Values CatLog read from this paper
Mechanism of the reaction catalyzed by isoaspartyl dipeptidase from Escherichia coli
PMID 15882050doi:10.1021/bi050008rOpen in Browse
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CatLog read 21 records (59 values) from this paper: isoaspartyl dipeptidase (IAD) from Escherichia coli.
Read from the paper by an AI model and checked against the paper by a second, separate AI run; code converted the units. Values are in CatLog's units; each record opens in Browse with the line the paper prints and the checks.
| Enzyme | Organism | Substrate | kcat (s⁻¹) | Km (mM) | kcat/Km (mM⁻¹ s⁻¹) | Conditions | Open record |
|---|---|---|---|---|---|---|---|
| isoaspartyl dipeptidase (IAD) | Escherichia coli | α-L-aspartyl-L-leucine | 15.7 | 5 | 3.1 | pH 8.1, 30 °C | Open record: isoaspartyl dipeptidase (IAD), α-L-aspartyl-L-leucine |
| isoaspartyl dipeptidase (IAD) | Escherichia coli | β-L-aspartyl-L-alanine | 213 | 3.7 | 58 | pH 8.1, 30 °C | Open record: isoaspartyl dipeptidase (IAD), β-L-aspartyl-L-alanine |
| isoaspartyl dipeptidase (IAD) | Escherichia coli | β-L-aspartyl-L-histidine | 20.8 | 3.7 | 5.6 | pH 8.1, 30 °C | Open record: isoaspartyl dipeptidase (IAD), β-L-aspartyl-L-histidine |
| isoaspartyl dipeptidase (IAD) | Escherichia coli | β-L-aspartyl-L-leucineZn/Zn-bound IAD | 104 | 1.02 | 100 | pH 8.1, 30 °C | Open record: isoaspartyl dipeptidase (IAD), β-L-aspartyl-L-leucine |
| isoaspartyl dipeptidase (IAD) | Escherichia coli | β-L-aspartyl-L-leucineCo/Co-substituted IAD (apo-IAD reconstituted with Co2+) | 34 | 0.62 | 55 | pH 8.1, 30 °C | Open record: isoaspartyl dipeptidase (IAD), β-L-aspartyl-L-leucine |
| isoaspartyl dipeptidase (IAD) | Escherichia coli | β-L-aspartyl-L-leucineCd/Cd-substituted IAD (apo-IAD reconstituted with Cd2+) | 11.9 | 0.36 | 33 | pH 8.1, 30 °C | Open record: isoaspartyl dipeptidase (IAD), β-L-aspartyl-L-leucine |
| isoaspartyl dipeptidase (IAD) | Escherichia coli | β-L-aspartyl-L-leucineNi/Ni-substituted IAD (apo-IAD reconstituted with Ni2+) | 9.2 | 0.09 | 100 | pH 8.1, 30 °C | Open record: isoaspartyl dipeptidase (IAD), β-L-aspartyl-L-leucine |
| isoaspartyl dipeptidase (IAD) | Escherichia coli | β-L-aspartyl-L-lysine | 58 | 0.91 | 63 | pH 8.1, 30 °C | Open record: isoaspartyl dipeptidase (IAD), β-L-aspartyl-L-lysine |
| isoaspartyl dipeptidase (IAD) | Escherichia coli | β-L-aspartyl-L-phenylalanine | 16.9 | 0.23 | 73 | pH 8.1, 30 °C | Open record: isoaspartyl dipeptidase (IAD), β-L-aspartyl-L-phenylalanine |
| isoaspartyl dipeptidase (IAD) | Escherichia coli | β-L-aspartylglycine | 0.93 | 18 | 0.051 | pH 8.1, 30 °C | Open record: isoaspartyl dipeptidase (IAD), β-L-aspartylglycine |
| isoaspartyl dipeptidase (IAD)variant D285A | Escherichia coli | β-L-aspartyl-L-leucine | 6.2 × 10⁻⁴ | 0.5 | 0.0012 | pH 8.1, 30 °C | Open record: isoaspartyl dipeptidase (IAD) variant D285A, β-L-aspartyl-L-leucine |
| isoaspartyl dipeptidase (IAD)variant D285N | Escherichia coli | β-L-aspartyl-L-leucine | 0.017 | 0.98 | 0.018 | pH 8.1, 30 °C | Open record: isoaspartyl dipeptidase (IAD) variant D285N, β-L-aspartyl-L-leucine |
| isoaspartyl dipeptidase (IAD)variant E77D | Escherichia coli | β-L-aspartyl-L-leucine | 0.0051 | 6.9 | 7.4 × 10⁻⁴ | pH 8.1, 30 °C | Open record: isoaspartyl dipeptidase (IAD) variant E77D, β-L-aspartyl-L-leucine |
| isoaspartyl dipeptidase (IAD)variant E77Q | Escherichia coli | β-L-aspartyl-L-leucine | 0.0056 | 0.8 | 0.007 | pH 8.1, 30 °C | Open record: isoaspartyl dipeptidase (IAD) variant E77Q, β-L-aspartyl-L-leucine |
| isoaspartyl dipeptidase (IAD)variant R169K | Escherichia coli | β-L-aspartyl-L-leucine | 9 | 34 | 0.27 | pH 8.1, 30 °C | Open record: isoaspartyl dipeptidase (IAD) variant R169K, β-L-aspartyl-L-leucine |
| isoaspartyl dipeptidase (IAD)variant R169M | Escherichia coli | β-L-aspartyl-L-leucine | — | — | 6.1 × 10⁻⁵ | pH 8.1, 30 °C | Open record: isoaspartyl dipeptidase (IAD) variant R169M, β-L-aspartyl-L-leucine |
| isoaspartyl dipeptidase (IAD)variant R233K | Escherichia coli | β-L-aspartyl-L-leucine | 13 | 20 | 0.53 | pH 8.1, 30 °C | Open record: isoaspartyl dipeptidase (IAD) variant R233K, β-L-aspartyl-L-leucine |
| isoaspartyl dipeptidase (IAD)variant R233M | Escherichia coli | β-L-aspartyl-L-leucine | — | — | 6 | pH 8.1, 30 °C | Open record: isoaspartyl dipeptidase (IAD) variant R233M, β-L-aspartyl-L-leucine |
| isoaspartyl dipeptidase (IAD)variant S289A | Escherichia coli | β-L-aspartyl-L-leucine | 9 | 2.7 | 3.4 | pH 8.1, 30 °C | Open record: isoaspartyl dipeptidase (IAD) variant S289A, β-L-aspartyl-L-leucine |
| isoaspartyl dipeptidase (IAD)variant Y137A | Escherichia coli | β-L-aspartyl-L-leucine | 0.19 | 1.7 | 0.11 | pH 8.1, 30 °C | Open record: isoaspartyl dipeptidase (IAD) variant Y137A, β-L-aspartyl-L-leucine |
| isoaspartyl dipeptidase (IAD)variant Y137F | Escherichia coli | β-L-aspartyl-L-leucine | 0.18 | 1.4 | 0.12 | pH 8.1, 30 °C | Open record: isoaspartyl dipeptidase (IAD) variant Y137F, β-L-aspartyl-L-leucine |