Values CatLog read from this paper
Evidence for two different mechanisms triggering the change in quaternary structure of the allosteric enzyme, glucosamine-6-phosphate deaminase
PMID 15667206doi:10.1021/bi048514oOpen in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 14 records (21 values) from this paper: glucosamine-6-phosphate deaminase from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 7 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| glucosamine-6-phosphate deaminase | Escherichia coli | glucosamine 6-phosphatein the presence of 1 mM (saturating) GlcNAc6P | 158 | 0.55 | 290 calc. | pH 7.5, 30 °C | Open record: glucosamine-6-phosphate deaminase, glucosamine 6-phosphate |
| glucosamine-6-phosphate deaminase | Escherichia coli | glucosamine 6-phosphatein the absence of GlcNAc6P (allosteric activator) | 157 | — | — | pH 7.5, 30 °C | Open record: glucosamine-6-phosphate deaminase, glucosamine 6-phosphate |
| glucosamine-6-phosphate deaminasevariant W15Y | Escherichia coli | glucosamine 6-phosphatein the presence of 1 mM (saturating) GlcNAc6P | 134 | 0.62 | 220 calc. | pH 7.5, 30 °C | Open record: glucosamine-6-phosphate deaminase variant W15Y, glucosamine 6-phosphate |
| glucosamine-6-phosphate deaminasevariant W15Y | Escherichia coli | glucosamine 6-phosphatein the absence of GlcNAc6P (allosteric activator) | 130 | — | — | pH 7.5, 30 °C | Open record: glucosamine-6-phosphate deaminase variant W15Y, glucosamine 6-phosphate |
| glucosamine-6-phosphate deaminasevariant W15Y/F174W/W224Y | Escherichia coli | glucosamine 6-phosphatein the presence of 1 mM (saturating) GlcNAc6P | 80 | 0.49 | 160 calc. | pH 7.5, 30 °C | Open record: glucosamine-6-phosphate deaminase variant W15Y/F174W/W224Y, glucosamine 6-phosphate |
| glucosamine-6-phosphate deaminasevariant W15Y/F174W/W224Y | Escherichia coli | glucosamine 6-phosphatein the absence of GlcNAc6P (allosteric activator) | 74 | — | — | pH 7.5, 30 °C | Open record: glucosamine-6-phosphate deaminase variant W15Y/F174W/W224Y, glucosamine 6-phosphate |
| glucosamine-6-phosphate deaminasevariant W15Y/W224Y | Escherichia coli | glucosamine 6-phosphatein the presence of 1 mM (saturating) GlcNAc6P | 144 | 0.65 | 220 calc. | pH 7.5, 30 °C | Open record: glucosamine-6-phosphate deaminase variant W15Y/W224Y, glucosamine 6-phosphate |
| glucosamine-6-phosphate deaminasevariant W15Y/W224Y | Escherichia coli | glucosamine 6-phosphatein the absence of GlcNAc6P (allosteric activator) | 149 | — | — | pH 7.5, 30 °C | Open record: glucosamine-6-phosphate deaminase variant W15Y/W224Y, glucosamine 6-phosphate |
| glucosamine-6-phosphate deaminasevariant W15Y/W224Y/K234W | Escherichia coli | glucosamine 6-phosphatein the presence of 1 mM (saturating) GlcNAc6P | 159 | 0.49 | 320 calc. | pH 7.5, 30 °C | Open record: glucosamine-6-phosphate deaminase variant W15Y/W224Y/K234W, glucosamine 6-phosphate |
| glucosamine-6-phosphate deaminasevariant W15Y/W224Y/K234W | Escherichia coli | glucosamine 6-phosphatein the absence of GlcNAc6P (allosteric activator) | 109 | — | — | pH 7.5, 30 °C | Open record: glucosamine-6-phosphate deaminase variant W15Y/W224Y/K234W, glucosamine 6-phosphate |
| glucosamine-6-phosphate deaminasevariant W15Y/W224Y/Y254W | Escherichia coli | glucosamine 6-phosphatein the presence of 1 mM (saturating) GlcNAc6P | 26.7 | 0.26 | 100 calc. | pH 7.5, 30 °C | Open record: glucosamine-6-phosphate deaminase variant W15Y/W224Y/Y254W, glucosamine 6-phosphate |
| glucosamine-6-phosphate deaminasevariant W15Y/W224Y/Y254W | Escherichia coli | glucosamine 6-phosphatein the absence of GlcNAc6P (allosteric activator) | 25.7 | — | — | pH 7.5, 30 °C | Open record: glucosamine-6-phosphate deaminase variant W15Y/W224Y/Y254W, glucosamine 6-phosphate |
| glucosamine-6-phosphate deaminasevariant W224Y | Escherichia coli | glucosamine 6-phosphatein the presence of 1 mM (saturating) GlcNAc6P | 138 | 0.5 | 280 calc. | pH 7.5, 30 °C | Open record: glucosamine-6-phosphate deaminase variant W224Y, glucosamine 6-phosphate |
| glucosamine-6-phosphate deaminasevariant W224Y | Escherichia coli | glucosamine 6-phosphatein the absence of GlcNAc6P (allosteric activator) | 135 | — | — | pH 7.5, 30 °C | Open record: glucosamine-6-phosphate deaminase variant W224Y, glucosamine 6-phosphate |