Values CatLog read from this paper
Coenzyme site-directed mutants of photosynthetic A4-GAPDH show selectively reduced NADPH-dependent catalysis, similar to regulatory AB-GAPDH inhibited by oxidized thioredoxin
PMID 15236965doi:10.1016/j.jmb.2004.06.005Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 20 records (40 values) from this paper: glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH), glyceraldehyde-3-phosphate dehydrogenase, photosynthetic AB isoform (native AB-GAPDH) from Spinacia oleracea.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH) | Spinacia oleracea | 1,3-bisphospho-D-glyceratecosubstrate NADPH | — | 0.015 | — | Open record: glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH), 1,3-bisphospho-D-glycerate | |
| glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH) | Spinacia oleracea | 1,3-bisphospho-D-glyceratecosubstrate NADH | — | 0.012 | — | Open record: glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH), 1,3-bisphospho-D-glycerate | |
| glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH) | Spinacia oleracea | NADH | 41 | 0.14 | 300 | Open record: glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH), NADH | |
| glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH) | Spinacia oleracea | NADPH | 61 | 0.029 | 1.8 × 10³ | Open record: glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH), NADPH | |
| glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH)variant S188A | Spinacia oleracea | 1,3-bisphospho-D-glyceratecosubstrate NADPH | — | 0.013 | — | Open record: glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH) variant S188A, 1,3-bisphospho-D-glycerate | |
| glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH)variant S188A | Spinacia oleracea | 1,3-bisphospho-D-glyceratecosubstrate NADH | — | 0.012 | — | Open record: glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH) variant S188A, 1,3-bisphospho-D-glycerate | |
| glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH)variant S188A | Spinacia oleracea | NADH | 32 | 0.168 | 190 | Open record: glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH) variant S188A, NADH | |
| glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH)variant S188A | Spinacia oleracea | NADPH | 32 | 0.05 | 730 | Open record: glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH) variant S188A, NADPH | |
| glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH)variant T33A | Spinacia oleracea | 1,3-bisphospho-D-glyceratecosubstrate NADPH | — | 0.018 | — | Open record: glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH) variant T33A, 1,3-bisphospho-D-glycerate | |
| glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH)variant T33A | Spinacia oleracea | 1,3-bisphospho-D-glyceratecosubstrate NADH | — | 0.012 | — | Open record: glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH) variant T33A, 1,3-bisphospho-D-glycerate | |
| glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH)variant T33A | Spinacia oleracea | NADH | 37 | 0.174 | 220 | Open record: glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH) variant T33A, NADH | |
| glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH)variant T33A | Spinacia oleracea | NADPH | 52 | 0.044 | 1.2 × 10³ | Open record: glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH) variant T33A, NADPH | |
| glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH)variant T33A/S188A | Spinacia oleracea | 1,3-bisphospho-D-glyceratecosubstrate NADPH | — | 0.012 | — | Open record: glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH) variant T33A/S188A, 1,3-bisphospho-D-glycerate | |
| glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH)variant T33A/S188A | Spinacia oleracea | 1,3-bisphospho-D-glyceratecosubstrate NADH | — | 0.01 | — | Open record: glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH) variant T33A/S188A, 1,3-bisphospho-D-glycerate | |
| glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH)variant T33A/S188A | Spinacia oleracea | NADH | 48 | 0.184 | 260 | Open record: glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH) variant T33A/S188A, NADH | |
| glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH)variant T33A/S188A | Spinacia oleracea | NADPH | 42 | 0.05 | 920 | Open record: glyceraldehyde-3-phosphate dehydrogenase, photosynthetic A4 isoform (recombinant GapA homotetramer, A4-GAPDH) variant T33A/S188A, NADPH | |
| glyceraldehyde-3-phosphate dehydrogenase, photosynthetic AB isoform (native AB-GAPDH) | Spinacia oleracea | 1,3-bisphospho-D-glyceratereduced dithiothreitol (reduced AB-GAPDH) | — | 0.02 | — | Open record: glyceraldehyde-3-phosphate dehydrogenase, photosynthetic AB isoform (native AB-GAPDH), 1,3-bisphospho-D-glycerate | |
| glyceraldehyde-3-phosphate dehydrogenase, photosynthetic AB isoform (native AB-GAPDH) | Spinacia oleracea | 1,3-bisphospho-D-glycerateoxidized dithiothreitol (oxidized AB-GAPDH) | — | 0.024 | — | Open record: glyceraldehyde-3-phosphate dehydrogenase, photosynthetic AB isoform (native AB-GAPDH), 1,3-bisphospho-D-glycerate | |
| glyceraldehyde-3-phosphate dehydrogenase, photosynthetic AB isoform (native AB-GAPDH) | Spinacia oleracea | NADPHreduced dithiothreitol (reduced AB-GAPDH) | 59 | 0.05 | 1.2 × 10³ | Open record: glyceraldehyde-3-phosphate dehydrogenase, photosynthetic AB isoform (native AB-GAPDH), NADPH | |
| glyceraldehyde-3-phosphate dehydrogenase, photosynthetic AB isoform (native AB-GAPDH) | Spinacia oleracea | NADPHoxidized dithiothreitol (oxidized AB-GAPDH) | 27 | 0.05 | 550 | Open record: glyceraldehyde-3-phosphate dehydrogenase, photosynthetic AB isoform (native AB-GAPDH), NADPH |