Values CatLog read from this paper
Probing the role of Asp-120(81) of metallo-beta-lactamase (IMP-1) by site-directed mutagenesis, kinetic studies, and X-ray crystallography
PMID 15788415doi:10.1074/jbc.m414314200Open in Browse
The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.
CatLog read 17 records (51 values) from this paper: metallo-beta-lactamase IMP-1 from Serratia marcescens.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| metallo-beta-lactamase IMP-1 | Serratia marcescens | Benzyl penicillin | 650 | 0.8 | 830 | pH 7.4, 30 °C | Open record: metallo-beta-lactamase IMP-1, Benzyl penicillin |
| metallo-beta-lactamase IMP-1 | Serratia marcescens | Cephaloridine | 90 | 0.008 | 1.13 × 10⁴ | pH 7.4, 30 °C | Open record: metallo-beta-lactamase IMP-1, Cephaloridine |
| metallo-beta-lactamase IMP-1 | Serratia marcescens | Imipenem | 123.3 | 0.045 | 1.55 × 10³ | pH 7.4, 30 °C | Open record: metallo-beta-lactamase IMP-1, Imipenem |
| metallo-beta-lactamase IMP-1 | Serratia marcescens | Nitrocefin | 470 | 0.02 | 2.3 × 10⁴ | pH 7.4, 30 °C | Open record: metallo-beta-lactamase IMP-1, Nitrocefin |
| metallo-beta-lactamase IMP-1variant D81A | Serratia marcescens | Benzyl penicillin | 0.167 | 8 | 0.05 | pH 7.4, 30 °C | Open record: metallo-beta-lactamase IMP-1 variant D81A, Benzyl penicillin |
| metallo-beta-lactamase IMP-1variant D81A | Serratia marcescens | Cephaloridine(50 mM, pH 7.4, 0.5 M NaCl) | 0.0035 | 0.15 | 0.027 | pH 7.4, 30 °C | Open record: metallo-beta-lactamase IMP-1 variant D81A, Cephaloridine |
| metallo-beta-lactamase IMP-1variant D81A | Serratia marcescens | Cephaloridine(50 mM, pH 7.4, 0.5 M NaCl) with 1 mM Zn(NO3)2 added | 0.022 | 0.2 | 0.108 | pH 7.4, 30 °C | Open record: metallo-beta-lactamase IMP-1 variant D81A, Cephaloridine |
| metallo-beta-lactamase IMP-1variant D81A | Serratia marcescens | Imipenem | 3.2 | 3.1 | 1.2 | pH 7.4, 30 °C | Open record: metallo-beta-lactamase IMP-1 variant D81A, Imipenem |
| metallo-beta-lactamase IMP-1variant D81A | Serratia marcescens | Nitrocefin(50 mM, pH 7.4, 0.5 M NaCl) | 7.5 | 0.027 | 280 | pH 7.4, 30 °C | Open record: metallo-beta-lactamase IMP-1 variant D81A, Nitrocefin |
| metallo-beta-lactamase IMP-1variant D81A | Serratia marcescens | Nitrocefin(50 mM, pH 7.4, 0.5 M NaCl) with 1 mM Zn(NO3)2 added | 4.2 | 0.05 | 87 | pH 7.4, 30 °C | Open record: metallo-beta-lactamase IMP-1 variant D81A, Nitrocefin |
| metallo-beta-lactamase IMP-1variant D81E | Serratia marcescens | Benzyl penicillin | 0.53 | 2.6 | 0.2 | pH 7.4, 30 °C | Open record: metallo-beta-lactamase IMP-1 variant D81E, Benzyl penicillin |
| metallo-beta-lactamase IMP-1variant D81E | Serratia marcescens | Cephaloridine(50 mM, pH 7.4, 0.5 M NaCl) | 0.233 | 0.27 | 0.83 | pH 7.4, 30 °C | Open record: metallo-beta-lactamase IMP-1 variant D81E, Cephaloridine |
| metallo-beta-lactamase IMP-1variant D81E | Serratia marcescens | Cephaloridine(50 mM, pH 7.4, 0.5 M NaCl) with 1 mM Zn(NO3)2 added | 0.222 | 0.2 | 5 | pH 7.4, 30 °C | Open record: metallo-beta-lactamase IMP-1 variant D81E, Cephaloridine |
| metallo-beta-lactamase IMP-1variant D81E | Serratia marcescens | Imipenem(50 mM, pH 7.4, 0.5 M NaCl) | 2.03 | 0.64 | 3.3 | pH 7.4, 30 °C | Open record: metallo-beta-lactamase IMP-1 variant D81E, Imipenem |
| metallo-beta-lactamase IMP-1variant D81E | Serratia marcescens | Imipenem(50 mM, pH 7.4, 0.5 M NaCl) with 1 mM Zn(NO3)2 added | 3.7 | 0.75 | 5 | pH 7.4, 30 °C | Open record: metallo-beta-lactamase IMP-1 variant D81E, Imipenem |
| metallo-beta-lactamase IMP-1variant D81E | Serratia marcescens | Nitrocefin(50 mM, pH 7.4, 0.5 M NaCl) | 0.27 | 0.0013 | 200 | pH 7.4, 30 °C | Open record: metallo-beta-lactamase IMP-1 variant D81E, Nitrocefin |
| metallo-beta-lactamase IMP-1variant D81E | Serratia marcescens | Nitrocefin(50 mM, pH 7.4, 0.5 M NaCl) with 1 mM Zn(NO3)2 added | 0.55 | 0.0012 | 470 | pH 7.4, 30 °C | Open record: metallo-beta-lactamase IMP-1 variant D81E, Nitrocefin |