Values CatLog read from this paper
A thermostable variant of fructose bisphosphate aldolase constructed by directed evolution also shows increased stability in organic solvents
PMID 15531627doi:10.1093/protein/gzh081Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 9 records (18 values) from this paper: Class II fructose-1,6-bisphosphate aldolase (FBP-aldolase) from Edwardsiella ictaluri, Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 9 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| Class II fructose-1,6-bisphosphate aldolase (FBP-aldolase)4-43D6 I71K (K71/V210 mutant) | — | D-fructose 1,6-bisphosphate | 6 | 0.14 | 43 calc. | 30 °C | Open record: Class II fructose-1,6-bisphosphate aldolase (FBP-aldolase) 4-43D6 I71K (K71/V210 mutant), D-fructose 1,6-bisphosphate |
| Class II fructose-1,6-bisphosphate aldolase (FBP-aldolase)4-43D6 V210A (I71/A210 mutant) | — | D-fructose 1,6-bisphosphate | 12.5 | 0.1 | 120 calc. | 30 °C | Open record: Class II fructose-1,6-bisphosphate aldolase (FBP-aldolase) 4-43D6 V210A (I71/A210 mutant), D-fructose 1,6-bisphosphate |
| Class II fructose-1,6-bisphosphate aldolase (FBP-aldolase)variant 1-44F2 | — | D-fructose 1,6-bisphosphate | 14.5 | 0.15 | 97 calc. | 30 °C | Open record: Class II fructose-1,6-bisphosphate aldolase (FBP-aldolase) variant 1-44F2, D-fructose 1,6-bisphosphate |
| Class II fructose-1,6-bisphosphate aldolase (FBP-aldolase)variant 2-15B2 | — | D-fructose 1,6-bisphosphate | 22.5 | 0.1 | 220 calc. | 30 °C | Open record: Class II fructose-1,6-bisphosphate aldolase (FBP-aldolase) variant 2-15B2, D-fructose 1,6-bisphosphate |
| Class II fructose-1,6-bisphosphate aldolase (FBP-aldolase)variant 3-4C10 | — | D-fructose 1,6-bisphosphate | 10.83 | 0.12 | 90 calc. | 30 °C | Open record: Class II fructose-1,6-bisphosphate aldolase (FBP-aldolase) variant 3-4C10, D-fructose 1,6-bisphosphate |
| Class II fructose-1,6-bisphosphate aldolase (FBP-aldolase)variant 4-43D6 | — | D-fructose 1,6-bisphosphate | 13 | 0.18 | 72 calc. | 30 °C | Open record: Class II fructose-1,6-bisphosphate aldolase (FBP-aldolase) variant 4-43D6, D-fructose 1,6-bisphosphate |
| Class II fructose-1,6-bisphosphate aldolase (FBP-aldolase)variant 4-4C10 | — | D-fructose 1,6-bisphosphate | 3.5 | 0.12 | 29 calc. | 30 °C | Open record: Class II fructose-1,6-bisphosphate aldolase (FBP-aldolase) variant 4-4C10, D-fructose 1,6-bisphosphate |
| Class II fructose-1,6-bisphosphate aldolase (FBP-aldolase) | Edwardsiella ictaluri | D-fructose 1,6-bisphosphate | 13 | 0.15 | 87 calc. | 30 °C | Open record: Class II fructose-1,6-bisphosphate aldolase (FBP-aldolase), D-fructose 1,6-bisphosphate |
| Class II fructose-1,6-bisphosphate aldolase (FBP-aldolase) | Escherichia coli | D-fructose 1,6-bisphosphate | 14.17 | 0.14 | 100 calc. | 30 °C | Open record: Class II fructose-1,6-bisphosphate aldolase (FBP-aldolase), D-fructose 1,6-bisphosphate |