Values CatLog read from this paper
Probing the dynamics of a mobile loop above the active site of L1, a metallo-beta-lactamase from Stenotrophomonas maltophilia, via site-directed mutagenesis and stopped-flow fluorescence…
PMID 15271998doi:10.1074/jbc.m406826200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 24 records (48 values) from this paper: metallo-beta-lactamase L1 from Stenotrophomonas maltophilia.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 24 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| metallo-beta-lactamase L1variant W204F | Stenotrophomonas maltophilia | Cephalothin | 46 | 0.0054 | 8.5 × 10³ calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W204F, Cephalothin |
| metallo-beta-lactamase L1variant W204F | Stenotrophomonas maltophilia | Meropenem | 66 | 0.01 | 6.6 × 10³ calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W204F, Meropenem |
| metallo-beta-lactamase L1variant W204F | Stenotrophomonas maltophilia | Nitrocefin | 30 | 0.0033 | 9.1 × 10³ calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W204F, Nitrocefin |
| metallo-beta-lactamase L1variant W204F | Stenotrophomonas maltophilia | Penicillin G | 397 | 0.035 | 1.1 × 10⁴ calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W204F, Penicillin G |
| metallo-beta-lactamase L1variant W206F | Stenotrophomonas maltophilia | Cephalothin | 49 | 0.014 | 3.5 × 10³ calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W206F, Cephalothin |
| metallo-beta-lactamase L1variant W206F | Stenotrophomonas maltophilia | Meropenem | 64 | 0.011 | 5.8 × 10³ calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W206F, Meropenem |
| metallo-beta-lactamase L1variant W206F | Stenotrophomonas maltophilia | Nitrocefin | 36 | 0.004 | 9.0 × 10³ calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W206F, Nitrocefin |
| metallo-beta-lactamase L1variant W206F | Stenotrophomonas maltophilia | Penicillin G | 407 | 0.031 | 1.3 × 10⁴ calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W206F, Penicillin G |
| metallo-beta-lactamase L1variant W269F | Stenotrophomonas maltophilia | Cephalothin | 55 | 0.0064 | 8.6 × 10³ calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W269F, Cephalothin |
| metallo-beta-lactamase L1variant W269F | Stenotrophomonas maltophilia | Meropenem | 34 | 0.008 | 4.2 × 10³ calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W269F, Meropenem |
| metallo-beta-lactamase L1variant W269F | Stenotrophomonas maltophilia | Nitrocefin | 21 | 0.0021 | 1.0 × 10⁴ calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W269F, Nitrocefin |
| metallo-beta-lactamase L1variant W269F | Stenotrophomonas maltophilia | Penicillin G | 376 | 0.034 | 1.1 × 10⁴ calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W269F, Penicillin G |
| metallo-beta-lactamase L1variant W39F | Stenotrophomonas maltophilia | Cephalothin | 33 | 0.033 | 1.0 × 10³ calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W39F, Cephalothin |
| metallo-beta-lactamase L1variant W39F | Stenotrophomonas maltophilia | Meropenem | 33 | 0.009 | 3.7 × 10³ calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W39F, Meropenem |
| metallo-beta-lactamase L1variant W39F | Stenotrophomonas maltophilia | Nitrocefin | 38 | 0.018 | 2.1 × 10³ calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W39F, Nitrocefin |
| metallo-beta-lactamase L1variant W39F | Stenotrophomonas maltophilia | Penicillin G | 96 | 0.125 | 770 calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W39F, Penicillin G |
| metallo-beta-lactamase L1variant W39F/D160W | Stenotrophomonas maltophilia | Cephalothin | 23 | 0.095 | 240 calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W39F/D160W, Cephalothin |
| metallo-beta-lactamase L1variant W39F/D160W | Stenotrophomonas maltophilia | Meropenem | 17 | 0.006 | 2.8 × 10³ calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W39F/D160W, Meropenem |
| metallo-beta-lactamase L1variant W39F/D160W | Stenotrophomonas maltophilia | Nitrocefin | 39 | 0.023 | 1.7 × 10³ calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W39F/D160W, Nitrocefin |
| metallo-beta-lactamase L1variant W39F/D160W | Stenotrophomonas maltophilia | Penicillin G | 161 | 0.356 | 452 calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W39F/D160W, Penicillin G |
| metallo-beta-lactamase L1variant W53F | Stenotrophomonas maltophilia | Cephalothin | 53 | 0.0049 | 1.1 × 10⁴ calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W53F, Cephalothin |
| metallo-beta-lactamase L1variant W53F | Stenotrophomonas maltophilia | Meropenem | 76 | 0.012 | 6.3 × 10³ calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W53F, Meropenem |
| metallo-beta-lactamase L1variant W53F | Stenotrophomonas maltophilia | Nitrocefin | 36 | 0.0039 | 9.2 × 10³ calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W53F, Nitrocefin |
| metallo-beta-lactamase L1variant W53F | Stenotrophomonas maltophilia | Penicillin G | 402 | 0.024 | 1.7 × 10⁴ calc. | pH 7, 25 °C | Open record: metallo-beta-lactamase L1 variant W53F, Penicillin G |