CatLogenzyme kinetics catalog

Values CatLog read from this paper

Narrowing substrate specificity in a directly evolved enzyme: the A293D mutant of aspartate aminotransferase

PMID 15461450doi:10.1021/bi0487544Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 5 records (11 values) from this paper: aspartate aminotransferase (eAATase) from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.6.1.1

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
aspartate aminotransferase (eAATase)variant A12T/P13T/N34D/T109S/G261A/S285G/A293D/N297SEscherichia colialanine——0.0696pH 8Open
aspartate aminotransferase (eAATase)variant A12T/P13T/N34D/T109S/G261A/S285G/A293D/N297SEscherichia coliaspartate43192.2pH 8Open
aspartate aminotransferase (eAATase)variant A12T/P13T/N34D/T109S/G261A/S285G/A293D/N297SEscherichia coliphenylalanine100.334pH 8Open
aspartate aminotransferase (eAATase)variant A12T/P13T/N34D/T109S/G261A/S285G/A293D/N297SEscherichia coliα-ketoglutaratecoupled assay: HO-HxoDH (2-hydroxyisocaproate dehydrogenase)—0.4821pH 8Open
aspartate aminotransferase (eAATase)variant A12T/P13T/N34D/T109S/G261A/S285G/A293D/N297SEscherichia coliα-ketoglutaratecoupled assay: MDH (malate dehydrogenase); cosubstrate Asp—2.616pH 8Open