Values CatLog read from this paper
The role of the conserved residues His-246, His-199, and Tyr-255 in the catalysis of catechol 2,3-dioxygenase from Pseudomonas stutzeri OX1
PMID 15347689doi:10.1074/jbc.m406243200Open in Browse
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CatLog read 15 records (43 values) from this paper: catechol 2,3-dioxygenase (C2,3O) from Pseudomonas stutzeri.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| catechol 2,3-dioxygenase (C2,3O) | Pseudomonas stutzeri | 2,3-dihydroxybenzaldehyde | 47 | 0.032 | 1.5 × 10³ | pH 7.5, 25 °C | Open record: catechol 2,3-dioxygenase (C2,3O), 2,3-dihydroxybenzaldehyde |
| catechol 2,3-dioxygenase (C2,3O) | Pseudomonas stutzeri | 3,4-dihydroxybenzaldehyde | 16 | 0.188 | 85 | pH 7.5, 25 °C | Open record: catechol 2,3-dioxygenase (C2,3O), 3,4-dihydroxybenzaldehyde |
| catechol 2,3-dioxygenase (C2,3O) | Pseudomonas stutzeri | 3-methylcatechol | — | 0.0038 | — | pH 7.5, 25 °C | Open record: catechol 2,3-dioxygenase (C2,3O), 3-methylcatechol |
| catechol 2,3-dioxygenase (C2,3O) | Pseudomonas stutzeri | 4-nitrocatechol | 1.4 | 0.035 | 40 | pH 7.5, 25 °C | Open record: catechol 2,3-dioxygenase (C2,3O), 4-nitrocatechol |
| catechol 2,3-dioxygenase (C2,3O) | Pseudomonas stutzeri | catechol | 200 | 0.0015 | 1.2 × 10⁵ | pH 7.5, 25 °C | Open record: catechol 2,3-dioxygenase (C2,3O), catechol |
| catechol 2,3-dioxygenase (C2,3O)variant H199N | Pseudomonas stutzeri | 2,3-dihydroxybenzaldehyde | 0.03 | 1.17 | 0.026 | pH 7.5, 25 °C | Open record: catechol 2,3-dioxygenase (C2,3O) variant H199N, 2,3-dihydroxybenzaldehyde |
| catechol 2,3-dioxygenase (C2,3O)variant H199N | Pseudomonas stutzeri | 3,4-dihydroxybenzaldehyde | 0.0047 | 0.6 | 0.0078 | pH 7.5, 25 °C | Open record: catechol 2,3-dioxygenase (C2,3O) variant H199N, 3,4-dihydroxybenzaldehyde |
| catechol 2,3-dioxygenase (C2,3O)variant H199N | Pseudomonas stutzeri | catechol | 0.46 | 0.434 | 1.1 | pH 7.5, 25 °C | Open record: catechol 2,3-dioxygenase (C2,3O) variant H199N, catechol |
| catechol 2,3-dioxygenase (C2,3O)variant H246A | Pseudomonas stutzeri | catechol | 0.45 | 5 | 0.09 | pH 7.5, 25 °C | Open record: catechol 2,3-dioxygenase (C2,3O) variant H246A, catechol |
| catechol 2,3-dioxygenase (C2,3O)variant H246N | Pseudomonas stutzeri | 2,3-dihydroxybenzaldehyde | 6.9 | 0.44 | 16 | pH 7.5, 25 °C | Open record: catechol 2,3-dioxygenase (C2,3O) variant H246N, 2,3-dihydroxybenzaldehyde |
| catechol 2,3-dioxygenase (C2,3O)variant H246N | Pseudomonas stutzeri | 3,4-dihydroxybenzaldehyde | 0.43 | 0.97 | 0.44 | pH 7.5, 25 °C | Open record: catechol 2,3-dioxygenase (C2,3O) variant H246N, 3,4-dihydroxybenzaldehyde |
| catechol 2,3-dioxygenase (C2,3O)variant H246N | Pseudomonas stutzeri | catechol | 1.6 | 0.714 | 2.2 | pH 7.5, 25 °C | Open record: catechol 2,3-dioxygenase (C2,3O) variant H246N, catechol |
| catechol 2,3-dioxygenase (C2,3O)variant Y255F | Pseudomonas stutzeri | 2,3-dihydroxybenzaldehyde | 2.6 | 0.0516 | 50 | pH 7.5, 25 °C | Open record: catechol 2,3-dioxygenase (C2,3O) variant Y255F, 2,3-dihydroxybenzaldehyde |
| catechol 2,3-dioxygenase (C2,3O)variant Y255F | Pseudomonas stutzeri | 3,4-dihydroxybenzaldehyde | 0.45 | 0.248 | 1.8 | pH 7.5, 25 °C | Open record: catechol 2,3-dioxygenase (C2,3O) variant Y255F, 3,4-dihydroxybenzaldehyde |
| catechol 2,3-dioxygenase (C2,3O)variant Y255F | Pseudomonas stutzeri | catechol | 8.8 | 0.005 | 1.76 × 10³ | pH 7.5, 25 °C | Open record: catechol 2,3-dioxygenase (C2,3O) variant Y255F, catechol |