Values CatLog read from this paper
Probing the specificity of the subclass B3 FEZ-1 metallo-beta-lactamase by site-directed mutagenesis
PMID 15159411doi:10.1074/jbc.m403671200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 70 records (186 values) from this paper: FEZ-1 metallo-β-lactamase from Fluoribacter gormanii.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| FEZ-1 metallo-β-lactamase | Fluoribacter gormanii | 7-aminocephalosporanic acid | 30 | 1 | 30 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase, 7-aminocephalosporanic acid |
| FEZ-1 metallo-β-lactamase | Fluoribacter gormanii | Benzylpenicillin[Zn2+] <= 0.4 µM (low zinc) | 70 | 0.59 | 110 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase, Benzylpenicillin |
| FEZ-1 metallo-β-lactamase | Fluoribacter gormanii | Benzylpenicillin[Zn2+] = 100 µM added zinc | 50 | 0.28 | 180 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase, Benzylpenicillin |
| FEZ-1 metallo-β-lactamase | Fluoribacter gormanii | Biapenem | — | — | 70 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase, Biapenem |
| FEZ-1 metallo-β-lactamase | Fluoribacter gormanii | Cefotaxime[Zn2+] <= 0.4 µM (low zinc) | 170 | 0.07 | 2.4 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase, Cefotaxime |
| FEZ-1 metallo-β-lactamase | Fluoribacter gormanii | Cefotaxime[Zn2+] = 100 µM added zinc | 430 | 0.07 | 6.1 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase, Cefotaxime |
| FEZ-1 metallo-β-lactamase | Fluoribacter gormanii | Cefuroxime[Zn2+] <= 0.4 µM (low zinc) | 320 | 0.05 | 6.6 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase, Cefuroxime |
| FEZ-1 metallo-β-lactamase | Fluoribacter gormanii | Cefuroxime[Zn2+] = 100 µM added zinc | 330 | 0.035 | 9.4 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase, Cefuroxime |
| FEZ-1 metallo-β-lactamase | Fluoribacter gormanii | Cephalothin | 300 | 0.12 | 2.5 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase, Cephalothin |
| FEZ-1 metallo-β-lactamase | Fluoribacter gormanii | Imipenem[Zn2+] <= 0.4 µM (low zinc) | — | — | 200 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase, Imipenem |
| FEZ-1 metallo-β-lactamase | Fluoribacter gormanii | Imipenem[Zn2+] = 100 µM added zinc | — | — | 2 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase, Imipenem |
| FEZ-1 metallo-β-lactamase | Fluoribacter gormanii | Meropenem | 45 | 0.085 | 500 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase, Meropenem |
| FEZ-1 metallo-β-lactamase | Fluoribacter gormanii | Nitrocefin[Zn2+] <= 0.4 µM (low zinc) | 100 | 0.09 | 900 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase, Nitrocefin |
| FEZ-1 metallo-β-lactamase | Fluoribacter gormanii | Nitrocefin[Zn2+] = 100 µM added zinc | 600 | 0.19 | 3.2 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase, Nitrocefin |
| FEZ-1 metallo-β-lactamasevariant H121A | Fluoribacter gormanii | Benzylpenicillin[Zn2+] <= 0.4 µM (low zinc) | 15 | 0.5 | 30 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant H121A, Benzylpenicillin |
| FEZ-1 metallo-β-lactamasevariant H121A | Fluoribacter gormanii | Benzylpenicillin[Zn2+] = 100 µM added zinc | 600 | 2.2 | 270 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant H121A, Benzylpenicillin |
| FEZ-1 metallo-β-lactamasevariant H121A | Fluoribacter gormanii | Cefotaxime[Zn2+] <= 0.4 µM (low zinc) | 60 | 0.1 | 600 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant H121A, Cefotaxime |
| FEZ-1 metallo-β-lactamasevariant H121A | Fluoribacter gormanii | Cefotaxime[Zn2+] = 100 µM added zinc | 120 | 0.06 | 2 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant H121A, Cefotaxime |
| FEZ-1 metallo-β-lactamasevariant H121A | Fluoribacter gormanii | Cefuroxime[Zn2+] <= 0.4 µM (low zinc) | 27 | 0.055 | 500 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant H121A, Cefuroxime |
| FEZ-1 metallo-β-lactamasevariant H121A | Fluoribacter gormanii | Cefuroxime[Zn2+] = 100 µM added zinc | 110 | 0.055 | 2.1 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant H121A, Cefuroxime |
| FEZ-1 metallo-β-lactamasevariant H121A | Fluoribacter gormanii | Imipenem[Zn2+] <= 0.4 µM (low zinc) | — | — | 7 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant H121A, Imipenem |
| FEZ-1 metallo-β-lactamasevariant H121A | Fluoribacter gormanii | Imipenem[Zn2+] = 100 µM added zinc | — | — | 70 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant H121A, Imipenem |
| FEZ-1 metallo-β-lactamasevariant H121A | Fluoribacter gormanii | Nitrocefin[Zn2+] <= 0.4 µM (low zinc) | 7 | 0.1 | 70 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant H121A, Nitrocefin |
| FEZ-1 metallo-β-lactamasevariant H121A | Fluoribacter gormanii | Nitrocefin[Zn2+] = 100 µM added zinc | 220 | 0.13 | 1.7 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant H121A, Nitrocefin |
| FEZ-1 metallo-β-lactamasevariant N225A | Fluoribacter gormanii | Benzylpenicillin[Zn2+] <= 0.4 µM (low zinc) | 25 | 0.6 | 40 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant N225A, Benzylpenicillin |
| FEZ-1 metallo-β-lactamasevariant N225A | Fluoribacter gormanii | Benzylpenicillin[Zn2+] = 100 µM added zinc | 20 | 0.8 | 30 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant N225A, Benzylpenicillin |
| FEZ-1 metallo-β-lactamasevariant N225A | Fluoribacter gormanii | Biapenem | — | — | 4 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant N225A, Biapenem |
| FEZ-1 metallo-β-lactamasevariant N225A | Fluoribacter gormanii | Cefotaxime[Zn2+] <= 0.4 µM (low zinc) | 800 | 0.12 | 7 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant N225A, Cefotaxime |
| FEZ-1 metallo-β-lactamasevariant N225A | Fluoribacter gormanii | Cefotaxime[Zn2+] = 100 µM added zinc | 1.8 × 10³ | 0.12 | 1.5 × 10⁴ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant N225A, Cefotaxime |
| FEZ-1 metallo-β-lactamasevariant N225A | Fluoribacter gormanii | Cefuroxime[Zn2+] <= 0.4 µM (low zinc) | 350 | 0.05 | 8 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant N225A, Cefuroxime |
| FEZ-1 metallo-β-lactamasevariant N225A | Fluoribacter gormanii | Cefuroxime[Zn2+] = 100 µM added zinc | 600 | 0.03 | 1.9 × 10⁴ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant N225A, Cefuroxime |
| FEZ-1 metallo-β-lactamasevariant N225A | Fluoribacter gormanii | Imipenem[Zn2+] <= 0.4 µM (low zinc) | 11 | 1.25 | 10 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant N225A, Imipenem |
| FEZ-1 metallo-β-lactamasevariant N225A | Fluoribacter gormanii | Imipenem[Zn2+] = 100 µM added zinc | 5.5 | 0.8 | 7 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant N225A, Imipenem |
| FEZ-1 metallo-β-lactamasevariant N225A | Fluoribacter gormanii | Meropenem | 35 | 0.8 | 40 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant N225A, Meropenem |
| FEZ-1 metallo-β-lactamasevariant N225A | Fluoribacter gormanii | Nitrocefin[Zn2+] <= 0.4 µM (low zinc) | 16 | 0.02 | 800 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant N225A, Nitrocefin |
| FEZ-1 metallo-β-lactamasevariant N225A | Fluoribacter gormanii | Nitrocefin[Zn2+] = 100 µM added zinc | 60 | 0.18 | 3 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant N225A, Nitrocefin |
| FEZ-1 metallo-β-lactamasevariant S221A | Fluoribacter gormanii | Benzylpenicillin[Zn2+] <= 0.4 µM (low zinc) | 12 | 0.96 | 13 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant S221A, Benzylpenicillin |
| FEZ-1 metallo-β-lactamasevariant S221A | Fluoribacter gormanii | Benzylpenicillin[Zn2+] = 100 µM added zinc | 10 | 0.7 | 13 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant S221A, Benzylpenicillin |
| FEZ-1 metallo-β-lactamasevariant S221A | Fluoribacter gormanii | Biapenem | — | — | 5 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant S221A, Biapenem |
| FEZ-1 metallo-β-lactamasevariant S221A | Fluoribacter gormanii | Cefotaxime[Zn2+] <= 0.4 µM (low zinc) | 90 | 0.085 | 1.1 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant S221A, Cefotaxime |
| FEZ-1 metallo-β-lactamasevariant S221A | Fluoribacter gormanii | Cefotaxime[Zn2+] = 100 µM added zinc | 480 | 0.44 | 1.1 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant S221A, Cefotaxime |
| FEZ-1 metallo-β-lactamasevariant S221A | Fluoribacter gormanii | Cefuroxime[Zn2+] <= 0.4 µM (low zinc) | 160 | 0.085 | 1.9 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant S221A, Cefuroxime |
| FEZ-1 metallo-β-lactamasevariant S221A | Fluoribacter gormanii | Cefuroxime[Zn2+] = 100 µM added zinc | 480 | 0.36 | 1.3 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant S221A, Cefuroxime |
| FEZ-1 metallo-β-lactamasevariant S221A | Fluoribacter gormanii | Imipenem[Zn2+] <= 0.4 µM (low zinc) | — | — | 2 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant S221A, Imipenem |
| FEZ-1 metallo-β-lactamasevariant S221A | Fluoribacter gormanii | Imipenem[Zn2+] = 100 µM added zinc | — | — | 2 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant S221A, Imipenem |
| FEZ-1 metallo-β-lactamasevariant S221A | Fluoribacter gormanii | Meropenem | 0.3 | 0.115 | 3 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant S221A, Meropenem |
| FEZ-1 metallo-β-lactamasevariant S221A | Fluoribacter gormanii | Nitrocefin[Zn2+] <= 0.4 µM (low zinc) | 8 | 0.08 | 100 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant S221A, Nitrocefin |
| FEZ-1 metallo-β-lactamasevariant S221A | Fluoribacter gormanii | Nitrocefin[Zn2+] = 100 µM added zinc | 85 | 0.24 | 350 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant S221A, Nitrocefin |
| FEZ-1 metallo-β-lactamasevariant Y156A | Fluoribacter gormanii | Benzylpenicillin[Zn2+] <= 0.4 µM (low zinc) | 25 | 0.95 | 26 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant Y156A, Benzylpenicillin |
| FEZ-1 metallo-β-lactamasevariant Y156A | Fluoribacter gormanii | Benzylpenicillin[Zn2+] = 100 µM added zinc | 125 | 0.93 | 130 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant Y156A, Benzylpenicillin |
| FEZ-1 metallo-β-lactamasevariant Y156A | Fluoribacter gormanii | Cefotaxime[Zn2+] <= 0.4 µM (low zinc) | 660 | 0.15 | 4.4 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant Y156A, Cefotaxime |
| FEZ-1 metallo-β-lactamasevariant Y156A | Fluoribacter gormanii | Cefotaxime[Zn2+] = 100 µM added zinc | 750 | 0.15 | 5 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant Y156A, Cefotaxime |
| FEZ-1 metallo-β-lactamasevariant Y156A | Fluoribacter gormanii | Cefuroxime[Zn2+] <= 0.4 µM (low zinc) | 550 | 0.06 | 9.2 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant Y156A, Cefuroxime |
| FEZ-1 metallo-β-lactamasevariant Y156A | Fluoribacter gormanii | Cefuroxime[Zn2+] = 100 µM added zinc | 820 | 0.125 | 6.5 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant Y156A, Cefuroxime |
| FEZ-1 metallo-β-lactamasevariant Y156A | Fluoribacter gormanii | Imipenem[Zn2+] <= 0.4 µM (low zinc) | — | — | 20 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant Y156A, Imipenem |
| FEZ-1 metallo-β-lactamasevariant Y156A | Fluoribacter gormanii | Imipenem[Zn2+] = 100 µM added zinc | — | — | 13 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant Y156A, Imipenem |
| FEZ-1 metallo-β-lactamasevariant Y156A | Fluoribacter gormanii | Nitrocefin[Zn2+] <= 0.4 µM (low zinc) | 190 | 0.13 | 1.5 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant Y156A, Nitrocefin |
| FEZ-1 metallo-β-lactamasevariant Y156A | Fluoribacter gormanii | Nitrocefin[Zn2+] = 100 µM added zinc | 50 | 0.02 | 2.4 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant Y156A, Nitrocefin |
| FEZ-1 metallo-β-lactamasevariant Y228A | Fluoribacter gormanii | 7-aminocephalosporanic acid | 20 | 0.88 | 2.4 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant Y228A, 7-aminocephalosporanic acid |
| FEZ-1 metallo-β-lactamasevariant Y228A | Fluoribacter gormanii | Benzylpenicillin[Zn2+] <= 0.4 µM (low zinc) | 125 | 3.6 | 35 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant Y228A, Benzylpenicillin |
| FEZ-1 metallo-β-lactamasevariant Y228A | Fluoribacter gormanii | Benzylpenicillin[Zn2+] = 100 µM added zinc | 40 | 1.3 | 30 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant Y228A, Benzylpenicillin |
| FEZ-1 metallo-β-lactamasevariant Y228A | Fluoribacter gormanii | Cefotaxime[Zn2+] <= 0.4 µM (low zinc) | 1.25 × 10³ | 0.1 | 1.28 × 10⁴ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant Y228A, Cefotaxime |
| FEZ-1 metallo-β-lactamasevariant Y228A | Fluoribacter gormanii | Cefotaxime[Zn2+] = 100 µM added zinc | 7.6 × 10³ | 0.66 | 1.1 × 10⁴ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant Y228A, Cefotaxime |
| FEZ-1 metallo-β-lactamasevariant Y228A | Fluoribacter gormanii | Cefuroxime[Zn2+] <= 0.4 µM (low zinc) | 5.9 × 10³ | 0.16 | 3.5 × 10⁴ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant Y228A, Cefuroxime |
| FEZ-1 metallo-β-lactamasevariant Y228A | Fluoribacter gormanii | Cefuroxime[Zn2+] = 100 µM added zinc | 6.6 × 10³ | 0.13 | 5 × 10⁴ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant Y228A, Cefuroxime |
| FEZ-1 metallo-β-lactamasevariant Y228A | Fluoribacter gormanii | Cephalothin | 2.35 × 10³ | 0.07 | 3.2 × 10⁴ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant Y228A, Cephalothin |
| FEZ-1 metallo-β-lactamasevariant Y228A | Fluoribacter gormanii | Imipenem[Zn2+] <= 0.4 µM (low zinc) | — | — | 20 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant Y228A, Imipenem |
| FEZ-1 metallo-β-lactamasevariant Y228A | Fluoribacter gormanii | Imipenem[Zn2+] = 100 µM added zinc | 14 | 1 | 14 | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant Y228A, Imipenem |
| FEZ-1 metallo-β-lactamasevariant Y228A | Fluoribacter gormanii | Nitrocefin[Zn2+] <= 0.4 µM (low zinc) | 200 | 0.14 | 1.4 × 10³ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant Y228A, Nitrocefin |
| FEZ-1 metallo-β-lactamasevariant Y228A | Fluoribacter gormanii | Nitrocefin[Zn2+] = 100 µM added zinc | 3.25 × 10³ | 0.065 | 5 × 10⁴ | pH 6, 30 °C | Open record: FEZ-1 metallo-β-lactamase variant Y228A, Nitrocefin |