Values CatLog read from this paper

Structural and kinetic analyses of arginine residues in the active site of the acetate kinase from Methanosarcina thermophila

PMID 15647264doi:10.1074/jbc.m412118200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 35 records (52 values) from this paper: acetate kinase from Methanosarcina thermophila.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 17 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 2.7.2.1

Records read from this paper
Enzyme	Organism	Substrate	k_cat (s⁻¹)	K_m (mM)	k_cat/K_m (mM⁻¹ s⁻¹)	Conditions	Open record
acetate kinase	Methanosarcina thermophila	acetate	913	2.7	340 calc.	pH 7	Open record: acetate kinase, acetate
acetate kinase	Methanosarcina thermophila	acetate200 mM guanidine hydrochloride	428	24	18 calc.	pH 7	Open record: acetate kinase, acetate
acetate kinase	Methanosarcina thermophila	acetyl phosphate	1.26 × 10³	0.47	2.7 × 10³ calc.	pH 7.4	Open record: acetate kinase, acetyl phosphate
acetate kinase	Methanosarcina thermophila	ADP	—	0.098	—	pH 7.4	Open record: acetate kinase, ADP
acetate kinase	Methanosarcina thermophila	ATP60 mM Hepes	—	0.08	—	pH 7	Open record: acetate kinase, ATP
acetate kinase	Methanosarcina thermophila	ATP60 mM Hepes; 200 mM guanidine hydrochloride	—	0.068	—	pH 7	Open record: acetate kinase, ATP
acetate kinase	Methanosarcina thermophila	ATP60 mM HEPES; 1 μg/ml wild-type enzyme	—	0.065	—	pH 7	Open record: acetate kinase, ATP
acetate kinasevariant R241A	Methanosarcina thermophila	acetate	0.68	710	9.6 × 10⁻⁴ calc.	pH 7	Open record: acetate kinase variant R241A, acetate
acetate kinasevariant R241A	Methanosarcina thermophila	acetate200 mM guanidine hydrochloride	0.53	30	0.018 calc.	pH 7	Open record: acetate kinase variant R241A, acetate
acetate kinasevariant R241A	Methanosarcina thermophila	acetyl phosphate	4.5	0.84	5.4 calc.	pH 7.4	Open record: acetate kinase variant R241A, acetyl phosphate
acetate kinasevariant R241A	Methanosarcina thermophila	ADP	—	0.21	—	pH 7.4	Open record: acetate kinase variant R241A, ADP
acetate kinasevariant R241A	Methanosarcina thermophila	ATP	—	0.297	—	pH 7	Open record: acetate kinase variant R241A, ATP
acetate kinasevariant R241A	Methanosarcina thermophila	ATP200 mM guanidine hydrochloride	—	0.264	—	pH 7	Open record: acetate kinase variant R241A, ATP
acetate kinasevariant R241K	Methanosarcina thermophila	acetate	1.3	77	0.017 calc.	pH 7	Open record: acetate kinase variant R241K, acetate
acetate kinasevariant R241K	Methanosarcina thermophila	acetyl phosphate	4.5	0.92	4.9 calc.	pH 7.4	Open record: acetate kinase variant R241K, acetyl phosphate
acetate kinasevariant R241K	Methanosarcina thermophila	ADP	—	0.578	—	pH 7.4	Open record: acetate kinase variant R241K, ADP
acetate kinasevariant R241K	Methanosarcina thermophila	ATP	—	11.4	—	pH 7	Open record: acetate kinase variant R241K, ATP
acetate kinasevariant R241L	Methanosarcina thermophila	acetate	1.38	270	0.0051 calc.	pH 7	Open record: acetate kinase variant R241L, acetate
acetate kinasevariant R241L	Methanosarcina thermophila	acetyl phosphate	0.42	1.64	0.26 calc.	pH 7.4	Open record: acetate kinase variant R241L, acetyl phosphate
acetate kinasevariant R241L	Methanosarcina thermophila	ADP	—	1.26	—	pH 7.4	Open record: acetate kinase variant R241L, ADP
acetate kinasevariant R241L	Methanosarcina thermophila	ATP	—	17	—	pH 7	Open record: acetate kinase variant R241L, ATP
acetate kinasevariant R91A	Methanosarcina thermophila	acetate	0.11	250	4.4 × 10⁻⁴ calc.	pH 7	Open record: acetate kinase variant R91A, acetate
acetate kinasevariant R91A	Methanosarcina thermophila	acetate200 mM guanidine hydrochloride	1.7	83	0.020 calc.	pH 7	Open record: acetate kinase variant R91A, acetate
acetate kinasevariant R91A	Methanosarcina thermophila	acetyl phosphate	3.5	1.36	2.6 calc.	pH 7.4	Open record: acetate kinase variant R91A, acetyl phosphate
acetate kinasevariant R91A	Methanosarcina thermophila	ADP	—	0.063	—	pH 7.4	Open record: acetate kinase variant R91A, ADP
acetate kinasevariant R91A	Methanosarcina thermophila	ATP	—	0.016	—	pH 7	Open record: acetate kinase variant R91A, ATP
acetate kinasevariant R91A	Methanosarcina thermophila	ATP200 mM guanidine hydrochloride	—	0.068	—	pH 7	Open record: acetate kinase variant R91A, ATP
acetate kinasevariant R91K	Methanosarcina thermophila	acetate	3.7	70	0.053 calc.	pH 7	Open record: acetate kinase variant R91K, acetate
acetate kinasevariant R91K	Methanosarcina thermophila	acetyl phosphate	23	0.61	38 calc.	pH 7.4	Open record: acetate kinase variant R91K, acetyl phosphate
acetate kinasevariant R91K	Methanosarcina thermophila	ADP	—	0.168	—	pH 7.4	Open record: acetate kinase variant R91K, ADP
acetate kinasevariant R91K	Methanosarcina thermophila	ATP	—	0.076	—	pH 7	Open record: acetate kinase variant R91K, ATP
acetate kinasevariant R91L	Methanosarcina thermophila	acetate	0.22	420	5.2 × 10⁻⁴ calc.	pH 7	Open record: acetate kinase variant R91L, acetate
acetate kinasevariant R91L	Methanosarcina thermophila	acetyl phosphate	7.7	2.3	3.3 calc.	pH 7.4	Open record: acetate kinase variant R91L, acetyl phosphate
acetate kinasevariant R91L	Methanosarcina thermophila	ADP	—	0.063	—	pH 7.4	Open record: acetate kinase variant R91L, ADP
acetate kinasevariant R91L	Methanosarcina thermophila	ATP	—	0.145	—	pH 7	Open record: acetate kinase variant R91L, ATP