Values CatLog read from this paper
Asparaginyl-tRNA synthetase from the Escherichia coli temperature-sensitive strain HO202. A proline replacement in motif 2 is responsible for a large increase in Km for asparagine and ATP
PMID 1544480doi:10.1016/0014-5793(92)80106-qOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 12 records (18 values) from this paper: asparaginyl-tRNA synthetase (AsnRS) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 6 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| asparaginyl-tRNA synthetase (AsnRS) | Escherichia coli | adenosine 5'-triphosphateATP-PPi exchange reaction; 5.5 mM [32P]PPi | — | 0.5 | — | pH 7.6, 37 °C | Open record: asparaginyl-tRNA synthetase (AsnRS), adenosine 5'-triphosphate |
| asparaginyl-tRNA synthetase (AsnRS) | Escherichia coli | adenosine 5'-triphosphateaminoacylation (tRNA charging) reaction; 0.2 mM spermidine | 1.3 | 0.076 | 17 calc. | pH 7.6, 37 °C | Open record: asparaginyl-tRNA synthetase (AsnRS), adenosine 5'-triphosphate |
| asparaginyl-tRNA synthetase (AsnRS) | Escherichia coli | L-asparagineATP-PPi exchange reaction; 5.5 mM [32P]PPi | — | 0.015 | — | pH 7.6, 37 °C | Open record: asparaginyl-tRNA synthetase (AsnRS), L-asparagine |
| asparaginyl-tRNA synthetase (AsnRS) | Escherichia coli | L-asparagine | 2.8 | 0.029 | 97 calc. | pH 7.6, 25 °C | Open record: asparaginyl-tRNA synthetase (AsnRS), L-asparagine |
| asparaginyl-tRNA synthetase (AsnRS) | Escherichia coli | L-asparagineaminoacylation (tRNA charging) reaction; 0.2 mM spermidine | 1.6 | 0.032 | 50 calc. | pH 7.6, 37 °C | Open record: asparaginyl-tRNA synthetase (AsnRS), L-asparagine |
| asparaginyl-tRNA synthetase (AsnRS)variant P231L | Escherichia coli | adenosine 5'-triphosphate | — | 0.87 | — | pH 7.6, 25 °C | Open record: asparaginyl-tRNA synthetase (AsnRS) variant P231L, adenosine 5'-triphosphate |
| asparaginyl-tRNA synthetase (AsnRS)variant P231L | Escherichia coli | adenosine 5'-triphosphateATP-PPi exchange reaction; 5.5 mM [32P]PPi | — | 4 | — | pH 7.6, 37 °C | Open record: asparaginyl-tRNA synthetase (AsnRS) variant P231L, adenosine 5'-triphosphate |
| asparaginyl-tRNA synthetase (AsnRS)variant P231L | Escherichia coli | adenosine 5'-triphosphateaminoacylation (tRNA charging) reaction; 0.2 mM spermidine | 0.3 | 1 | 0.30 calc. | pH 7.6, 37 °C | Open record: asparaginyl-tRNA synthetase (AsnRS) variant P231L, adenosine 5'-triphosphate |
| asparaginyl-tRNA synthetase (AsnRS)variant P231L | Escherichia coli | L-asparagineATP-PPi exchange reaction; 5.5 mM [32P]PPi | — | 0.094 | — | pH 7.6, 25 °C | Open record: asparaginyl-tRNA synthetase (AsnRS) variant P231L, L-asparagine |
| asparaginyl-tRNA synthetase (AsnRS)variant P231L | Escherichia coli | L-asparagineATP-PPi exchange reaction; 5.5 mM [32P]PPi | — | 0.77 | — | pH 7.6, 37 °C | Open record: asparaginyl-tRNA synthetase (AsnRS) variant P231L, L-asparagine |
| asparaginyl-tRNA synthetase (AsnRS)variant P231L | Escherichia coli | L-asparagineaminoacylation (tRNA charging) reaction; 0.2 mM spermidine | 2.2 | 0.09 | 24 calc. | pH 7.6, 25 °C | Open record: asparaginyl-tRNA synthetase (AsnRS) variant P231L, L-asparagine |
| asparaginyl-tRNA synthetase (AsnRS)variant P231L | Escherichia coli | L-asparagineaminoacylation (tRNA charging) reaction; 0.2 mM spermidine | 0.9 | 0.33 | 2.7 calc. | pH 7.6, 37 °C | Open record: asparaginyl-tRNA synthetase (AsnRS) variant P231L, L-asparagine |