CatLogenzyme kinetics catalog

Values CatLog read from this paper

Mutation of arginine 228 to lysine enhances the glucosyltransferase activity of bovine beta-1,4-galactosyltransferase I

PMID 15736931doi:10.1021/bi0479454Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 8 records (20 values) from this paper: β-1,4-galactosyltransferase I (β4Gal-T1) from Bos taurus.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.4.1.38

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
β-1,4-galactosyltransferase I (β4Gal-T1)variant R228KBos taurusN-acetylglucosamineGlc-T reaction (Glc transfer from UDP-Glc to GlcNAc)0.1790.018830 °COpen
β-1,4-galactosyltransferase I (β4Gal-T1)variant R228KBos taurusN-acetylglucosamineGlc-T reaction (Glc transfer from UDP-Glc to GlcNAc)0.961.30.7330 °COpen
β-1,4-galactosyltransferase I (β4Gal-T1)variant R228KBos taurusN-acetylglucosamineGal-T reaction (Gal transfer from UDP-Gal to GlcNAc)0.52110.0430 °COpen
β-1,4-galactosyltransferase I (β4Gal-T1)variant R228KBos taurusN-acetylglucosamineGal-T reaction (Gal transfer from UDP-Gal to GlcNAc)0.330.70.4730 °COpen
β-1,4-galactosyltransferase I (β4Gal-T1)variant R228KBos taurusuridine 5'-diphosphogalactosewithout alpha-lactalbumin (-LA)—0.087530 °COpen
β-1,4-galactosyltransferase I (β4Gal-T1)variant R228KBos taurusuridine 5'-diphosphogalactosewith alpha-lactalbumin (+LA, 7 µg LA)—0.01053030 °COpen
β-1,4-galactosyltransferase I (β4Gal-T1)variant R228KBos taurusuridine 5'-diphosphoglucosewithout alpha-lactalbumin (-LA)—0.0911.830 °COpen
β-1,4-galactosyltransferase I (β4Gal-T1)variant R228KBos taurusuridine 5'-diphosphoglucosewith alpha-lactalbumin (+LA, 7 µg LA, saturating)—0.0442030 °COpen