9 | 1.3 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) variant K64A/R139V, 3-keto-L-gulonate 6-phosphate |
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD)variant K64R | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 17 | 0.3 | 57 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) variant K64R, 3-keto-L-gulonate 6-phosphate |
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD)variant K64R/H136A | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 4.2 | 0.44 | 9.5 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) variant K64R/H136A, 3-keto-L-gulonate 6-phosphate |
| 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD)variant R139V | Escherichia coli | 3-keto-L-gulonate 6-phosphate | 8.9 | 0.36 | 25 | pH 7.5, 25 °C | Open record: 3-keto-L-gulonate 6-phosphate decarboxylase (KGPDC, UlaD) variant R139V, 3-keto-L-gulonate 6-phosphate |