Values CatLog read from this paper
Phosphoenolpyruvate- and ATP-dependent dihydroxyacetone kinases: covalent substrate-binding and kinetic mechanism
PMID 15476397doi:10.1021/bi048575mOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 17 records (33 values) from this paper: ATP-dependent dihydroxyacetone kinase, PTS-dependent dihydroxyacetone kinase DhaK/DhaL/DhaM, PTS-dependent dihydroxyacetone kinase DhaK-DhaL complex from Citrobacter freundii, Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| ATP-dependent dihydroxyacetone kinase | Citrobacter freundii | adenosine 5'-triphosphatebisubstrate kinetics; D,L-GA fixed at 0.500 mM | 5 | 0.13 | 38 | pH 7.5, 30 °C | Open record: ATP-dependent dihydroxyacetone kinase, adenosine 5'-triphosphate |
| ATP-dependent dihydroxyacetone kinase | Citrobacter freundii | adenosine 5'-triphosphatebisubstrate kinetics; D,L-GA fixed at 0.250 mM | 4.2 | 0.11 | 38 | pH 7.5, 30 °C | Open record: ATP-dependent dihydroxyacetone kinase, adenosine 5'-triphosphate |
| ATP-dependent dihydroxyacetone kinase | Citrobacter freundii | adenosine 5'-triphosphatebisubstrate kinetics; D,L-GA fixed at 0.125 mM | 3.5 | 0.08 | 43 | pH 7.5, 30 °C | Open record: ATP-dependent dihydroxyacetone kinase, adenosine 5'-triphosphate |
| ATP-dependent dihydroxyacetone kinase | Citrobacter freundii | adenosine 5'-triphosphatebisubstrate kinetics; cosubstrate D,L-glyceraldehyde | — | 0.14 | — | pH 7.5, 30 °C | Open record: ATP-dependent dihydroxyacetone kinase, adenosine 5'-triphosphate |
| ATP-dependent dihydroxyacetone kinase | Citrobacter freundii | adenosine 5'-triphosphatecoupled assay: DhaP reduction with NADH and G3PDH | — | 0.1 | — | pH 7.5, 30 °C | Open record: ATP-dependent dihydroxyacetone kinase, adenosine 5'-triphosphate |
| ATP-dependent dihydroxyacetone kinase | Citrobacter freundii | adenosine 5'-triphosphateproduct-inhibition experiment (ADP) | — | 0.1 | — | pH 7.5, 30 °C | Open record: ATP-dependent dihydroxyacetone kinase, adenosine 5'-triphosphate |
| ATP-dependent dihydroxyacetone kinase | Citrobacter freundii | D,L-glyceraldehydebuffer A (100 mM Hepes pH 7.5, 5 mM MgCl2, 0.4 mM NADH) | 5.2 | 0.18 | 28 | pH 7.5, 30 °C | Open record: ATP-dependent dihydroxyacetone kinase, D,L-glyceraldehyde |
| ATP-dependent dihydroxyacetone kinase | Citrobacter freundii | D,L-glyceraldehydebisubstrate kinetics; Hepes pH 7.5 buffer A; apparent kcat | 4.7 | 0.14 | 33 | pH 7.5, 30 °C | Open record: ATP-dependent dihydroxyacetone kinase, D,L-glyceraldehyde |
| ATP-dependent dihydroxyacetone kinase | Citrobacter freundii | D,L-glyceraldehydebisubstrate kinetics; Hepes pH 7.5 buffer A; apparent kcat | 3.97 | 0.13 | 32 | pH 7.5, 30 °C | Open record: ATP-dependent dihydroxyacetone kinase, D,L-glyceraldehyde |
| ATP-dependent dihydroxyacetone kinase | Citrobacter freundii | D,L-glyceraldehydebisubstrate kinetics; Hepes pH 7.5 buffer A; apparent kcat | 3.2 | 0.09 | 35 | pH 7.5, 30 °C | Open record: ATP-dependent dihydroxyacetone kinase, D,L-glyceraldehyde |
| ATP-dependent dihydroxyacetone kinase | Citrobacter freundii | D,L-glyceraldehydebisubstrate kinetics; Hepes pH 7.5 buffer A; in the text | — | 0.12 | — | pH 7.5, 30 °C | Open record: ATP-dependent dihydroxyacetone kinase, D,L-glyceraldehyde |
| ATP-dependent dihydroxyacetone kinase | Citrobacter freundii | dihydroxyacetone | 17.5 | — | — | pH 7.5, 30 °C | Open record: ATP-dependent dihydroxyacetone kinase, dihydroxyacetone |
| ATP-dependent dihydroxyacetone kinase | Citrobacter freundii | hydroxyacetone | — | — | 0.75 | pH 7.5, 30 °C | Open record: ATP-dependent dihydroxyacetone kinase, hydroxyacetone |
| ATP-dependent dihydroxyacetone kinase | Citrobacter freundii | methylglyoxal | — | — | 0.33 | pH 7.5, 30 °C | Open record: ATP-dependent dihydroxyacetone kinase, methylglyoxal |
| PTS-dependent dihydroxyacetone kinase DhaK-DhaL complex | Escherichia coli | phospho-DhaM | — | 1.2 × 10⁻⁵ | — | pH 7.5, 30 °C | Open record: PTS-dependent dihydroxyacetone kinase DhaK-DhaL complex, phospho-DhaM |
| PTS-dependent dihydroxyacetone kinase DhaK/DhaL/DhaM | Escherichia coli | D,L-glyceraldehyde | 0.208 | 0.11 | 1.8 | pH 7.5, 30 °C | Open record: PTS-dependent dihydroxyacetone kinase DhaK/DhaL/DhaM, D,L-glyceraldehyde |
| PTS-dependent dihydroxyacetone kinase DhaK/DhaL/DhaM | Escherichia coli | dihydroxyacetone | 4.8 | — | — | pH 7.5, 30 °C | Open record: PTS-dependent dihydroxyacetone kinase DhaK/DhaL/DhaM, dihydroxyacetone |