CatLogenzyme kinetics catalog

Values CatLog read from this paper

Complete reversal of coenzyme specificity of xylitol dehydrogenase and increase of thermostability by the introduction of structural zinc

PMID 15623532doi:10.1074/jbc.m409443200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 49 records (109 values) from this paper: xylitol dehydrogenase (PsXDH) from Pichia stipitis.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 1.1.1.9

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
xylitol dehydrogenase (PsXDH)Pichia stipitisNAD+17.50.38146pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)Pichia stipitisNADP+1.81700.01083pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)Pichia stipitisxylitol—21.7—pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207APichia stipitisNAD+5.170.40312.75pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207APichia stipitisNADP+5.671200.0467pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207APichia stipitisxylitol—22.2—pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207A/F209SPichia stipitisNAD+3.330.16220.8pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207A/F209SPichia stipitisNADP+89.968.07pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207A/F209SPichia stipitisxylitolcosubstrate NAD+—45.2—pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207A/F209SPichia stipitisxylitolcosubstrate NADP+—59.1—pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207A/I208RPichia stipitisNAD+10.330.56818.3pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207A/I208RPichia stipitisNADP+2811.32.48pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207A/I208RPichia stipitisxylitol—24.2—pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207A/I208R/F209SPichia stipitisNAD+41.33.02pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207A/I208R/F209SPichia stipitisNADP+41.70.89746.5pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207A/I208R/F209SPichia stipitisxylitolcosubstrate NAD+—55.7—pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207A/I208R/F209SPichia stipitisxylitolcosubstrate NADP+—31.1—pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207A/I208R/F209S/N211RPichia stipitisNAD+23.817.31.4pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207A/I208R/F209S/N211RPichia stipitisNADP+641.3846.5pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207A/I208R/F209S/N211RPichia stipitisxylitol—72.6—pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207A/I208R/F209TPichia stipitisNAD+5.170.26519.5pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207A/I208R/F209TPichia stipitisNADP+32.80.63851.5pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207A/I208R/F209TPichia stipitisxylitolcosubstrate NAD+—97.8—pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207A/I208R/F209TPichia stipitisxylitolcosubstrate NADP+—46.3—pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207A/I208R/F209YPichia stipitisNAD+1.0170.1526.7pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207A/I208R/F209YPichia stipitisNADP+6.170.7318.53pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207A/I208R/F209YPichia stipitisxylitolcosubstrate NAD+—50.1—pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant D207A/I208R/F209YPichia stipitisxylitolcosubstrate NADP+—58.6—pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant F209SPichia stipitisNAD+20.30.84824pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant F209SPichia stipitisNADP+9.8328.90.34pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant F209SPichia stipitisxylitol—34.1—pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant I208RPichia stipitisNAD+23.80.49847.5pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant I208RPichia stipitisNADP+1021.10.477pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant I208RPichia stipitisxylitol—29.5—pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant I208R/F209SPichia stipitisNAD+1.0050.6651.5pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant I208R/F209SPichia stipitisNADP+0.1459.190.0158pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant I208R/F209SPichia stipitisxylitol—45.4—pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant N211RPichia stipitisNAD+22.80.53842.5pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant N211RPichia stipitisNADP+4.6756.50.082pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant N211RPichia stipitisxylitol—27.4—pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant S96C/S99C/Y102CPichia stipitisNAD+30.30.73941pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant S96C/S99C/Y102CPichia stipitisNADP+0.919.560.095pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant S96C/S99C/Y102CPichia stipitisxylitol—30.3—pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant S96C/S99C/Y102C/D207A/I208R/F209SPichia stipitisNAD+29.523.51.255pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant S96C/S99C/Y102C/D207A/I208R/F209SPichia stipitisNADP+2101.18178pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant S96C/S99C/Y102C/D207A/I208R/F209SPichia stipitisxylitol—111—pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant S96C/S99C/Y102C/D207A/I208R/F209S/N211RPichia stipitisNAD+13.27.61.73pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant S96C/S99C/Y102C/D207A/I208R/F209S/N211RPichia stipitisNADP+1831.04175pH 9, 35 °COpen
xylitol dehydrogenase (PsXDH)variant S96C/S99C/Y102C/D207A/I208R/F209S/N211RPichia stipitisxylitol—119—pH 9, 35 °COpen