Values CatLog read from this paper
Mechanism of cobyrinic acid a,c-diamide synthetase from Salmonella typhimurium LT2
PMID 15311923doi:10.1021/bi048972xOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 43 records (77 values) from this paper: cobyrinic acid a,c-diamide synthetase (CbiA) from Salmonella typhimurium.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 14 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cobyrinic acid a,c-diamide synthetase (CbiA) | Salmonella typhimurium | adenosine 5'-triphosphate | 0.13 | 0.0027 | 48.2 | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA), adenosine 5'-triphosphate |
| cobyrinic acid a,c-diamide synthetase (CbiA) | Salmonella typhimurium | ammonia | 0.18 | 26.2 | 0.0069 | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA), ammonia |
| cobyrinic acid a,c-diamide synthetase (CbiA) | Salmonella typhimurium | cobyrinic acidoverall synthetase reaction; rate of ADP formation | 0.16 | 7.4 × 10⁻⁴ | 216 | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA), cobyrinic acid |
| cobyrinic acid a,c-diamide synthetase (CbiA) | Salmonella typhimurium | cobyrinic acidamidation (synthetase) reaction followed by HPLC time course | 0.11 | 2 × 10⁻⁴ | 550 calc. | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA), cobyrinic acid |
| cobyrinic acid a,c-diamide synthetase (CbiA) | Salmonella typhimurium | cobyrinic acid c-monoamide | 0.2 | 4.1 × 10⁻⁴ | 490 calc. | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA), cobyrinic acid c-monoamide |
| cobyrinic acid a,c-diamide synthetase (CbiA) | Salmonella typhimurium | glutamineoverall synthetase reaction; rate of ADP formation; Table 1 | 0.16 | 0.053 | 3.04 | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA), glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA) | Salmonella typhimurium | glutamineglutaminase partial reaction (glutamine hydrolysis); Table 2 | 1.2 | 49 | 0.024 | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA), glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA) | Salmonella typhimurium | glutamineglutaminase partial reaction (glutamine hydrolysis); Table 2 | 1.2 | 25 | 0.048 | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA), glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA) | Salmonella typhimurium | glutamineglutaminase partial reaction (glutamine hydrolysis); Table 2 | 2.6 | 2.6 | 1 | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA), glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA) | Salmonella typhimurium | glutamineglutaminase partial reaction (glutamine hydrolysis); Table 2 | 1.2 | 0.18 | 6.67 | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA), glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA) | Salmonella typhimurium | glutamineglutaminase partial reaction (glutamine hydrolysis); Table 2 | 1.5 | 3.2 | 0.468 | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA), glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA) | Salmonella typhimurium | glutamineglutaminase partial reaction (glutamine hydrolysis); Table 2 | 1.3 | 1.8 | 0.722 | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA), glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA) | Salmonella typhimurium | glutamineglutaminase partial reaction (glutamine hydrolysis); Table 4 | — | 0.2 | — | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA), glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant D45N | Salmonella typhimurium | glutamineno ATP or cobyrinic acid | 1.3 | 34 | 0.038 calc. | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant D45N, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant D45N | Salmonella typhimurium | glutamine2.0 mM ATP | — | 53 | — | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant D45N, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant D45N | Salmonella typhimurium | glutamine12.5 µM cobyrinic acid | — | 3.2 | — | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant D45N, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant D45N | Salmonella typhimurium | glutamine2.0 mM ATP + 12.5 µM cobyrinic acid | — | 6.1 | — | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant D45N, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant D48N | Salmonella typhimurium | glutamineno ATP or cobyrinic acid | 1 | 38 | 0.026 calc. | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant D48N, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant D48N | Salmonella typhimurium | glutamine2.0 mM ATP | — | 45 | — | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant D48N, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant D48N | Salmonella typhimurium | glutamine12.5 µM cobyrinic acid | — | 3.7 | — | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant D48N, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant D48N | Salmonella typhimurium | glutamine2.0 mM ATP + 12.5 µM cobyrinic acid | — | 4.4 | — | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant D48N, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant D97N | Salmonella typhimurium | cobyrinic acid | 0.03 | 4.1 × 10⁻⁴ | 73 calc. | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant D97N, cobyrinic acid |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant D97N | Salmonella typhimurium | cobyrinic acid c-monoamide | 0.04 | 3 × 10⁻⁴ | 130 calc. | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant D97N, cobyrinic acid c-monoamide |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant D97N | Salmonella typhimurium | glutamineno ATP or cobyrinic acid | 1 | 49 | 0.020 calc. | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant D97N, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant D97N | Salmonella typhimurium | glutamine2.0 mM ATP | — | 22 | — | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant D97N, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant D97N | Salmonella typhimurium | glutamine12.5 µM cobyrinic acid | — | 19 | — | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant D97N, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant D97N | Salmonella typhimurium | glutamine2.0 mM ATP + 12.5 µM cobyrinic acid | — | 12 | — | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant D97N, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant E90Q | Salmonella typhimurium | glutamineno ATP or cobyrinic acid | 0.8 | 20 | 0.040 calc. | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant E90Q, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant E90Q | Salmonella typhimurium | glutamine2.0 mM ATP | — | 31 | — | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant E90Q, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant E90Q | Salmonella typhimurium | glutamine12.5 µM cobyrinic acid | — | 3.7 | — | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant E90Q, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant E90Q | Salmonella typhimurium | glutamine2.0 mM ATP + 12.5 µM cobyrinic acid | — | 9.6 | — | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant E90Q, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant L47A | Salmonella typhimurium | cobyrinic acid | 0.014 | 4.1 × 10⁻⁴ | 34 calc. | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant L47A, cobyrinic acid |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant L47A | Salmonella typhimurium | cobyrinic acid c-monoamide | 0.057 | 0.0027 | 21 calc. | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant L47A, cobyrinic acid c-monoamide |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant L47A | Salmonella typhimurium | glutamineno ATP or cobyrinic acid | 1.3 | 38 | 0.034 calc. | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant L47A, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant L47A | Salmonella typhimurium | glutamine2.0 mM ATP | — | 33 | — | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant L47A, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant L47A | Salmonella typhimurium | glutamine12.5 µM cobyrinic acid | — | 20 | — | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant L47A, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant L47A | Salmonella typhimurium | glutamine2.0 mM ATP + 12.5 µM cobyrinic acid | — | 5 | — | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant L47A, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant Y46A | Salmonella typhimurium | cobyrinic acid | 0.044 | 4.4 × 10⁻⁴ | 100 calc. | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant Y46A, cobyrinic acid |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant Y46A | Salmonella typhimurium | cobyrinic acid c-monoamide | 0.008 | 0.011 | 0.73 calc. | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant Y46A, cobyrinic acid c-monoamide |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant Y46A | Salmonella typhimurium | glutamineno ATP or cobyrinic acid | 0.75 | 66 | 0.011 calc. | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant Y46A, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant Y46A | Salmonella typhimurium | glutamine2.0 mM ATP | — | 75 | — | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant Y46A, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant Y46A | Salmonella typhimurium | glutamine12.5 µM cobyrinic acid | — | 64 | — | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant Y46A, glutamine |
| cobyrinic acid a,c-diamide synthetase (CbiA)variant Y46A | Salmonella typhimurium | glutamine2.0 mM ATP + 12.5 µM cobyrinic acid | — | 21 | — | pH 7.7, 30 °C | Open record: cobyrinic acid a,c-diamide synthetase (CbiA) variant Y46A, glutamine |