Values CatLog read from this paper

Site-directed mutagenesis of the active site region in the quinate/shikimate 5-dehydrogenase YdiB of Escherichia coli

PMID 15596430doi:10.1074/jbc.m412028200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 30 records (90 values) from this paper: quinate/shikimate 5-dehydrogenase YdiB from Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.282

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
quinate/shikimate 5-dehydrogenase YdiB	Escherichia coli	NAD+quinate fixed (20 µL of 40 mM quinate added per 210 µL well)	0.142	0.0184	7.717	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB, NAD+
quinate/shikimate 5-dehydrogenase YdiB	Escherichia coli	NAD+cosubstrate shikimate	0.105	0.0122	8.607	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB, NAD+
quinate/shikimate 5-dehydrogenase YdiB	Escherichia coli	quinate	0.113	0.0091	12.42	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB, quinate
quinate/shikimate 5-dehydrogenase YdiB	Escherichia coli	shikimate	0.091	0.0029	31.38	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB, shikimate
quinate/shikimate 5-dehydrogenase YdiBvariant D107A	Escherichia coli	NAD+	0.022	0.0068	3.235	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant D107A, NAD+
quinate/shikimate 5-dehydrogenase YdiBvariant D107A	Escherichia coli	shikimate	0.132	40.07	0.00329	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant D107A, shikimate
quinate/shikimate 5-dehydrogenase YdiBvariant K71G	Escherichia coli	NAD+quinate fixed (20 µL of 40 mM quinate added per 210 µL well)	0.004	0.002	2	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant K71G, NAD+
quinate/shikimate 5-dehydrogenase YdiBvariant K71G	Escherichia coli	NAD+cosubstrate shikimate	0.012	4 × 10⁻⁴	30	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant K71G, NAD+
quinate/shikimate 5-dehydrogenase YdiBvariant K71G	Escherichia coli	quinate	0.012	32.68	3.7 × 10⁻⁴	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant K71G, quinate
quinate/shikimate 5-dehydrogenase YdiBvariant K71G	Escherichia coli	shikimate	0.011	0.513	0.0214	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant K71G, shikimate
quinate/shikimate 5-dehydrogenase YdiBvariant Q262A	Escherichia coli	NAD+quinate fixed (20 µL of 40 mM quinate added per 210 µL well)	0.043	0.0142	3.028	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant Q262A, NAD+
quinate/shikimate 5-dehydrogenase YdiBvariant Q262A	Escherichia coli	NAD+cosubstrate shikimate	0.022	0.0107	2.056	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant Q262A, NAD+
quinate/shikimate 5-dehydrogenase YdiBvariant Q262A	Escherichia coli	quinate	0.05	0.0136	3.677	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant Q262A, quinate
quinate/shikimate 5-dehydrogenase YdiBvariant Q262A	Escherichia coli	shikimate	0.034	0.0028	12.14	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant Q262A, shikimate
quinate/shikimate 5-dehydrogenase YdiBvariant S22A	Escherichia coli	NAD+quinate fixed (20 µL of 40 mM quinate added per 210 µL well)	0.082	0.0168	4.881	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant S22A, NAD+
quinate/shikimate 5-dehydrogenase YdiBvariant S22A	Escherichia coli	NAD+cosubstrate shikimate	0.153	0.018	8.5	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant S22A, NAD+
quinate/shikimate 5-dehydrogenase YdiBvariant S22A	Escherichia coli	quinate	0.068	0.0057	11.93	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant S22A, quinate
quinate/shikimate 5-dehydrogenase YdiBvariant S22A	Escherichia coli	shikimate	0.109	0.0032	34.06	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant S22A, shikimate
quinate/shikimate 5-dehydrogenase YdiBvariant S67A	Escherichia coli	NAD+quinate fixed (20 µL of 40 mM quinate added per 210 µL well)	0.023	0.0121	1.901	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant S67A, NAD+
quinate/shikimate 5-dehydrogenase YdiBvariant S67A	Escherichia coli	NAD+cosubstrate shikimate	0.178	0.0126	14.13	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant S67A, NAD+
quinate/shikimate 5-dehydrogenase YdiBvariant S67A	Escherichia coli	quinate	0.024	0.0157	1.529	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant S67A, quinate
quinate/shikimate 5-dehydrogenase YdiBvariant S67A	Escherichia coli	shikimate	0.148	0.0017	87.06	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant S67A, shikimate
quinate/shikimate 5-dehydrogenase YdiBvariant T106A	Escherichia coli	NAD+quinate fixed (20 µL of 40 mM quinate added per 210 µL well)	0.122	0.0578	2.111	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant T106A, NAD+
quinate/shikimate 5-dehydrogenase YdiBvariant T106A	Escherichia coli	NAD+cosubstrate shikimate	0.108	0.0158	6.835	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant T106A, NAD+
quinate/shikimate 5-dehydrogenase YdiBvariant T106A	Escherichia coli	quinate	0.233	20.52	0.0114	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant T106A, quinate
quinate/shikimate 5-dehydrogenase YdiBvariant T106A	Escherichia coli	shikimate	0.054	0.199	0.271	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant T106A, shikimate
quinate/shikimate 5-dehydrogenase YdiBvariant Y39F	Escherichia coli	NAD+quinate fixed (20 µL of 40 mM quinate added per 210 µL well)	0.09	0.0125	7.2	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant Y39F, NAD+
quinate/shikimate 5-dehydrogenase YdiBvariant Y39F	Escherichia coli	NAD+cosubstrate shikimate	0.24	0.0158	15.19	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant Y39F, NAD+
quinate/shikimate 5-dehydrogenase YdiBvariant Y39F	Escherichia coli	quinate	0.06	0.0054	11.11	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant Y39F, quinate
quinate/shikimate 5-dehydrogenase YdiBvariant Y39F	Escherichia coli	shikimate	0.187	0.0032	58.44	pH 9, 20 °C	Open record: quinate/shikimate 5-dehydrogenase YdiB variant Y39F, shikimate