Values CatLog read from this paper
Characterization of recombinant rat cathepsin B and nonglycosylated mutants expressed in yeast. New insights into the pH dependence of cathepsin B-catalyzed hydrolyses
PMID 1537854doi:10.1016/s0021-9258(18)42891-8Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 43 records (77 values) from this paper: cathepsin B from Rattus norvegicus.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 4 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| cathepsin B | Rattus norvegicus | N-benzyloxycarbonyl-L-arginyl-L-arginine 7-amido-4-methylcoumarin | 57.9 | 1.27 | 46.37 | pH 6 | Open record: cathepsin B, N-benzyloxycarbonyl-L-arginyl-L-arginine 7-amido-4-methylcoumarin |
| cathepsin B | Rattus norvegicus | N-benzyloxycarbonyl-L-arginyl-L-arginine 7-amido-4-methylcoumarin | — | — | 44.73 | 24 °C | Open record: cathepsin B, N-benzyloxycarbonyl-L-arginyl-L-arginine 7-amido-4-methylcoumarin |
| cathepsin B | Rattus norvegicus | N-benzyloxycarbonyl-L-arginyl-L-arginine 7-amido-4-methylcoumarin | — | — | 115.9 | 24 °C | Open record: cathepsin B, N-benzyloxycarbonyl-L-arginyl-L-arginine 7-amido-4-methylcoumarin |
| cathepsin B | Rattus norvegicus | N-benzyloxycarbonyl-L-arginyl-L-arginine 7-amido-4-methylcoumarin | — | — | 53.7 | 24 °C | Open record: cathepsin B, N-benzyloxycarbonyl-L-arginyl-L-arginine 7-amido-4-methylcoumarin |
| cathepsin B | Rattus norvegicus | N-benzyloxycarbonyl-L-arginyl-L-arginine p-nitroanilideSephadex G-50 pooled fraction A | 2.71 | 0.53 | 5.4 | pH 6, 24 °C | Open record: cathepsin B, N-benzyloxycarbonyl-L-arginyl-L-arginine p-nitroanilide |
| cathepsin B | Rattus norvegicus | N-benzyloxycarbonyl-L-arginyl-L-arginine p-nitroanilideSephadex G-50 pooled fraction B | 5.57 | 0.48 | 11.8 | pH 6, 24 °C | Open record: cathepsin B, N-benzyloxycarbonyl-L-arginyl-L-arginine p-nitroanilide |
| cathepsin B | Rattus norvegicus | N-benzyloxycarbonyl-L-arginyl-L-arginine p-nitroanilideSephadex G-50 pooled fraction C | 7.08 | 0.44 | 16.8 | pH 6, 24 °C | Open record: cathepsin B, N-benzyloxycarbonyl-L-arginyl-L-arginine p-nitroanilide |
| cathepsin B | Rattus norvegicus | N-benzyloxycarbonyl-L-arginyl-L-arginine p-nitroanilide | 8.29 | 1.01 | 8.31 | pH 6 | Open record: cathepsin B, N-benzyloxycarbonyl-L-arginyl-L-arginine p-nitroanilide |
| cathepsin B | Rattus norvegicus | N-benzyloxycarbonyl-L-arginyl-L-arginine p-nitroanilide | — | — | 6.737 | 24 °C | Open record: cathepsin B, N-benzyloxycarbonyl-L-arginyl-L-arginine p-nitroanilide |
| cathepsin B | Rattus norvegicus | N-benzyloxycarbonyl-L-arginyl-L-arginine p-nitroanilide | — | — | 76.04 | 24 °C | Open record: cathepsin B, N-benzyloxycarbonyl-L-arginyl-L-arginine p-nitroanilide |
| cathepsin B | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarinSephadex G-50 pooled fraction A | 1.38 | 0.72 | 1.86 | pH 6, 24 °C | Open record: cathepsin B, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin |
| cathepsin B | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarinSephadex G-50 pooled fraction B | 8.22 | 1.15 | 7.02 | pH 6, 24 °C | Open record: cathepsin B, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin |
| cathepsin B | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarinSephadex G-50 pooled fraction C | 9.96 | 0.87 | 11.1 | pH 6, 24 °C | Open record: cathepsin B, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin |
| cathepsin B | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin | 81.4 | 0.28 | 297.9 | pH 6 | Open record: cathepsin B, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin |
| cathepsin B | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin | — | — | 149.5 | 24 °C | Open record: cathepsin B, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin |
| cathepsin B | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin | — | — | 323.4 | 24 °C | Open record: cathepsin B, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin |
| cathepsin B | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin | — | — | 700.6 | 24 °C | Open record: cathepsin B, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin |
| cathepsin B | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin | — | — | 543.2 | 24 °C | Open record: cathepsin B, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin |
| cathepsin B | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine p-nitroanilide | 35.2 | 0.62 | 57.1 | pH 6 | Open record: cathepsin B, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine p-nitroanilide |
| cathepsin B | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine p-nitroanilide | — | — | 27.53 | 24 °C | Open record: cathepsin B, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine p-nitroanilide |
| cathepsin B | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine p-nitroanilide | — | — | 51.1 | 24 °C | Open record: cathepsin B, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine p-nitroanilide |
| cathepsin B | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine p-nitroanilide | — | — | 471 | 24 °C | Open record: cathepsin B, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine p-nitroanilide |
| cathepsin Bvariant S115A | Rattus norvegicus | N-benzyloxycarbonyl-L-arginyl-L-arginine p-nitroanilide | 8.55 | 0.99 | 8.6 | pH 6 | Open record: cathepsin B variant S115A, N-benzyloxycarbonyl-L-arginyl-L-arginine p-nitroanilide |
| cathepsin Bvariant S115A | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin | — | — | 183.9 | 24 °C | Open record: cathepsin B variant S115A, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin |
| cathepsin Bvariant S115A | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin | — | — | 334.3 | 24 °C | Open record: cathepsin B variant S115A, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin |
| cathepsin Bvariant S115A | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin | — | — | 806 | 24 °C | Open record: cathepsin B variant S115A, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin |
| cathepsin Bvariant S115A | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin | — | — | 372.6 | 24 °C | Open record: cathepsin B variant S115A, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin |
| cathepsin Bvariant S115A | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine p-nitroanilide | — | — | 52.2 | pH 6 | Open record: cathepsin B variant S115A, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine p-nitroanilide |
| cathepsin Bvariant S115A/Q255Term | Rattus norvegicus | N-benzyloxycarbonyl-L-arginyl-L-arginine 7-amido-4-methylcoumarin | 51.4 | 1.11 | 46.4 | pH 6 | Open record: cathepsin B variant S115A/Q255Term, N-benzyloxycarbonyl-L-arginyl-L-arginine 7-amido-4-methylcoumarin |
| cathepsin Bvariant S115A/Q255Term | Rattus norvegicus | N-benzyloxycarbonyl-L-arginyl-L-arginine p-nitroanilide | 9.67 | 1.19 | 8.11 | pH 6 | Open record: cathepsin B variant S115A/Q255Term, N-benzyloxycarbonyl-L-arginyl-L-arginine p-nitroanilide |
| cathepsin Bvariant S115A/Q255Term | Rattus norvegicus | N-benzyloxycarbonyl-L-arginyl-L-arginine p-nitroanilide | — | — | 6.908 | 24 °C | Open record: cathepsin B variant S115A/Q255Term, N-benzyloxycarbonyl-L-arginyl-L-arginine p-nitroanilide |
| cathepsin Bvariant S115A/Q255Term | Rattus norvegicus | N-benzyloxycarbonyl-L-arginyl-L-arginine p-nitroanilide | — | — | 44.24 | 24 °C | Open record: cathepsin B variant S115A/Q255Term, N-benzyloxycarbonyl-L-arginyl-L-arginine p-nitroanilide |
| cathepsin Bvariant S115A/Q255Term | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin | 77.1 | 0.23 | 335 | pH 6 | Open record: cathepsin B variant S115A/Q255Term, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin |
| cathepsin Bvariant S115A/Q255Term | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarinnucleophile competition with Gly-Gly (50-600 mM) | 75.9 | — | — | pH 7 | Open record: cathepsin B variant S115A/Q255Term, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin |
| cathepsin Bvariant S115A/Q255Term | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin | 80.8 | 0.132 | 612 calc. | pH 7 | Open record: cathepsin B variant S115A/Q255Term, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin |
| cathepsin Bvariant S115A/Q255Term | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarinnucleophile competition with Gly-Gly (50-600 mM) | 23.1 | — | — | pH 8.2 | Open record: cathepsin B variant S115A/Q255Term, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin |
| cathepsin Bvariant S115A/Q255Term | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin | 24.2 | 0.067 | 360 calc. | pH 8.2 | Open record: cathepsin B variant S115A/Q255Term, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine 7-amido-4-methylcoumarin |
| cathepsin Bvariant S115A/Q255Term | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine p-nitroanilide | 34.4 | 0.6 | 57.7 | pH 6 | Open record: cathepsin B variant S115A/Q255Term, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine p-nitroanilide |
| cathepsin Bvariant S115A/Q255Term | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine p-nitroanilide | 31.6 | 0.3 | 110 calc. | pH 7 | Open record: cathepsin B variant S115A/Q255Term, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine p-nitroanilide |
| cathepsin Bvariant S115A/Q255Term | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine p-nitroanilide | 23.1 | 0.117 | 197 calc. | pH 8.2 | Open record: cathepsin B variant S115A/Q255Term, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine p-nitroanilide |
| cathepsin Bvariant S115A/Q255Term | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine p-nitroanilide | — | — | 25.26 | 24 °C | Open record: cathepsin B variant S115A/Q255Term, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine p-nitroanilide |
| cathepsin Bvariant S115A/Q255Term | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine p-nitroanilide | — | — | 53.54 | 24 °C | Open record: cathepsin B variant S115A/Q255Term, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine p-nitroanilide |
| cathepsin Bvariant S115A/Q255Term | Rattus norvegicus | N-benzyloxycarbonyl-L-phenylalanyl-L-arginine p-nitroanilide | — | — | 314.3 | 24 °C | Open record: cathepsin B variant S115A/Q255Term, N-benzyloxycarbonyl-L-phenylalanyl-L-arginine p-nitroanilide |