CatLogenzyme kinetics catalog

Values CatLog read from this paper

Molecular conversion of NAD kinase to NADH kinase through single amino acid residue substitution

PMID 15855156doi:10.1074/jbc.m502518200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 22 records (22 values) from this paper: Mfnk (poly(P)/ATP-NAD kinase), YfjB (ATP-NAD kinase) from Micrococcus flavus, Escherichia coli.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.

EC 2.7.1.23

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
Mfnk (poly(P)/ATP-NAD kinase)Micrococcus flavusadenosine 5'-triphosphateNAD kinase activity; cosubstrate NAD+—0.23—pH 7, 37 °COpen
Mfnk (poly(P)/ATP-NAD kinase)Micrococcus flavusadenosine 5'-triphosphateNADH kinase activity; cosubstrate NADH—0.32—pH 7, 37 °COpen
Mfnk (poly(P)/ATP-NAD kinase)Micrococcus flavusnicotinamide adenine dinucleotide (oxidized)phosphoryl donor ATP; cosubstrate ATP—0.53—pH 7, 37 °COpen
Mfnk (poly(P)/ATP-NAD kinase)Micrococcus flavusnicotinamide adenine dinucleotide (oxidized)phosphoryl donor Poly(P)_4; cosubstrate Poly(P)_4—0.26—pH 7, 37 °COpen
Mfnk (poly(P)/ATP-NAD kinase)Micrococcus flavusnicotinamide adenine dinucleotide (reduced)phosphoryl donor ATP; cosubstrate ATP—0.45—pH 7, 37 °COpen
Mfnk (poly(P)/ATP-NAD kinase)Micrococcus flavusnicotinamide adenine dinucleotide (reduced)phosphoryl donor Poly(P)_4; cosubstrate Poly(P)_4—0.35—pH 7, 37 °COpen
Mfnk (poly(P)/ATP-NAD kinase)Micrococcus flavustetrapolyphosphateNAD kinase activity; cosubstrate NAD+—0.33—pH 7, 37 °COpen
Mfnk (poly(P)/ATP-NAD kinase)Micrococcus flavustetrapolyphosphateNADH kinase activity; cosubstrate NADH—0.58—pH 7, 37 °COpen
Mfnk (poly(P)/ATP-NAD kinase)variant G183RMicrococcus flavusadenosine 5'-triphosphateNAD kinase activity; cosubstrate NAD+—0.16—pH 7, 37 °COpen
Mfnk (poly(P)/ATP-NAD kinase)variant G183RMicrococcus flavusadenosine 5'-triphosphateNADH kinase activity; cosubstrate NADH—0.47—pH 7, 37 °COpen
Mfnk (poly(P)/ATP-NAD kinase)variant G183RMicrococcus flavusnicotinamide adenine dinucleotide (oxidized)phosphoryl donor ATP; cosubstrate ATP—1.43—pH 7, 37 °COpen
Mfnk (poly(P)/ATP-NAD kinase)variant G183RMicrococcus flavusnicotinamide adenine dinucleotide (oxidized)phosphoryl donor Poly(P)_4; cosubstrate Poly(P)_4—1.86—pH 7, 37 °COpen
Mfnk (poly(P)/ATP-NAD kinase)variant G183RMicrococcus flavusnicotinamide adenine dinucleotide (reduced)phosphoryl donor ATP; cosubstrate ATP—0.74—pH 7, 37 °COpen
Mfnk (poly(P)/ATP-NAD kinase)variant G183RMicrococcus flavusnicotinamide adenine dinucleotide (reduced)phosphoryl donor Poly(P)_4; cosubstrate Poly(P)_4—0.45—pH 7, 37 °COpen
Mfnk (poly(P)/ATP-NAD kinase)variant G183RMicrococcus flavustetrapolyphosphateNAD kinase activity; cosubstrate NAD+—0.21—pH 7, 37 °COpen
Mfnk (poly(P)/ATP-NAD kinase)variant G183RMicrococcus flavustetrapolyphosphateNADH kinase activity; cosubstrate NADH—0.64—pH 7, 37 °COpen
YfjB (ATP-NAD kinase)Escherichia coliadenosine 5'-triphosphate—0.23—pH 7, 37 °COpen
YfjB (ATP-NAD kinase)Escherichia colinicotinamide adenine dinucleotide (oxidized)—1.85—pH 7, 37 °COpen
YfjB (ATP-NAD kinase)variant R175GEscherichia coliadenosine 5'-triphosphateNAD kinase activity; cosubstrate NAD+—0.2—pH 7, 37 °COpen
YfjB (ATP-NAD kinase)variant R175GEscherichia coliadenosine 5'-triphosphateNADH kinase activity; cosubstrate NADH—0.25—pH 7, 37 °COpen
YfjB (ATP-NAD kinase)variant R175GEscherichia colinicotinamide adenine dinucleotide (oxidized)—2—pH 7, 37 °COpen
YfjB (ATP-NAD kinase)variant R175GEscherichia colinicotinamide adenine dinucleotide (reduced)—2.13—pH 7, 37 °COpen