CatLogenzyme kinetics catalog

Values CatLog read from this paper

Dramatic broadening of the substrate profile of the Aeromonas hydrophila CphA metallo-beta-lactamase by site-directed mutagenesis

PMID 15863831doi:10.1074/jbc.m414052200Open in Browse

The paper itself is not on this site: read it at the publisher. This page lists only the values CatLog read from it.

CatLog read 42 records (111 values) from this paper: CphA metallo-beta-lactamase from Aeromonas hydrophila.

Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks. CatLog calculated the 15 kcat/Km values marked “calc.” from kcat and Km; no printed kcat/Km was read for those records.

EC 3.5.2.6

EnzymeOrganismSubstratekcat (s⁻¹)Km (mM)kcat/Km (mM⁻¹ s⁻¹)Conditions
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaAmpicillin0.80.81pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaBenzylpenicillinno added zinc (free Zn2+ < 0.4 µM); mono-zinc enzyme3.30.1522pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaBenzylpenicillinsaturating zinc concentration200.15130pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaBenzylpenicillin100 µM added zinc200.15130 calc.pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaCefotaximeno added zinc (free Zn2+ < 0.4 µM); mono-zinc enzyme0.30.056pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaCefotaximesaturating zinc concentration40.0580pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaCefotaxime100 µM added zinc40.0580 calc.pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaCephaloridineno added zinc (free Zn2+ < 0.4 µM); mono-zinc enzyme0.90.1456.2pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaCephaloridinesaturating zinc concentration110.14575pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaCephaloridine100 µM added zinc110.14576 calc.pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaImipenemno added zinc (free Zn2+ < 0.4 µM); mono-zinc enzyme160.18586pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaImipenemsaturating zinc concentration80.1747pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaImipenem1 µM added zinc14.40.1976 calc.pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaImipenem5 µM added zinc12.50.1966 calc.pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaImipenem10 µM added zinc10.40.1665 calc.pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaImipenem20 µM added zinc90.1753 calc.pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaImipenem50 µM added zinc8.70.1654 calc.pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaImipenem100 µM added zinc8.50.1653 calc.pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaImipenem1000 µM added zinc80.1747 calc.pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaNitrocefinno added zinc (free Zn2+ < 0.4 µM); mono-zinc enzyme0.70.004175pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaNitrocefinsaturating zinc concentration20.0037540pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaNitrocefin2.5 µM added zinc1.360.003450 calc.pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaNitrocefin5 µM added zinc1.520.0035430 calc.pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaNitrocefin10 µM added zinc1.790.003600 calc.pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaNitrocefin50 µM added zinc20.0034590 calc.pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaNitrocefin100 µM added zinc2.060.0037560 calc.pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/N220GAeromonas hydrophilaOxacillin100.4821pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/R121H/N220GAeromonas hydrophilaBenzylpenicillin0.351.50.233pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/R121H/N220GAeromonas hydrophilaCefotaxime0.10.120.833pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/R121H/N220GAeromonas hydrophilaCephaloridine0.20.250.8pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/R121H/N220GAeromonas hydrophilaImipenem1.40.06322pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N116H/R121H/N220GAeromonas hydrophilaNitrocefin0.50.0856pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N220GAeromonas hydrophilaBenzylpenicillin0.060.530.12pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N220GAeromonas hydrophilaCefotaxime0.00910.009pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N220GAeromonas hydrophilaCephaloridine0.035.50.0055pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N220GAeromonas hydrophilaImipenem3900.057.8 × 10³pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant N220GAeromonas hydrophilaNitrocefin0.0080.40.02pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant R121HAeromonas hydrophilaBenzylpenicillin0.11110.01pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant R121HAeromonas hydrophilaCefotaxime0.00170.2450.007pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant R121HAeromonas hydrophilaCephaloridine0.031.80.017pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant R121HAeromonas hydrophilaImipenem3.60.389.5pH 6.5, 30 °COpen
CphA metallo-beta-lactamasevariant R121HAeromonas hydrophilaNitrocefin0.0030.010.3pH 6.5, 30 °COpen