CatLogenzyme kinetics catalog

Values CatLog read from this paper

Catalytic mechanism of alpha-retaining glucosyl transfer by Corynebacterium callunae starch phosphorylase: the role of histidine-334 examined through kinetic characterization of site-directed…

PMID 15535798doi:10.1042/bj20041593Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 24 records (32 values) from this paper: starch phosphorylase (CcStP) from Corynebacterium callunae.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 8 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.4.1.1

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
starch phosphorylase (CcStP)Corynebacterium callunaealpha-D-glucose 1-phosphate520.8561 calc.Open
starch phosphorylase (CcStP)Corynebacterium callunaealpha-D-xylose 1-phosphate0.0026——pH 6, 30 °COpen
starch phosphorylase (CcStP)Corynebacterium callunaeglycogen—1.6—pH 7, 30 °COpen
starch phosphorylase (CcStP)Corynebacterium callunaemaltopentaose—3.8—pH 7, 30 °COpen
starch phosphorylase (CcStP)Corynebacterium callunaephosphate—6.8—pH 7, 30 °COpen
starch phosphorylase (CcStP)Corynebacterium callunaestarch740.23320 calc.pH 7, 30 °COpen
starch phosphorylase (CcStP)variant H334ACorynebacterium callunaealpha-D-glucose 1-phosphate491.827 calc.Open
starch phosphorylase (CcStP)variant H334ACorynebacterium callunaealpha-D-xylose 1-phosphate0.0028——pH 6, 30 °COpen
starch phosphorylase (CcStP)variant H334ACorynebacterium callunaeglycogen—1.6—pH 7, 30 °COpen
starch phosphorylase (CcStP)variant H334ACorynebacterium callunaemaltopentaose—3.5—pH 7, 30 °COpen
starch phosphorylase (CcStP)variant H334ACorynebacterium callunaephosphate—12—pH 7, 30 °COpen
starch phosphorylase (CcStP)variant H334ACorynebacterium callunaestarch800.25320 calc.pH 7, 30 °COpen
starch phosphorylase (CcStP)variant H334NCorynebacterium callunaealpha-D-glucose 1-phosphate1.271.30.98 calc.Open
starch phosphorylase (CcStP)variant H334NCorynebacterium callunaealpha-D-xylose 1-phosphate4.8 × 10⁻⁴——pH 6, 30 °COpen
starch phosphorylase (CcStP)variant H334NCorynebacterium callunaeglycogen—16—pH 7, 30 °COpen
starch phosphorylase (CcStP)variant H334NCorynebacterium callunaemaltopentaose—103—pH 7, 30 °COpen
starch phosphorylase (CcStP)variant H334NCorynebacterium callunaephosphate—13—pH 7, 30 °COpen
starch phosphorylase (CcStP)variant H334NCorynebacterium callunaestarch1.511.5 calc.pH 7, 30 °COpen
starch phosphorylase (CcStP)variant H334QCorynebacterium callunaealpha-D-glucose 1-phosphate0.681.10.62 calc.Open
starch phosphorylase (CcStP)variant H334QCorynebacterium callunaealpha-D-xylose 1-phosphate0.0066——pH 6, 30 °COpen
starch phosphorylase (CcStP)variant H334QCorynebacterium callunaeglycogen—1.7—pH 7, 30 °COpen
starch phosphorylase (CcStP)variant H334QCorynebacterium callunaemaltopentaose—4.1—pH 7, 30 °COpen
starch phosphorylase (CcStP)variant H334QCorynebacterium callunaephosphate—12—pH 7, 30 °COpen
starch phosphorylase (CcStP)variant H334QCorynebacterium callunaestarch0.520.321.6 calc.pH 7, 30 °COpen