Values CatLog read from this paper
Isotope and elemental effects indicate a rate-limiting methyl transfer as the initial step in the reaction catalyzed by Escherichia coli cyclopropane fatty acid synthase
PMID 15491158doi:10.1021/bi048692hOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 9 records (20 values) from this paper: cyclopropane fatty acid synthase (CFA synthase), catechol O-methyltransferase (COMT), AdoHcys nucleosidase (MTA/AdoHcys nucleosidase) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 1 kcat/Km value marked “calc.” from kcat and Km; no printed kcat/Km was read for that record.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| AdoHcys nucleosidase (MTA/AdoHcys nucleosidase) | Escherichia coli | Te-adenosyl-L-telluromethionine | 0.00187 | 0.128 | 0.0146 calc. | pH 7.5 | Open record: AdoHcys nucleosidase (MTA/AdoHcys nucleosidase), Te-adenosyl-L-telluromethionine |
| catechol O-methyltransferase (COMT) | — | S-adenosyl-L-methionine | — | 0.00492 | — | pH 7.5, 37 °C | Open record: catechol O-methyltransferase (COMT), S-adenosyl-L-methionine |
| catechol O-methyltransferase (COMT) | — | Se-adenosyl-L-selenomethionine | — | 0.00461 | — | pH 7.5, 37 °C | Open record: catechol O-methyltransferase (COMT), Se-adenosyl-L-selenomethionine |
| catechol O-methyltransferase (COMT) | — | Te-adenosyl-L-telluromethionine | — | 0.0875 | — | pH 7.5, 37 °C | Open record: catechol O-methyltransferase (COMT), Te-adenosyl-L-telluromethionine |
| cyclopropane fatty acid synthase (CFA synthase) | Escherichia coli | S-adenosyl-L-[methyl-d3]methionine | 0.227 | 0.0544 | 4.17 | pH 7.5, 37 °C | Open record: cyclopropane fatty acid synthase (CFA synthase), S-adenosyl-L-[methyl-d3]methionine |
| cyclopropane fatty acid synthase (CFA synthase) | Escherichia coli | S-adenosyl-L-methioninefit to Michaelis-Menten eq 2; 0.5 µM CFA synthase | 0.1218 | 0.0894 | 1.363 | pH 7.5, 37 °C | Open record: cyclopropane fatty acid synthase (CFA synthase), S-adenosyl-L-methionine |
| cyclopropane fatty acid synthase (CFA synthase) | Escherichia coli | S-adenosyl-L-methionineAdoMet or [methyl-d3]AdoMet varied 5-500 µM (Methods) | 0.197 | 0.0531 | 3.7 | pH 7.5, 37 °C | Open record: cyclopropane fatty acid synthase (CFA synthase), S-adenosyl-L-methionine |
| cyclopropane fatty acid synthase (CFA synthase) | Escherichia coli | Se-adenosyl-L-selenomethionine | 0.217 | 0.0564 | 3.83 | pH 7.5, 37 °C | Open record: cyclopropane fatty acid synthase (CFA synthase), Se-adenosyl-L-selenomethionine |
| cyclopropane fatty acid synthase (CFA synthase) | Escherichia coli | Te-adenosyl-L-telluromethionine | 0.0603 | 0.74 | 0.0815 | pH 7.5, 37 °C | Open record: cyclopropane fatty acid synthase (CFA synthase), Te-adenosyl-L-telluromethionine |