Values CatLog read from this paper
Site-saturation mutagenesis of Tyr-105 reveals its importance in substrate stabilization and discrimination in TEM-1 beta-lactamase
PMID 15326193doi:10.1074/jbc.m407606200Open in Browse
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CatLog read 12 records (36 values) from this paper: TEM-1 beta-lactamase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| TEM-1 beta-lactamase | Escherichia coli | benzylpenicillin | 1.24 × 10³ | 0.043 | 2.9 × 10⁴ | pH 7 | Open record: TEM-1 beta-lactamase, benzylpenicillin |
| TEM-1 beta-lactamase | Escherichia coli | cephalothin | 105 | 0.177 | 590 | pH 7 | Open record: TEM-1 beta-lactamase, cephalothin |
| TEM-1 beta-lactamasevariant Y105D | Escherichia coli | benzylpenicillin | 255 | 0.369 | 690 | pH 7 | Open record: TEM-1 beta-lactamase variant Y105D, benzylpenicillin |
| TEM-1 beta-lactamasevariant Y105D | Escherichia coli | cephalothin | 51 | 2.86 | 18 | pH 7 | Open record: TEM-1 beta-lactamase variant Y105D, cephalothin |
| TEM-1 beta-lactamasevariant Y105G | Escherichia coli | benzylpenicillin | 1203 | 0.152 | 7.9 × 10³ | pH 7 | Open record: TEM-1 beta-lactamase variant Y105G, benzylpenicillin |
| TEM-1 beta-lactamasevariant Y105G | Escherichia coli | cephalothin | 45 | 1.63 | 28 | pH 7 | Open record: TEM-1 beta-lactamase variant Y105G, cephalothin |
| TEM-1 beta-lactamasevariant Y105N | Escherichia coli | benzylpenicillin | 1616 | 0.276 | 5.9 × 10³ | pH 7 | Open record: TEM-1 beta-lactamase variant Y105N, benzylpenicillin |
| TEM-1 beta-lactamasevariant Y105N | Escherichia coli | cephalothin | 61 | 0.303 | 200 | pH 7 | Open record: TEM-1 beta-lactamase variant Y105N, cephalothin |
| TEM-1 beta-lactamasevariant Y105R | Escherichia coli | benzylpenicillin | 525 | 0.156 | 3.4 × 10³ | pH 7 | Open record: TEM-1 beta-lactamase variant Y105R, benzylpenicillin |
| TEM-1 beta-lactamasevariant Y105R | Escherichia coli | cephalothin | 90 | 0.546 | 160 | pH 7 | Open record: TEM-1 beta-lactamase variant Y105R, cephalothin |
| TEM-1 beta-lactamasevariant Y105W | Escherichia coli | benzylpenicillin | 900 | 0.023 | 3.9 × 10⁴ | pH 7 | Open record: TEM-1 beta-lactamase variant Y105W, benzylpenicillin |
| TEM-1 beta-lactamasevariant Y105W | Escherichia coli | cephalothin | 65 | 0.074 | 880 | pH 7 | Open record: TEM-1 beta-lactamase variant Y105W, cephalothin |