Values CatLog read from this paper
Domain-domain interactions in the aminoglycoside antibiotic resistance enzyme AAC(6')-APH(2'')
PMID 15274639doi:10.1021/bi049135yOpen in Browse
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CatLog read 29 records (87 values) from this paper: AAC(6′)-APH(2′′).
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| AAC(6′)-APH(2′′) | — | acetyl coenzyme AN-terminal His6-tagged | 0.19 | 0.247 | 0.76 | Open record: AAC(6′)-APH(2′′), acetyl coenzyme A | |
| AAC(6′)-APH(2′′) | — | acetyl coenzyme Auntagged | 0.34 | 0.0744 | 4.6 | Open record: AAC(6′)-APH(2′′), acetyl coenzyme A | |
| AAC(6′)-APH(2′′) | — | amikacinN-terminal His6-tagged; full-length | 0.27 | 0.0971 | 2.8 | Open record: AAC(6′)-APH(2′′), amikacin | |
| AAC(6′)-APH(2′′) | — | amikacinN-terminal His6-tagged; residues 1-194 | 0.01 | 0.11 | 0.094 | Open record: AAC(6′)-APH(2′′), amikacin | |
| AAC(6′)-APH(2′′) | — | amikacinresidues 1-194; untagged | 0.06 | 0.214 | 0.28 | Open record: AAC(6′)-APH(2′′), amikacin | |
| AAC(6′)-APH(2′′) | — | butirosinN-terminal His6-tagged; full-length | 0.13 | 0.0609 | 2.1 | Open record: AAC(6′)-APH(2′′), butirosin | |
| AAC(6′)-APH(2′′) | — | butirosinN-terminal His6-tagged; residues 1-194 | 0.002 | 0.0292 | 0.064 | Open record: AAC(6′)-APH(2′′), butirosin | |
| AAC(6′)-APH(2′′) | — | butirosinresidues 1-194; untagged | 0.01 | 0.383 | 0.035 | Open record: AAC(6′)-APH(2′′), butirosin | |
| AAC(6′)-APH(2′′) | — | fortimicin AN-terminal His6-tagged | 0.2 | 0.094 | 2.1 | Open record: AAC(6′)-APH(2′′), fortimicin A | |
| AAC(6′)-APH(2′′) | — | fortimicin Auntagged | 1.5 | 0.105 | 14 | Open record: AAC(6′)-APH(2′′), fortimicin A | |
| AAC(6′)-APH(2′′) | — | guanosine 5′-triphosphateN-terminal His6-tagged; full-length | 0.13 | 0.00374 | 36 | Open record: AAC(6′)-APH(2′′), guanosine 5′-triphosphate | |
| AAC(6′)-APH(2′′) | — | guanosine 5′-triphosphateresidues 175-479; untagged | 0.08 | 0.00787 | 10 | Open record: AAC(6′)-APH(2′′), guanosine 5′-triphosphate | |
| AAC(6′)-APH(2′′) | — | kanamycin AN-terminal His6-tagged | 0.15 | 0.233 | 0.65 | Open record: AAC(6′)-APH(2′′), kanamycin A | |
| AAC(6′)-APH(2′′) | — | kanamycin Auntagged | 0.38 | 0.146 | 2.6 | Open record: AAC(6′)-APH(2′′), kanamycin A | |
| AAC(6′)-APH(2′′) | — | ribostamycinN-terminal His6-tagged; full-length | 0.37 | 0.131 | 2.8 | Open record: AAC(6′)-APH(2′′), ribostamycin | |
| AAC(6′)-APH(2′′) | — | ribostamycinN-terminal His6-tagged; residues 1-194 | 0.01 | 0.0287 | 0.21 | Open record: AAC(6′)-APH(2′′), ribostamycin | |
| AAC(6′)-APH(2′′) | — | ribostamycinresidues 1-194; untagged | 0.04 | 0.12 | 0.3 | Open record: AAC(6′)-APH(2′′), ribostamycin | |
| AAC(6′)-APH(2′′)variant K190P | — | acetyl coenzyme A | 0.13 | 0.0738 | 1.7 | Open record: AAC(6′)-APH(2′′) variant K190P, acetyl coenzyme A | |
| AAC(6′)-APH(2′′)variant K190P | — | guanosine 5′-triphosphate | 0.03 | 0.00638 | 5 | Open record: AAC(6′)-APH(2′′) variant K190P, guanosine 5′-triphosphate | |
| AAC(6′)-APH(2′′)variant K190P | — | kanamycin Aacetyltransferase activity (aminoglycoside 6′-N-acetylation) | 0.21 | 0.0461 | 4.7 | Open record: AAC(6′)-APH(2′′) variant K190P, kanamycin A | |
| AAC(6′)-APH(2′′)variant K190P | — | kanamycin Acoupled assay: pyruvate kinase/lactate dehydrogenase; 48 | 0.03 | 0.00328 | 8.4 | Open record: AAC(6′)-APH(2′′) variant K190P, kanamycin A | |
| AAC(6′)-APH(2′′)variant K190P/L192P | — | acetyl coenzyme A | 0.01 | 0.0451 | 0.24 | Open record: AAC(6′)-APH(2′′) variant K190P/L192P, acetyl coenzyme A | |
| AAC(6′)-APH(2′′)variant K190P/L192P | — | guanosine 5′-triphosphate | 0.01 | 0.00355 | 3.3 | Open record: AAC(6′)-APH(2′′) variant K190P/L192P, guanosine 5′-triphosphate | |
| AAC(6′)-APH(2′′)variant K190P/L192P | — | kanamycin Aacetyltransferase activity (aminoglycoside 6′-N-acetylation) | 0.01 | 0.0365 | 0.34 | Open record: AAC(6′)-APH(2′′) variant K190P/L192P, kanamycin A | |
| AAC(6′)-APH(2′′)variant K190P/L192P | — | kanamycin Acoupled assay: pyruvate kinase/lactate dehydrogenase; 48 | 0.01 | 0.00266 | 4.5 | Open record: AAC(6′)-APH(2′′) variant K190P/L192P, kanamycin A | |
| AAC(6′)-APH(2′′)variant L192P | — | acetyl coenzyme A | 0.01 | 0.0213 | 0.64 | Open record: AAC(6′)-APH(2′′) variant L192P, acetyl coenzyme A | |
| AAC(6′)-APH(2′′)variant L192P | — | guanosine 5′-triphosphate | 0.03 | 0.00314 | 10 | Open record: AAC(6′)-APH(2′′) variant L192P, guanosine 5′-triphosphate | |
| AAC(6′)-APH(2′′)variant L192P | — | kanamycin Aacetyltransferase activity (aminoglycoside 6′-N-acetylation) | 0.03 | 0.0368 | 0.67 | Open record: AAC(6′)-APH(2′′) variant L192P, kanamycin A | |
| AAC(6′)-APH(2′′)variant L192P | — | kanamycin Acoupled assay: pyruvate kinase/lactate dehydrogenase; 48 | 0.03 | 4 × 10⁻⁴ | 74 | Open record: AAC(6′)-APH(2′′) variant L192P, kanamycin A |