Values CatLog read from this paper
Structure-based engineering of Alcaligenes xylosoxidans copper-containing nitrite reductase enhances intermolecular electron transfer reaction with pseudoazurin
PMID 15475344doi:10.1074/jbc.m410198200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 9 records (18 values) from this paper: copper-containing nitrite reductase (AxNIR), copper-containing nitrite reductase (AcNIR) from Achromobacter xylosoxidans, Achromobacter cycloclastes.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 9 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| copper-containing nitrite reductase (AcNIR) | Achromobacter cycloclastes | reduced pseudoazurin from Achromobacter cycloclastes IAM1013 | 163 | 0.02 | 8.2 × 10³ calc. | pH 7, 25 °C | Open record: copper-containing nitrite reductase (AcNIR), reduced pseudoazurin from Achromobacter cycloclastes IAM1013 |
| copper-containing nitrite reductase (AxNIR) | Achromobacter xylosoxidans | reduced pseudoazurin from Achromobacter cycloclastes IAM1013 | 172 | 1.34 | 128 calc. | pH 7, 25 °C | Open record: copper-containing nitrite reductase (AxNIR), reduced pseudoazurin from Achromobacter cycloclastes IAM1013 |
| copper-containing nitrite reductase (AxNIR)variant A191E | Achromobacter xylosoxidans | reduced pseudoazurin from Achromobacter cycloclastes IAM1013 | 185 | 0.96 | 190 calc. | pH 7, 25 °C | Open record: copper-containing nitrite reductase (AxNIR) variant A191E, reduced pseudoazurin from Achromobacter cycloclastes IAM1013 |
| copper-containing nitrite reductase (AxNIR)variant A191E/G198E | Achromobacter xylosoxidans | reduced pseudoazurin from Achromobacter cycloclastes IAM1013 | 165 | 0.24 | 690 calc. | pH 7, 25 °C | Open record: copper-containing nitrite reductase (AxNIR) variant A191E/G198E, reduced pseudoazurin from Achromobacter cycloclastes IAM1013 |
| copper-containing nitrite reductase (AxNIR)variant A83D | Achromobacter xylosoxidans | reduced pseudoazurin from Achromobacter cycloclastes IAM1013 | 193 | 1.01 | 191 calc. | pH 7, 25 °C | Open record: copper-containing nitrite reductase (AxNIR) variant A83D, reduced pseudoazurin from Achromobacter cycloclastes IAM1013 |
| copper-containing nitrite reductase (AxNIR)variant A83D/A191E | Achromobacter xylosoxidans | reduced pseudoazurin from Achromobacter cycloclastes IAM1013 | 193 | 0.32 | 600 calc. | pH 7, 25 °C | Open record: copper-containing nitrite reductase (AxNIR) variant A83D/A191E, reduced pseudoazurin from Achromobacter cycloclastes IAM1013 |
| copper-containing nitrite reductase (AxNIR)variant A83D/A191E/G198E | Achromobacter xylosoxidans | reduced pseudoazurin from Achromobacter cycloclastes IAM1013 | 157 | 0.088 | 1.8 × 10³ calc. | pH 7, 25 °C | Open record: copper-containing nitrite reductase (AxNIR) variant A83D/A191E/G198E, reduced pseudoazurin from Achromobacter cycloclastes IAM1013 |
| copper-containing nitrite reductase (AxNIR)variant A83D/G198E | Achromobacter xylosoxidans | reduced pseudoazurin from Achromobacter cycloclastes IAM1013 | 198 | 0.25 | 790 calc. | pH 7, 25 °C | Open record: copper-containing nitrite reductase (AxNIR) variant A83D/G198E, reduced pseudoazurin from Achromobacter cycloclastes IAM1013 |
| copper-containing nitrite reductase (AxNIR)variant G198E | Achromobacter xylosoxidans | reduced pseudoazurin from Achromobacter cycloclastes IAM1013 | 167 | 0.49 | 340 calc. | pH 7, 25 °C | Open record: copper-containing nitrite reductase (AxNIR) variant G198E, reduced pseudoazurin from Achromobacter cycloclastes IAM1013 |